Miniature Untethered EEG Recorder Improves Advanced Neuroscience Methodologies

Crispin-Bailey, Christopher; Austin, Jim; Platt, Bettina; Moulds, Anthony; Crouch, Barry

doi:10.1109/tbcas.2019.2935298

Cited by 5 publications

(5 citation statements)

References 43 publications

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“…Avoiding the use of tethered EEG equipment was a pre-requisite for the successful set-up of sleep recordings in the PhenoTyper. A number of biosensors for data logging have been developed, for example the Neurologger (Vyssotski et al, 2006;Jyoti et al, 2010Jyoti et al, , 2015Platt et al, 2011;Goonawardena et al, 2015) and the NAT-1 (Crouch et al, 2018(Crouch et al, , 2019Crispin-Bailey et al, 2019). Both are wearable ultra-miniature devices of light weight (<3 g), record four channels at ≥200 Hz sampling frequency, have on board memory of more than 512 MB, come with infrared (IR) sensors for event-synchronization, carry a 2D or 3D accelerometer making implantation of electromyographic electrodes obsolete, and record for up to 7 days.…”

Section: Expanding the Home Cage: An Example With Eeg Recordingmentioning

confidence: 99%

Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives

Grieco

Bernstein

Biemans

et al. 2021

Front. Behav. Neurosci.

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The reproducibility crisis (or replication crisis) in biomedical research is a particularly existential and under-addressed issue in the field of behavioral neuroscience, where, in spite of efforts to standardize testing and assay protocols, several known and unknown sources of confounding environmental factors add to variance. Human interference is a major contributor to variability both within and across laboratories, as well as novelty-induced anxiety. Attempts to reduce human interference and to measure more "natural" behaviors in subjects has led to the development of automated home-cage monitoring systems. These systems enable prolonged and longitudinal recordings, and provide large continuous measures of spontaneous behavior that can be analyzed across multiple time scales. In this review, a diverse team of neuroscientists and product developers share their experiences using such an automated monitoring system that combines Noldus PhenoTyper® home-cages and the video-based tracking software, EthoVision® XT, to extract digital biomarkers of motor, emotional, social and cognitive behavior. After presenting our working definition of a “home-cage”, we compare home-cage testing with more conventional out-of-cage tests (e.g., the open field) and outline the various advantages of the former, including opportunities for within-subject analyses and assessments of circadian and ultradian activity. Next, we address technical issues pertaining to the acquisition of behavioral data, such as the fine-tuning of the tracking software and the potential for integration with biotelemetry and optogenetics. Finally, we provide guidance on which behavioral measures to emphasize, how to filter, segment, and analyze behavior, and how to use analysis scripts. We summarize how the PhenoTyper has applications to study neuropharmacology as well as animal models of neurodegenerative and neuropsychiatric illness. Looking forward, we examine current challenges and the impact of new developments. Examples include the automated recognition of specific behaviors, unambiguous tracking of individuals in a social context, the development of more animal-centered measures of behavior and ways of dealing with large datasets. Together, we advocate that by embracing standardized home-cage monitoring platforms like the PhenoTyper, we are poised to directly assess issues pertaining to reproducibility, and more importantly, measure features of rodent behavior under more ethologically relevant scenarios.

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Section: Expanding the Home Cage: An Example With Eeg Recordingmentioning

confidence: 99%

Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives

Grieco

Bernstein

Biemans

et al. 2021

Front. Behav. Neurosci.

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“…Recent advances in miniaturization of wireless EEG recording devices (Crispin-Bailey et al, 2013;Etholm et al, 2010) enable brain activity to be recorded in freely moving animals in a range of environments. However, the combination of behavioural / cognitive testing and qEEG in rodents is arguably more complex than in human subjects.…”

Section: Challenges In Pre-clinical Behavioural Eegmentioning

confidence: 99%

“…EEG activity was recorded at a sampling rate of 200 Hz using the NAT-1 wireless recording device (Cybula, UK; (Crispin-Bailey et al, 2013)) during a Y-maze spontaneous alternation task. NAT-1 devices were attached under gentle manual restraint and animals were allowed 30 minutes habituation to the device and experimental room prior to testing.…”

Section: Behavioural Testing + Eeg Re Cordingmentioning

confidence: 99%

Of mice and motion: Behavioural-EEG phenotyping of Alzheimer’s disease mouse models

Crouch

Yeap

Pais

et al. 2019

Journal of Neuroscience Methods

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“…With advancements made in preclinical research, laboratories often align the mechanistic and functional aspects of animal behavioural outcomes to that of dynamic neuronal events. This is often performed with tethered or, more recently, wireless miniature recording systems [1][2][3][4][5][6][7][8] to allow recording of electroencephalography (EEG) in freely moving animals with minimal discomfort. In our laboratory, this includes the bilateral implantation of gold electrodes into the skull and attachment of a lightweight Neural Activity Tracker (NAT-1; Cybula, UK) device to this head-stage prior to behavioural testing.…”

Section: Introductionmentioning

confidence: 99%

Mouse exploratory behaviour in the open field with and without NAT-1 EEG device: Effects of MK801 and scopolamine

Lim,

Bray,

Janhunen

et al. 2024

Preprint

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One aspect of reproducibility in preclinical research that is frequently overlooked is the physical condition in which physiological, pharmacological or behavioural recordings are conducted. In this study, the physical conditions of mice were altered through the attachments of wireless electrophysiological recording devices (Neural Activity Tracker-1, NAT-1). NAT-1 devices are miniaturised multichannel devices with on-board memory for direct high-resolution recording of brain activity for >48 hrs. Such devices may limit the mobility of animals and affect their behavioural performance due to the added weight (total weight of approximately 3.4 g). Mice were additionally treated with saline (control), N-methyl-D-aspartate (NMDA) receptor antagonist MK801 (0.85 mg/kg), or the muscarinic acetylcholine receptor blocker scopolamine (0.65 mg/kg) to allow exploration of the effect of NAT-1 attachments in pharmacologically treated mice. We found only minimal differences in behavioural outcomes with NAT-1 attachments in standard parameters of locomotor activity widely reported for the open field test between drug-treatments. Hypoactivity was globally observed as a consistent outcome in MK801-treated subjects and hyperactivity in scopolamine groups regardless of NAT-1 attachments. These data collectively confirm the reproducibility for combined behavioural, pharmacological and physiological endpoints even in the presence of lightweight wireless data loggers. The NAT-1 therefore constitutes a pertinent tool for investigating brain activity in e.g. drug discovery, models of neuropsychiatric and/or neurodegenerative diseases with minimal effects on pharmacological and behavioural outcomes.

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Miniature Untethered EEG Recorder Improves Advanced Neuroscience Methodologies

Cited by 5 publications

References 43 publications

Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives

Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives

Of mice and motion: Behavioural-EEG phenotyping of Alzheimer’s disease mouse models

Mouse exploratory behaviour in the open field with and without NAT-1 EEG device: Effects of MK801 and scopolamine

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