Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus

Abstract

We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.

Tissue Culture of Corymbia and Eucalyptus

“…Reduced levels of mineral nutrients have also been used in some establishment media. For example, reduced levels of NH 4 NO 3 and KNO 3 have been used in MS-based establishment media for C. citriodora nodes [80] and greatly reduced CaCl 2 levels have been used during in vitro germination of E. dunnii, E. globulus, and E. saligna seeds [81][82][83][84].…”

“…Glucose at 88 or 176 mM has been used, instead of sucrose, during root induction on E. globulus and E. saligna shoots [139]. Activated charcoal at 83.3-833 mM is often incorporated into the root induction media, including for E. camaldulensis, E. globulus, E. grandis, E. grandis × E. urophylla, E. regnans F.Muell., and E. saligna [14,[82][83][84]88,[139][140][141][142][143][144]. Activated charcoal may act by adsorbing inhibitory compounds, decreasing phenolic oxidation, altering medium pH, or reducing irradiance at the base of the shoot [145,146].…”

“…MS-salt strength has been reduced from half to 3/10 for root induction on E. globulus, E. grandis, and E. saligna shoots [14,[82][83][84]124,139,140]. This includes the use of 3/10-strength MS salts with no Fe, 3 mM Ca, 18 mM NO 3 , and 60 µM Zn for E. globulus shoots [84].…”

“…This includes the use of 3/10-strength MS salts with no Fe, 3 mM Ca, 18 mM NO 3 , and 60 µM Zn for E. globulus shoots [84]. MS macro-salts at quarter-strength with full-strength micro-salts and 1/20-strength Fe.Na.EDTA have also been used for E. globulus, as have half-strength MS salts without NH 4 NO 3 but with quarter-strength Fe.Na.EDTA [157,158].…”

Comparative effects of plant growth promoters and earthworms (Millsonia anomala) on rooting of cocoa orthotropic cuttings

“…As observed in the rooting of cocoa cuttings, the application of auxin in the basal region of stem has been used worldwide to promote the adventitious rooting of woody species (Schwambach et al, 2008;Wendling et al, 2010;Hartmann et al, 2011;Hunt et al, 2011), to influence the micro-cuttings survival and then the rooting of species considered recalcitrant to rooting (Bennett et al, 2003;Schwambach et al, 2005). Beside, many environmental and genetically effects such as nursery practice and source of generative and vegetative materials in different plant species were reported by Dilaver et al (2015) and Yazici and Babalik (2016).…”