Miniaturization of three carbohydrate analyses using a microsample plate reader

Convergent Evolution of Polysaccharide Debranching Defines a Common Mechanism for Starch Accumulation in Cyanobacteria and Plants

“…Polysaccharide was quantified in each 1-mL fraction by the phenolsulfuric acid method (Fox and Robyt, 1991). Polysaccharide fractions were pooled and then subjected to second size exclusion chromatography (Toyopearl TSK HW 55S).…”

“…Solution B consisted of 62 mg of copper sulfate pentahydrate and 63 mg of L-Ser in a total volume of 50 mL. The working reagent was freshly prepared by mixing the equal volume of Solutions A and B (Fox and Robyt, 1991). An amount of 225 mL of the sample was added to 225 mL of the working reagent in a tube, and the mixture was incubated at 80°C for 40 min in a water bath.…”

Effect of lactic acid and UV irradiation on the cassava and corn starches

“…The eluent consisted of NaCl (25 mM) and NaOH (1 mM) with a flow rate of 0.5 mL/min. Fractions of 2.5 mL each were collected and subjected to total carbohydrate and amylose content analysis using phenol sulfuric (Dubois et al, 1956) with modifications according to Fox and Robyt (1991), and iodine staining reactions (Juliano, 1971), respectively, to determine the molecular weight distribution of amylopectin and amylose.…”

Association of Novel Domain in Active Site of Archaic Hyperthermophilic Maltogenic Amylase from Staphylothermus marinus

“…IM6501 (14), neopullulanase from B. stearothermophilus (31), and T. vulgaris ␣-amylase II (13). Kinetic Parameters and Reaction Product Analysis-The copper bicinchoninate method was used to measure the concentration of reducing products to determine activity and kinetic parameters of SMMA (15,32). For thin layer chromatography (TLC) analysis, the reaction products were spotted onto Whatman K5F silica gel plates (Whatman plc, Maidstone, UK) and developed using isopropyl alcohol/ethyl acetate/water (3:1:1, v/v/v) as the solvent system.…”