Mineralization of a sulfonated textile dye Reactive Red 31 from simulated wastewater using pellets of Aspergillus bombycis

Abstract

BackgroundReactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis strain was isolated from dye contaminated zones of Gujarat Industrial Development Corporation, Vatva, Ahmedabad, India. The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV–visible spectroscopy. Optimization of physicochemical conditions was carried out to achieve maximum decolorization of Reactive Red 31 by fungal pellets.ResultsPellets of A. bombycis strain were found to decolorize this dye (20 mg/L) under aerobic conditions within 12 h. The activity of azoreductase, laccase, phenol oxidase and Manganese peroxidase in fungal culture after decolorization was about 8, 7.5, 19 and 23.7 fold more than before decolorization suggesting that these enzymes might be induced by the addition of Reactive Red 31 dye, and thus results in a higher decolorization. The lab-scale reactor was developed and mineralization of Reactive Red 31 dye by fungal pellets was studied at 6, 12 and 24 h of HRT (hydraulic retention time). At 12 h of HRT, decolorization potential, chemical oxygen demand (COD) and total organic carbon reduction (TOC) was 99.02, 94.19, and 83.97%, respectively, for 20 mg/L of dye concentration.ConclusionsDye decolorization potential of A. bombycis culture was influenced by several factors such as initial dye concentration, biomass concentration, pH, temperature, and required aerated conditions. Induction of azoreductase, laccase, phenol oxidase, and Mn-peroxidase enzymes was observed during dye decolorization phase. A. bombycis pellets showed potential in mineralization of dye in the aerobic reactor system. Isolated fungal strain A. bombycis showed better dye decolorization performance in short duration of time (12 h) as compared to other reported fungal cultures.Graphical abstractDegradation of RR31 dye in developed aerobic fungal pelleted reactor.

High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis

“…Similarly, Chen and Ting [ 48 ] reported that colour removal of Methyl Violet, Crystal Violet and Malachite Green appeared to be better at lower initial dye concentrations by Coriolopsis sp. Khan and Fulekar [ 49 ] simulated wastewater using Aspergillus bombycis for dye decolourisation of a sulfonated textile dye, Reactive Red 31, and demonstrated that after incubation for 12 h, dye concentration was decreased from 99.76 to 91.06% as the concentration increased from 5 to 25 mg L −1 . As they described, the efficiency of the dye decolourisation depended on the initial concentration.…”

A critical review on advances in the practices and perspectives for the treatment of dye industry wastewater

“… [ 132 ] 2 Industrial textile effluent Chaetomium globosum IMA1 KJ472923 [ 133 ] 3 Reactive green dye [RGD] Fungal strain VITAF-1 [ 134 ] 4 Crystal Violet and Cotton Blue Coriolopsis sp. [ 135 ] 5 Congo Red Phoma tropica MRCH and Dichotomomyces cejpii MRCH 1–2 [ 136 ] 6 Crystal violet Trichoderma asperellum [ 137 ] 7 Reactive Red 31 Aspergillus bombycis [ 138 ] 8 Reactive Violet 5 and Reactive Black 5 [diazoic] Trametes trogii [ 139 ] 9 Scarlet RR Peyronellaea prosopidis [ 140 ] 10 Different azo dyes Aspergillus flavus [ 120 ] 11 Poly R Pseudoglarobasidium acaciicola [ 141 ] 12 Direct Red 128 and Direct Blue ...…”

Microbe-mediated remediation of dyes: Current status and future challenges

“…Barapatre et al [82] reported Aspergillus flavus for biodegradation of Malachite Green in which intermediate N-demethylated and N-oxidized metabolites were identified [Figure 1]. Fungal strain Aspergillus bombycis was reported for having the ability to degrade Reactive Red 31 [83]. Asses et al [84] reported Aspergillus niger having the ability to decolorize Congo red (200 mg/L) within 6 days of incubation [Figure 2].…”