Minerals in the pre-settled coral Stylophora pistillata crystallize via protein and ion changes

Akiva, Anat; Neder, Maayan; Kahil, Keren; Gavriel, Rotem; Pinkas, Iddo; Goobes, Gil; Mass, Tali

doi:10.1038/s41467-018-04285-7

Cited by 62 publications

(91 citation statements)

References 47 publications

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“…Among the greatest advantages of Raman spectroscopy is its applicability on small spatial and temporal scales, enabling characterization of near-instantaneous skeletal growth and micro-banding patterns produced on various timescales. Although our initial applications provided no evidence for a role of ACC particles in calcification by a range of taxa, Raman spectroscopy can now be readily applied to search for ACC in various developmental stages and under different environmental conditions (Akiva et al, 2018;Foster & Clode, 2016).…”

Section: Discussionmentioning

confidence: 99%

Investigating marine bio‐calcification mechanisms in a changing ocean with in vivo and high‐resolution ex vivo Raman spectroscopy

DeCarlo

Comeau

Cornwall

et al. 2019

Global Change Biology

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Ocean acidification poses a serious threat to marine calcifying organisms, yet experimental and field studies have found highly diverse responses among species and environments. Our understanding of the underlying drivers of differential responses to ocean acidification is currently limited by difficulties in directly observing and quantifying the mechanisms of bio‐calcification. Here, we present Raman spectroscopy techniques for characterizing the skeletal mineralogy and calcifying fluid chemistry of marine calcifying organisms such as corals, coralline algae, foraminifera, and fish (carbonate otoliths). First, our in vivo Raman technique is the ideal tool for investigating non‐classical mineralization pathways. This includes calcification by amorphous particle attachment, which has recently been controversially suggested as a mechanism by which corals resist the negative effects of ocean acidification. Second, high‐resolution ex vivo Raman mapping reveals complex banding structures in the mineralogy of marine calcifiers, and provides a tool to quantify calcification responses to environmental variability on various timescales from days to years. We describe the new insights into marine bio‐calcification that our techniques have already uncovered, and we consider the wide range of questions regarding calcifier responses to global change that can now be proposed and addressed with these new Raman spectroscopy tools.

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Section: Discussionmentioning

confidence: 99%

Investigating marine bio‐calcification mechanisms in a changing ocean with in vivo and high‐resolution ex vivo Raman spectroscopy

DeCarlo

Comeau

Cornwall

et al. 2019

Global Change Biology

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show abstract

“…In order to obtain a suitable geometric structure and inlet flow rate, the flow characteristic was analyzed based on Equations (2) and (3). In this case, geometric parameters for the microwell, buffer region, and barrier were used to optimize the microfluidic chip design for culture of the individual coral polyp, as shown in Figure 2a.…”

Section: Optimization Design Of Microfluidic Chip Structurementioning

confidence: 99%

“…Coral reefs are an important component of the marine ecosystem, where they have a great impact on maintaining global dynamic ecological balance [1][2][3][4][5]. The existential crisis of coral reefs has arisen, as the marine environment is changing, including ocean acidification and seawater temperature increase, resulted from the high carbon dioxide levels and El Nino phenomenon [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…The result indicated that the feeding can improve the coral health and support the metabolic process during and after thermal stress [18]. However, current studies related to the effect of thermal stress on the coral bleaching mainly focus on the static culture of coral tissues or small branchs [3,13,19]. The accumulation of metabolic waste, consumption of dissolved oxygen (DO), and dissolved inorganic carbon (DIC) can cause the separation of the zooxanthellae from the coral host cells, and thus affect the reliability of experimental results [20].…”

Section: Introductionmentioning

confidence: 99%

“…This is a limiting factor for evaluating the effect of the environmental stress and may lead to failure of the experimental test. In addition, the individual differences of corals are another key factor to evaluate the effect of the environmental stress [3]. Therefore, there is a desperate need to develop a flexible integrated microdevice as an alternative to traditional methods to mimic the marine ecological environment.…”

Section: Introductionmentioning

confidence: 99%

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Miniaturized Platform for Individual Coral Polyps Culture and Monitoring

Luo

Zhao

et al. 2020

Micromachines

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Methodologies for coral polyps culture and real-time monitoring are important in investigating the effects of the global environmental changes on coral reefs and marine biology. However, the traditional cultivation method is limited in its ability to provide a rapid and dynamic microenvironment to effectively exchange the chemical substances and simulate the natural environment change. Here, an integrated microdevice with continuous perfusion and temperature-control in the microenvironment was fabricated for dynamic individual coral polyps culture. For a realistic mimicry of the marine ecological environment, we constructed the micro-well based microfluidics platform that created a fluid flow environment with a low shear rate and high substance transfer, and developed a sensitive temperature control system for the long-term culture of individual coral polyps. This miniaturized platform was applied to study the individual coral polyps in response to the temperature change for evaluating the coral death caused by El Nino. The experimental results demonstrated that the microfluidics platform could provide the necessary growth environment for coral polyps as expected so that in turn the biological activity of individual coral polyps can quickly be recovered. The separation between the algae and host polyp cells were observed in the high culture temperature range and the coral polyp metabolism was negatively affected. We believe that our culture platform for individual coral polyps can provide a reliable analytical approach for model and mechanism investigations of coral bleaching and reef conservation.

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Crystallization in Confinement

2020

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Many crystallization processes of great importance, including frost heave, biomineralization, the synthesis of nanomaterials, and scale formation, occur in small volumes rather than bulk solution. Here, the influence of confinement on crystallization processes is described, drawing together information from fields as diverse as bioinspired mineralization, templating, pharmaceuticals, colloidal crystallization, and geochemistry. Experiments are principally conducted within confining systems that offer well‐defined environments, varying from droplets in microfluidic devices, to cylindrical pores in filtration membranes, to nanoporous glasses and carbon nanotubes. Dramatic effects are observed, including a stabilization of metastable polymorphs, a depression of freezing points, and the formation of crystals with preferred orientations, modified morphologies, and even structures not seen in bulk. Confinement is also shown to influence crystallization processes over length scales ranging from the atomic to hundreds of micrometers, and to originate from a wide range of mechanisms. The development of an enhanced understanding of the influence of confinement on crystal nucleation and growth will not only provide superior insight into crystallization processes in many real‐world environments, but will also enable this phenomenon to be used to control crystallization in applications including nanomaterial synthesis, heavy metal remediation, and the prevention of weathering.

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Minerals in the pre-settled coral Stylophora pistillata crystallize via protein and ion changes

Cited by 62 publications

References 47 publications

Investigating marine bio‐calcification mechanisms in a changing ocean with in vivo and high‐resolution ex vivo Raman spectroscopy

Investigating marine bio‐calcification mechanisms in a changing ocean with in vivo and high‐resolution ex vivo Raman spectroscopy

Miniaturized Platform for Individual Coral Polyps Culture and Monitoring

Crystallization in Confinement

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