Miniaturization of electroanalytical systems

Matysik, Frank‐Michael

doi:10.1007/s00216-002-1635-x

Cited by 40 publications

(18 citation statements)

References 21 publications

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“…EC detection, since the first reported in 1998 by Wolley et al [15], has been employed successfully in CEmicrochips. It has important advantages such as inherent miniaturization, sensitivity, low cost, portability, and compatibility with microfabrication technology [16]. Different modes of EC detection have been reported including amperometry, conductometry, and potentiometry.…”

Section: Introductionmentioning

confidence: 99%

Poly(methylmethacrylate) and Topas capillary electrophoresis microchip performance with electrochemical detection

2005

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A capillary electrophoresis (CE) microchip made of a new and promising polymeric material: Topas (thermoplastic olefin polymer of amorphous structure), a cyclic olefin copolymer with high chemical resistance, has been tested for the first time with analytical purposes, employing an electrochemical detection. A simple end-channel platinum amperometric detector has been designed, checked, and optimized in a poly-(methylmethacrylate) (PMMA) CE microchip. The end-channel design is based on a platinum wire manually aligned at the exit of the separation channel. This is a simple and durable detection in which the working electrode is not pretreated. H(2)O(2) was employed as model analyte to study the performance of the PMMA microchip and the detector. Factors influencing migration and detection processes were examined and optimized. Separation of H(2)O(2) and L-ascorbic acid (AsA) was developed in order to evaluate the efficiency of microchips using different buffer systems. This detection has been checked for the first time with a microchip made of Topas, obtaining a good linear relationship for mixtures of H(2)O(2) and AsA in different buffers.

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Section: Introductionmentioning

confidence: 99%

Poly(methylmethacrylate) and Topas capillary electrophoresis microchip performance with electrochemical detection

2005

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“…Although it is important in all fields, it is of special relevance in the area of biosensors. The simplicity of the electrochemical instrumentation and the type of measurement that is made adapt well to miniaturization requirements (Matisyk 2003).…”

Section: Instrumentsmentioning

confidence: 99%

Label-Free DNA Biosensor for Electrochemical Detection of Short DNA Sequences Related to Human Papilloma Virus

2009

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Highly sensitive label-free techniques of DNA determination are particularly interesting in relation to the present development of an electrochemical hybridization biosensor for the detection of short DNA fragments specific to the human papilloma virus (HPV). Unlabeled DNA probes have been immobilized by spontaneous coadsorption of thiolated single-stranded oligonucleotides (HS-ssDNA) onto the sensing surface of a screen-printed gold electrode (SPGE). The covalently immobilized single-stranded DNA probe (HS-ssDNA) could selectively hybridize with its complementary DNA (cDNA) in solution to form double-stranded DNA (dsDNA) on the surface. DNA is treated with acid (e.g., 0.5 M chloridric acid), and the acid-released purine bases are directly determined by square wave voltammetry (SWV).Variables of the probe-immobilization and hybridization steps are optimized to offer convenient quantitation of HPV DNA target, in connection with a short hybridization time. Peak currents were found to increase in the following order: hybrid-modified SPGE, 11-base mismatched modified SPGE, 18-base mismatched SPGE, and the probe modified SPGE. Control experiments with noncomplementary oligonucleotides were carried out to assess whether the suggested DNA sensor responds selectively to the target. The effect of the target DNA concentration on the hybridization signal was also studied. Under optimal conditions, this sensor has a good calibration range with HPV DNA sequence detection limit of 2 pg Á ml À1 (S=N ¼ 3).

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“…Fluorescein, the active component in the French preparation "fluoresceine," was quantitatively determined after gradient HPTLC development (95). Gradient mobile-phase TLC was also applied to the quantitative determination of prednisolone acetate in a Polish preparation "prednisolon" and in the aqueous humor of rabbit eyes (96).…”

Section: Gradient Elution In Analytical and Preparative Tlcmentioning

confidence: 99%

Gradient Development in Thin-Layer Chromatography

Gotkiewicz¹

2003

Handbook of Thin-Layer Chromatography

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Miniaturization of electroanalytical systems

Cited by 40 publications

References 21 publications

Poly(methylmethacrylate) and Topas capillary electrophoresis microchip performance with electrochemical detection

Poly(methylmethacrylate) and Topas capillary electrophoresis microchip performance with electrochemical detection

Label-Free DNA Biosensor for Electrochemical Detection of Short DNA Sequences Related to Human Papilloma Virus

Gradient Development in Thin-Layer Chromatography

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