Mineralization-related modifications in the calcifying tendons of turkey (Meleagris gallopavo)

Raspanti, Mario; Reguzzoni, Marcella; Protasoni, Marina; Congiu, Terenzio

doi:10.1016/j.micron.2015.01.003

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…Although extensive mineralisation of tendon in mammals is pathological, the mineral plays a vital role in the formation of the tendon-bone interfaces. Many aspects of the mineralisation process are similar, including: assembly of the type I collagen matrix, hydroxylation of the possible nucleation sites, formation of crosslinks, nucleation and crystallisation of the mineral into platelets 5 , 6 . A more complete appreciation of the mineralisation process in tendon will inform our understanding of how mineral forms in collagenous tissues.…”

Section: Introductionmentioning

confidence: 99%

Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

Kłosowski

Carzaniga

Shefelbine

et al. 2018

Sci Rep

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The macro- and micro-structures of mineralised tissues hierarchy are well described and understood. However, investigation of their nanostructure is limited due to the intrinsic complexity of biological systems. Preceding transmission electron microscopy studies investigating mineralising tissues have not resolved fully the initial stages of mineral nucleation and growth within the collagen fibrils. In this study, analytical scanning transmission electron microscopy and electron energy-loss spectroscopy were employed to characterise the morphology, crystallinity and chemistry of the mineral at different stages of mineralization using a turkey tendon model. In the poorly mineralised regions, calcium ions associated with the collagen fibrils and ellipsoidal granules and larger clusters composed of amorphous calcium phosphate were detected. In the fully mineralised regions, the mineral had transformed into crystalline apatite with a plate-like morphology. A change in the nitrogen K-edge was observed and related to modifications of the functional groups associated with the mineralisation process. This transformation seen in the nitrogen K-edge might be an important step in maturation and mineralisation of collagen and lend fundamental insight into how tendon mineralises.

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Section: Introductionmentioning

confidence: 99%

Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

Kłosowski

Carzaniga

Shefelbine

et al. 2018

Sci Rep

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“…The existing microscopic research of tendons is focused on humans (Benjamin, Kaiser, & Milz, ), rats (Raspanti, Manelli, Franchi, & Ruggeri, ), horses (Thorpe, Spiesz, Chaudhry, Screen, & Clegg, ), and turkeys (Raspanti, Reguzzoni, Protasoni, & Congiu, ). Ligaments and tendons were often considered or studied as tissues with a similar hierarchical collagen structure (Hankemeier et al, ; Hoffmann & Gross, ; Provenzano & Vanderby, ).…”

Section: Introductionmentioning

confidence: 99%

Macroscopic and microscopic analyses in flexor tendons of the tarsometatarso‐phalangeal joint of ostrich (Struthio camelus) foot with energy storage and shock absorption

Zhang

Han

Luo

et al. 2017

Journal of Morphology

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Flexor tendons function as energy storage and shock absorption structures in the tarsometatarso-phalangeal joint (TMTPJ) of ostrich feet during high-speed and heavy-load locomotion. In this study, mechanisms underlying the energy storage and shock absorption of three flexor tendons of the third toe were studied using histology and scanning electron microscopy (SEM). Macroscopic and microscopic structures of the flexor tendons in different positions of TMTPJ were analyzed. Histological slices showed collagen fiber bundles of all flexor tendons in the middle TMTPJ were arranged in a linear-type, but in the proximal and distal TMTPJ, a wavy-type arrangement was found in the tendon of the M. flexor digitorum longus and tendon of the M. flexor perforans et perforatus digiti III, while no regular-type was found in the tendon of the M. flexor perforatus digiti III. SEM showed that the collagen fiber bundles of flexor tendons were arranged in a hierarchically staggered way (horizontally linear-type and vertically linear-type). Linear-type and wavy-type both existed in the proximal TMTPJ for the collagen fiber bundles of the tendon of the M. flexor perforatus digiti III, but only the linear-type was found in the distal TMTPJ. A number of fibrils were distributed among the collagen fiber bundles, which were likely effective in connection, force transmission and other functions. The morphology and arrangement of collagen fiber bundles were closely related to the tendon functions. We present interpretations of the biological functions in different positions and types of the tendons in the TMTPJ of the ostrich feet.

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“…These results confirm some heterogeneity among the studies and consequent discussion about the factors which influence the crimp pattern parameters and development, for instance, cell–matrix interactions, exercise or increased number of cross-links. Not only is development of the crimp pattern still a subject of discussion, but its function is also under question (Raspanti et al, 2015). The most frequent suggestion for the function of the crimp pattern is that it acts as a mechanical buffer (Franchi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Calcaneal Tendon Collagen Fiber Morphometry and Aging

et al. 2017

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Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (~7 days) to elderly (~38 months) rabbits were monitored in intact (n tendons=24) and microtome sectioned (n tendons=11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Λ) started at very low values (A=2.0±0.6 µm, Λ=19±4 µm) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development.

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Mineralization-related modifications in the calcifying tendons of turkey (Meleagris gallopavo)

Cited by 5 publications

References 41 publications

Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

Macroscopic and microscopic analyses in flexor tendons of the tarsometatarso‐phalangeal joint of ostrich (Struthio camelus) foot with energy storage and shock absorption

Calcaneal Tendon Collagen Fiber Morphometry and Aging

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