Mini-review: advances in 3D bioprinting of vascularized constructs

Bova, Lorenzo; Billi, Fabrizio; Cimetta, Elisa

doi:10.1186/s13062-020-00273-4

Cited by 21 publications

(18 citation statements)

References 37 publications

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“…Different from the “top-down” approaches, “bottom-up” approaches start from constructing fabricating the micro modular tissues with cells and biocompatible materials[ 22 - 32 ]. As shown in Figure 1 , 1D or 2D modules such as spheroids, rings especially for engineering microvessels, fibers, plates with arbitrary shapes, and cell sheets can be fabricated through cell aggregation and microfabrication techniques with mass production.…”

Section: Engineering Microvessels From the Bottom Upmentioning

confidence: 99%

“…Modular tissue engineering adopting the “bottom-up” approach builds one-dimensional (1D) or two-dimensional (2D) modular tissues in micro scale first and then uses these modules as building blocks to generate large tissues and organs[ 22 - 32 ]. It allows recreating complex but indispensable microstructural features of the engineered tissues.…”

Section: Introductionmentioning

confidence: 99%

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Bio-assembling and Bioprinting for Engineering Microvessels from the Bottom Up

Liu

Yue

Kojima

et al. 2024

IJB

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Blood vessels are essential in transporting nutrients, oxygen, metabolic wastes, and maintaining the homeostasis of the whole human body. Mass of engineered microvessels is required to deliver nutrients to the cells included in the constructed large three-dimensional (3D) functional tissues by diffusion. It is a formidable challenge to regenerate microvessels and build a microvascular network, mimicking the cellular viabilities and activities in the engineered organs with traditional or existing manufacturing techniques. Modular tissue engineering adopting the “bottom-up” approach builds one-dimensional (1D) or two-dimensional (2D) modular tissues in micro scale first and then uses these modules as building blocks to generate large tissues and organs with complex but indispensable microstructural features. Building the microvascular network utilizing this approach could be appropriate and adequate. In this review, we introduced existing methods using the “bottom-up” concept developed to fabricate microvessels including bio-assembling powered by different micromanipulation techniques andbioprinting utilizing varied solidification mechanisms. We compared and discussed the features of the artificial microvessels engineered by these two strategies from multiple aspects. Regarding the future development of engineering the microvessels from the bottom up, potential directions were also concluded.

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Section: Engineering Microvessels From the Bottom Upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bio-assembling and Bioprinting for Engineering Microvessels from the Bottom Up

Liu

Yue

Kojima

et al. 2024

IJB

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“…However, there are also risks and ethical issues that should be considered, especially when 3D bioprinting technology is aimed at tissue engineering purposes. In this case, the composition of biological inks raises some concerns, not only associated with the security of the grafts implanted, but also related to the biological origin of such cells [3]. In the clearest scenario, where patient's autologous cells are included in the bio-ink composition, a random migration of cells from implanted ocular grafts could arise in different parts of the body, leading to potential undesired effects [5,8].…”

Section: Ethical Issues and Commercialization Regulatory Aspectsmentioning

confidence: 99%

“…The development of new revolutionary technologies during recent years, such as the use of Big Data, virtual reality systems and three-dimensional (3D) bioprinting, has created great expectations in the scientific community, not only regarding the improvement of the quality of life in patients affected by devastating pathologies, but also in terms of saving health-care associated resources [1][2][3]. In this regard, 3D bioprinting is an emerging manufacturing technology which holds great promise for a wide variety of biomedical applications, including drug testing, pathophysiological studies and regenerative medicine [4].…”

Section: Introductionmentioning

confidence: 99%

Current Insights into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration

et al. 2021

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Three-dimensional (3D) printing is a game changer technology that holds great promise for a wide variety of biomedical applications, including ophthalmology. Through this emerging technique, specific eye tissues can be custom-fabricated in a flexible and automated way, incorporating different cell types and biomaterials in precise anatomical 3D geometries. However, and despite the great progress and possibilities generated in recent years, there are still challenges to overcome that jeopardize its clinical application in regular practice. The main goal of this review is to provide an in-depth understanding of the current status and implementation of 3D bioprinting technology in the ophthalmology field in order to manufacture relevant tissues such as cornea, retina and conjunctiva. Special attention is paid to the description of the most commonly employed bioprinting methods, and the most relevant eye tissue engineering studies performed by 3D bioprinting technology at preclinical level. In addition, other relevant issues related to use of 3D bioprinting for ocular drug delivery, as well as both ethical and regulatory aspects, are analyzed. Through this review, we aim to raise awareness among the research community and report recent advances and future directions in order to apply this advanced therapy in the eye tissue regeneration field.

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“…Commonly used sacrificial gels include Pluronic F-127, carbohydrate-glass, gelatin, and agarose. 101 Early developments and printing of microchannels occurred used FRESH printing and similar methods. 102,59 Hinton and colleagues used FRESH to print an MRI-derived coronary artery system with <15% variability in dimensions.…”

Section: Vasculaturementioning

confidence: 99%

Moving Towards Functional Bioprinting

Thomas¹,

Mettenbrink²

2021

Preprint

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3D bioprinting technologies are rapidly developing and provide a platform for manufacturing structures that mimic the in vivo environment. Recent research aims to produce 3D bioprinted structures that recapitulate both in vivo structure and functionality. Advancements in both producing high fidelity and functional structures pave the way for full organ bioprinting. Full organ bioprinting holds promise for patients facing renal diseases given both the limited availability of donor kidneys for transplantation which offers the highest quality of life for patients facing renal failure. While the generation of a fully functional bioprinted kidney is a long-term goal, the first step is generating bioprinted functional renal tissue.Functional bioprinted renal tissue may pave the way for full scale organ printing and may offer a more accurate in vitro model for testing the renal toxicity of newly developed therapeutics which holds promise given the limitations of current preclinical in vivo and in vitro models to accurately predict renal toxicity of newly developed therapeutics in humans. Recent work showcases advancements toward renal bioprinting and advancements in the field of bioprinting more broadly may provide opportunities for advancement in renal bioprinting. This review aims to cover recent advances in renal bioprinting and opportunities for innovation. The review seeks to address the mechanical, biological and translational aspects of bioprinting functional renal tissue through an overview of recent advancements (last 5 years) in developing bioinks, utilizing existing 3D bioprinting methods to produce high fidelity printed structures, supporting viability, cell adhesion, cell distribution, functionality and vascularization, and considering important translational aspects of renal bioprinting including larger-scale printing, clinical potential, and prospects towards whole organ generation.

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Mini-review: advances in 3D bioprinting of vascularized constructs

Cited by 21 publications

References 37 publications

Bio-assembling and Bioprinting for Engineering Microvessels from the Bottom Up

Bio-assembling and Bioprinting for Engineering Microvessels from the Bottom Up

Current Insights into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration

Moving Towards Functional Bioprinting

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