Miniaturized Disposable Buckypaper-Polymer Substrate Based Electrochemical Purine Sensing Platform

Abstract

Herein, a novel, low-cost and disposable electrochemical sensor is presented by using recycled base material polyethyleneterephthalate (PET) obtained from a disposable drinking water bottle as a sensing platform. Bucky paper (BP) was used as a working electrode material. The CO2 laser was used for fabricating sensing substrate and the desired three electrode pattern of BP. This was attached on the PET substrate using a double sided tape. The lamination process was used to define the geometric area and for insulating the electrode. The determination of Xanthine (Xn) and Uric Acid (UA) were carried out as a proof of concept with unmodified BP surface. The electrochemical behavior of Xn and UA was studied using a cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometery (CA). The calibration curve for Xn and UA were obtained in the linear range 10 μM−500 μM and 50 μM−1000 μM respectively using and CA. The LOD obtained for Xn and UA was 6 μM and 22.5 μM respectively. Finally, the proposed method was applied for determination of Xn and UA in human serum sample with good selectivity and high sensitivity.