Mineralisation of Weathered Crude Oil by a Hydrocarbonoclastic Consortia in Marine Mesocosms

Kadali, Krishna K.; Simons, Keryn L.; Sheppard, Petra J.; Ball, Andrew S.

doi:10.1007/s11270-012-1191-8

Cited by 25 publications

(11 citation statements)

References 44 publications

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“…Various workers have supported the idea of using consortium instead of monoculture to achieve higher biodegradation efficiency (Kadali et al 2012;Shahaby 2014). The co-metabolism of individual members of consortium has been found to have complementary effects on the growth and survival of individual members of the consortium (Sampath et al 2012), which directly or indirectly contributes to stimulated rate of biodegradation.…”

Section: Role Of Inorganic Nutrients and Bulking Agents On Tph Degradmentioning

confidence: 99%

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Kumari

Singh

2016

Int. J. Environ. Sci. Technol.

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Different bacterial and fungal strains, isolated from petroleum hydrocarbon-contaminated soil, were tested, in isolation as well as in combination, for their ability to degrade total petroleum hydrocarbon (TPH) in soil samples spiked with crude oil (2, 5 or 10 %, w/w) for 30 days. The selected combination of bacterial and fungal isolates, i.e., Pseudomonas stutzeri BP10 and Aspergillus niger PS9, exhibited the highest efficiency of TPH degradation (46.7 %) in soil spiked with 2 % crude oil under control condition. Further, when this combination was applied under natural condition in soil spiked with 2 % (w/w) crude oil along with inorganic fertilizers (NPK) and different bulking agents such as rice husk, sugarcane, vermicompost or coconut coir, the percent degradation of TPH was found to be maximum (82.3 %) due to the presence of inorganic fertilizers and rice husk as bulking agent. Further, results showed that the presence of NPK and bulking agents induced the activity of degradative enzymes, such as catalase (0.718 m mol H 2 O 2 g -1 ), laccase (0.77 lmol g -1 ), dehydrogenase (37.5 lg g -1 h -1 ), catechol 1, 2 dioxygenase (276.11 l mol g -1 ) and catechol 2, 3 dioxygenase (15.15 l mol g -1 ) as compared to control (without bioaugmentation). It was inferred that the selected combination microbes along with biostimulants could accentuate the crude oil degradation as evident from the biostimulantinduced enhanced activity of degradative enzymes.

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Section: Role Of Inorganic Nutrients and Bulking Agents On Tph Degradmentioning

confidence: 99%

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Kumari

Singh

2016

Int. J. Environ. Sci. Technol.

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“…PAH-degrading marine bacteria have been used in bioaugmentation approaches to mitigate environmental contamination, but the results have been disappointing so far (Kadali et al, 2012). Complete mineralization tends to be slow (Wang et al, 2008a), or cannot be achieved by a single strain, because of the accumulation of toxic intermediates (Tixier et al, 2002;Festa et al, 2013).…”

Section: Pah-degrading Marine Bacteriamentioning

confidence: 99%

Polycyclic aromatic hydrocarbons in deep sea sediments: Microbe–pollutant interactions in a remote environment

Louvado

Gomes

Simões

et al. 2015

Science of The Total Environment

104

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“…The latter means that a smaller fraction of different species is present in dominant numbers. The 25%, 45% and the 80% curves based on the Y-axis projection of their respective intercepts with the 20% X-axis represent low, medium and high functional organisation respectively [26,28]. Communities of treatment C (seawater + BH medium + weathered crude oil + consortia) in Week 2 and Week 3 suggested that the most active species (playing a major role in degradation) were dominant from the PL curve at 50% and 40% (Figure 3) [28].…”

Section: Pareto-lorenz Curvementioning

confidence: 99%

“…Sea water and weathered crude oil samples were collected from the three treatments C (seawater + BH medium + weathered crude oil + consortia), O (seawater + BH medium + weathered crude oil) and S (seawater + BH medium) obtained from previous work [26] in which consortia comprised of six bacterial strains grown separately and mixed (final OD600 0.04). Samples were collected from the oil-medium interface using sterile 10 mL tubes.…”

Section: Sample Collectionmentioning

confidence: 99%

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RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems

Kadali

Shahsavari

Simons

et al. 2015

JMSE

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Cultivation-independent genomic approaches have greatly advanced our understanding of the ecology and diversity of microbial communities involved in biodegradation processes. However, much still needs to be resolved in terms of the structure, composition and dynamics of the microbial community in impacted ecosystems. Here we report on the RNA activity of the microbial community during the bioremediation process using RNA Temperature Gradient Gel Electrophoresis (RNA-TGGE). Dendrograms constructed from similarity matching data produced from the TGGE profiles separated a community exhibiting high remediation potential. Overall, increased Shannon Weaver Diversity indices (1-2.4) were observed in the high potential remediation treatment samples. The functionality of the microbial community was compared, with the microbial community showing the greatest organisation also showing the highest levels of hydrocarbon degradation. Subsequent sequencing of excised bands from the microbial community identified the presence of Gammaproteobacteria together with a number of uncultured bacteria. The data shows that RNA TGGE represents a simple, reproducible and effective OPEN ACCESSJ. Mar. Sci. Eng. 2015, 3 969 tool for use in the assessment of a commercial bioremediation event, in terms of monitoring either the natural or augmented hydrocarbon-degrading microbial community.

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Mineralisation of Weathered Crude Oil by a Hydrocarbonoclastic Consortia in Marine Mesocosms

Cited by 25 publications

References 44 publications

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Polycyclic aromatic hydrocarbons in deep sea sediments: Microbe–pollutant interactions in a remote environment

RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems

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