Stem water content is a pivotal parameter that characterizes the vitality of plants and maintains their internal water balance. Given the insu cient comprehension regarding the stem water content characteristics and its in uencing factors during different stages of the overwintering period, the study, focusing on Acer truncatum., developed an Internet of Things (IoT)-based ecological information monitoring system. The system incorporated a proprietary stem water content sensor, allowing non-invasive, in-situ and real time acquisition of stem water content while monitoring diverse environmental parameters. We conducted a detailed elucidation of stem water content variation characteristics and its responses to diverse environmental factors. The results shouwed: (1) During the overwintering period, stem water content exhibited diurnal variations characterized by " daytime ascent and nighttime descent" across the three stages, exhibiting differences in the moment when the stem water content reaching extremal values and daily uctuations ranges. Stem water content exhibited minimal uctuations during deciduous and budbreaking stages but experienced signi cant freezing-thawing alternations during the dormant stage, leading to increased daily uctuation range. (2) Pearson correlation coe cients between environmental parameters and stem water content varied dynamically across stages. Path analysis revealed: during the deciduous stage, stem temperature and saturation vapor pressure de cit were dominant factors in uencing stem water content; during dormant stage, air temperature and saturation vapor pressure de cit directly impacted stem water content; during the bud-breaking stage, the primary parameters affecting stem water content were saturation vapor pressure de cit and stem temperature. The study provides valuable insights into unveiling the water transport patterns within tree stems tissue and their environmental adaptation mechanisms during the overwintering period, aiding in the scienti c development of winter management strategies to protect trees from severe cold and freezing damage, while fostering healthy growth in the subsequent year.