Mineral substrates as heterogeneous nucleants in the crystallization of proteins

Paxton, Thomas E.; Sambanis, A.; Rousseau, Ronald W.

doi:10.1016/s0022-0248(98)01231-7

Cited by 18 publications

(12 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researchers have already developed a number of methods to utilize heterogeneous nucleation sites to increase the probability of obtaining crystals and positive results were often reported [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

“…In the field of materials science, heterogeneous nucleation is a widely accepted conventional means to trigger nucleation at lower than supersaturation levels. The positive effects of heterogeneous substrates on crystallization are usually attributed to a lower free energy barrier at the liquid-solid interface [7], a more favorable lattice match [4,5,10], and interactions between the protein molecules and the heterogeneous substrates (including (1) electrostatic interactions [17], (2) nonspecific interactions [19], and (3) hydrophobic interactions [20]). In the current work, we used surfaces etched with HF solutions.…”

Section: Discussionmentioning

confidence: 99%

“…Later, inducing heterogeneous nucleation using various substrates and/or nucleants became a well-known method to increase the probability of crystallization. For example, substrates that act as nucleation sites, such as mineral substrates [5], modified substrates [6], silicon substrates [7,8], and lipid layers [9,10], have been investigated and most effectively improved crystallization. Searching for "universal nucleants" has been researched, and fruitful results have been obtained.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of nucleation during hanging drop protein crystallization using HF Treatment of cover glasses

Guo¹,

Yin²,

Lu³

et al. 2010

Cryst. Res. Technol.

View full text Add to dashboard Cite

We examined a simple approach, i.e., etching cover glasses using hydrofluoric acid (HF), to determine whether cover glass treatment enhances nucleation in hanging drop protein crystallization. Hen egg white lysozyme and proteinase K were used as the model proteins. We found that the treatment increased the success rate of crystallization. The results indicated that the simple treatment, which is easy to adopt without changing much in the hanging drop method, can be utilized as an alternative method to enhance protein crystallization screens.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of nucleation during hanging drop protein crystallization using HF Treatment of cover glasses

Guo¹,

Yin²,

Lu³

et al. 2010

Cryst. Res. Technol.

View full text Add to dashboard Cite

show abstract

“…With respect to the crystallization, a protein‐based biopolymer substrate is typically utilized in nature to develop excellent biomineralization system . Vice versa, the utilization of mineral substrates, chemically modified mica, gel, silanized polystyrene wells, lipid layers, polymeric film surfaces as well as porous media, etc. have contributed a lot to protein crystallization.…”

Section: Introductionmentioning

confidence: 99%

Understanding Biomolecular Crystallization on Amyloid‐Like Superhydrophobic Biointerface

Gao

Tao

et al. 2018

Adv Materials Inter

View full text Add to dashboard Cite

biohybrids. [6] With respect to the crystallization, a protein-based biopolymer substrate is typically utilized in nature to develop excellent biomineralization system. [7] Vice versa, the utilization of mineral substrates, [8] chemically modified mica, [9] gel, [10] silanized polystyrene wells, [11] lipid layers, [12] polymeric film surfaces [13] as well as porous media, [14] etc. have contributed a lot to protein crystallization. Some conjectures have been made from these studies, typically including concentration effect-induced change of supersaturation extent near a surface, [15] adsorption of solutes on a surface, [16] specific interaction between solutes and chemically modified surfaces, [17] the presence of surface microtopography, and spatial characteristics to guide nucleation and crystalline lattice. [18] However, despite immense progress, understanding the crystallization mechanism of proteins and peptides on solid surface remains elusive, and more fundamental clues to reveal this process should be discovered. In this regard, a superhydrophobic surface would be an ideal platform for the crystallization design, since such an interface exhibits great feasibility to concentrate solutes in the sessile droplet [19] and this simplified model induces efficient crystallization of salts, [20] colloidal assembly [21] as well as detection enhancement. [22] Such a platform actually has found a wide range of important applications in current medicine and biotechnology, [23] such as protein adsorption, [24a] drug release, [24b,c] cellular interaction, [24d] and so on. [24e]

show abstract

“…Many efforts have been made in theoretical and experimental investigations to understand the role of physical properties of substrates in promoting crystallization through heterogeneous nucleation [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. It is suggested that the reduction of the free energy of formation of a new solid phase on a solid substrate is due to molecular interactions that take place at the substrate-solution interface [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Influence of electrical potential on the crystallization and adhesion of potassium hydrogen tartrate crystals

2009

View full text Add to dashboard Cite

Interfacial interactions between a hydroalcoholic solution of potassium hydrogen tartrate (KHT) and a stainless steel surface are studied, when an electrical potential is applied to the metal substrate. The capacitive domain of the metal-solution interface is determined by cyclic voltammetry. In order to study the influence of the potential on KHT nucleation and crystal adhesion, the solid-liquid interfacial energy is assessed from contact angle and capillary rise measurements. Experimentally, the contact angle between a NaF solution and a stainless steel vs. the potential has a parabolic behaviour. The metal topography has no apparent influence on physicochemical properties of the interface when ethanol is present in a KHT solution. The metal substrate promotes the formation of KHT crystals, which is improved by the application of an anodic potential. The adhesion of crystals becomes more effective when wires of 25 lm diameter are used in comparison with those of 250 lm.

show abstract

Mineral substrates as heterogeneous nucleants in the crystallization of proteins

Cited by 18 publications

References 2 publications

Enhancement of nucleation during hanging drop protein crystallization using HF Treatment of cover glasses

Enhancement of nucleation during hanging drop protein crystallization using HF Treatment of cover glasses

Understanding Biomolecular Crystallization on Amyloid‐Like Superhydrophobic Biointerface

Influence of electrical potential on the crystallization and adhesion of potassium hydrogen tartrate crystals

Contact Info

Product

Resources

About