Mineralization and osteoblast response to bioactive glassin vitro

Abstract

Bioactive glass, an osteoproductive material, has received considerable attention as a bone graft substitute in the treatment of bony defects. Bioactive CaO-SiO(2)-P(2)O(5) glass was prepared using the sol-gel method, and mineralization behaviour in vitro was investigated by soaking it in simulated body fluid (SBF). Cellular cultivation in vitro, MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) and Von Kossa assays were conducted to evaluate the osteoblast response to the bioactive glass. A calcium phosphate carbonate hydroxide (HCA) layer was formed on the bioactive glass after soaking for 3 days in SBF, which indicated that the mineralization on the surface of bioactive glass could progress spontaneously. The osteoblast response results demonstrated that bioactive glass had no cytotoxicity, and it might not be harmful to the morphology of the osteoblast. The growth and proliferation of the osteoblastic cell could not be inhibited. Nodule formation was also observed in conditioned medium containing dissolution bioactive glass and these nodules were shown to be mineralized by Von Kossa staining, which indicates that bioactive glass shows good biocompatibility.

Simple adaptations of surgical technique to critically reduce the risk of postoperative sternal complications in patients receiving bilateral internal thoracic arteries

“…Sternal complications mainly develop from the distal third of the sternal region, being the most ischaemic area after ITA harvesting and therefore an ideal acid ambience for bacteria ( predominately staphylococcus) to grow [14].…”

Cost-Effectiveness Study of One-Stage Treatment of Chronic Osteomyelitis with Bioactive Glass S53P4

“…This surface makes the glass osteoconductive, with very strong bonds to bone being formed in vivo [7,8,9,10,11]. Additionally, the physicochemical reactions lead to a series of interactions that stimulate new bone formation [12,13,14,15,16,17,18,19,20,21,22]. Because of the aforementioned advantageous properties, bioactive glass is currently used for various clinical indications.…”

“…The formed CaP layer thereafter crystalizes to form hydroxyapatite (HA), the main mineral component of bone. This layer uses bone ingrowth on the surface of the glass [18,20,22].…”

Evaluation of Biological Properties In-Vivo of Poly(L-Lactide-Coglycolide) Composites Containing Bioactive Glass

“…Through interfacial and cell mediated reactions, bioactive glass develops a calcium deficient, carbonated phosphate surface layer that allows it to chemically bond to host bone. This bone-bonding behaviour is referred to as bioactivity and has been associated with the formation of a carbonated hydroxyapatite (HCA) layer on the glass surface when implanted or in contact with biological fluids 15 .…”