It has been reported in the literature that urinary excretion of formiminoglutamic acid (FIGLU) is increased in rats by vitamin Biz deficiency (1, 2) or by feeding thyroid powder (3). These results with thyroid may be explained on the basis that feeding thyroid powder induces vitamin BIZ deficiency (4, 5 ) which, in turn, produces a secondary folic acid deficiency (2) and thus results in a rise in urinary excretion of FIGLU. Also, it is known that administration of methionine either to vitamin BIZ deficient (1)-or to thyroid-fed rats (3) results in a rapid decrease in the excretion of FIGLU in urine.The addition of methionine to a vitamin B12deficient diet increases considerably the folic acid level of the liver (6), particularly the level of polyglutamate forms (7, 8). In studies with perfused rat liver, Buehring et al. (9) showed that the addition of methionine to the perfusion medium decreased the levels of 5-methyltetrahydrofolate and increased the levels of reduced forms of monoglutamates other than 5-methyl-tetrahydrofolate and also increased the polyglutamates. It was therefore of interest to look further into the relationship between vitamin BIZ, thyroxine, and methionine and study the effect of these compounds on the breakdown of FIGLU in liver homogenates.Materials and Methods. Diets. The composition of basal diet is as follows (g/kg): glucose monohydrate, 714; soy assay protein,z 200; mineral salt mixture (lo), 35; water-soluble vitamins premixed in glucose monohydrate, 10; corn oil with vitamins A, D, and E, 40; and choline chloride, 1. The vitamins were supplied in the following amounts (per kg): vitamin A