Miniature microscopes for large-scale imaging of neuronal activity in freely behaving rodents

Ziv, Yaniv; Ghosh, Kunal

doi:10.1016/j.conb.2015.04.001

Cited by 79 publications

(68 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the anatomical and genetic targeting of neural circuitry elements or connectivity can be combined with activity readouts and/or activity manipulations in behaviorally relevant contexts in order to form a complete picture of causal circuitry functions in relation to behavior. In vivo neural activity imaging in freely behaving mice using miniature microscopes for activity readouts [67] and optogenetic/chemogenetic approaches for activity manipulations [68, 69] are representative tools that are currently available (Figure 3). …”

Section: Towards Better Understanding Of the Physiological And Patholmentioning

confidence: 99%

The Insula: An Underestimated Brain Area in Clinical Neuroscience, Psychiatry, and Neurology

Namkung

Kim

Sawa

2017

Trends in Neurosciences

338

226

View full text Add to dashboard Cite

Supported by recent human neuroimaging studies, the insula is re-emerging as an important brain area not only in the physiological understanding of the brain, but also in pathological contexts in clinical research. Here, we briefly introduce the anatomical and histological features of the human insula. We then summarize the physiological functions of the insula and underscore its pathological roles in psychiatric and neurological disorders that have long been underestimated. We finally propose possible strategies through which the role of the insula may be further understood for both basic and clinical neuroscience.

show abstract

Section: Towards Better Understanding Of the Physiological And Patholmentioning

confidence: 99%

The Insula: An Underestimated Brain Area in Clinical Neuroscience, Psychiatry, and Neurology

Namkung

Kim

Sawa

2017

Trends in Neurosciences

338

226

View full text Add to dashboard Cite

show abstract

“…In addition, our imaging approach clearly distinguishes changes in the 287 frequency and amplitude of Ca 2+ transients per active neuron from changes in the overall number 288 of active cells(Fig. 3a), which can more easily be conflated with one-photon endoscopic 289techniques(Helmchen & Denk 2005;Ziv & Ghosh 2015;Zhou et al, 2018). Accordingly, we ascribe 290 most of the changes in Ca 2+ event rate observed at the population level(Fig.…”

mentioning

confidence: 78%

Dopamine differentially modulates the size of projection neuron ensembles in the intact and dopamine-depleted striatum

Maltese

March

Bashaw

et al. 2019

Preprint

View full text Add to dashboard Cite

12 13 14 15 SUMMARY 16 17Dopamine (DA) is a critical modulator of brain circuits that control voluntary movements, but our 18 understanding of its influence on the activity of target neurons in vivo remains limited. Here, we 19 use two-photon Ca 2+ imaging to simultaneously monitor the activity of direct and indirect-20 pathway spiny projection neurons (SPNs) in the striatum of behaving mice during acute and 21 prolonged manipulations of DA signaling. We find that, contrary to prevailing models, DA does 22 not modulate activity rates in either pathway strongly or differentially. Instead, DA exerts a 23 prominent influence on the overall number of direct and indirect pathway SPNs recruited during 24behavior. Chronic loss of midbrain DA neurons in a model of Parkinson's disease selectively 25 impacts direct pathway ensembles and profoundly alters how they respond to DA elevation. Our 26 results indicate that DA regulates striatal output by dynamically reconfiguring its sparse 27 ensemble code and provide novel insights into the pathophysiology of Parkinson's disease.

show abstract

“…As a result, fluctuations in fluorescent emission over time can be used to infer dynamic changes in cellular activity. Moreover, when these signals are visualized with cellular-level resolution, fluorescent indicators of neural activity provide not only temporal information related to dynamic changes in brain cell activity 17 , but they also enable the visualization of each cells spatial location within the brain 18 .…”

Section: Introductionmentioning

confidence: 99%

Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses

et al. 2016

View full text Add to dashboard Cite

Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly expanded our understanding of the brain. However, due to light scattering properties of the brain as well as the size and rigidity of traditional imaging technology, in vivo calcium imaging has been limited to superficial brain structures during head fixed behavioral tasks. This limitation can now be circumvented by utilizing miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thus permitting optical access to deep brain (or superficial) neural ensembles during naturalistic behaviors. Here, we describe procedural steps to conduct such imaging studies using mice. However, we anticipate the protocol can be easily adapted for use in other small vertebrates. Successful completion of this protocol will permit cellular imaging of neuronal activity and the generation of data sets with sufficient statistical power to correlate neural activity with stimulus presentation, physiological state, and other aspects of complex behavioral tasks. This protocol takes 6–11 weeks to complete.

show abstract

Miniature microscopes for large-scale imaging of neuronal activity in freely behaving rodents

Cited by 79 publications

References 58 publications

The Insula: An Underestimated Brain Area in Clinical Neuroscience, Psychiatry, and Neurology

The Insula: An Underestimated Brain Area in Clinical Neuroscience, Psychiatry, and Neurology

Dopamine differentially modulates the size of projection neuron ensembles in the intact and dopamine-depleted striatum

Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses

Contact Info

Product

Resources

About