Miniaturized electrochemical sensor modified with aptamers for rapid norovirus detection

Wang, Nan; Kitajima, Masaaki; Mani, Kalaivani; Kanhere, Elgar; Whittle, Andrew J.; Triantafyllou, Michael S.; Miao, Jianmin

doi:10.1109/nems.2016.7758320

Cited by 8 publications

(7 citation statements)

References 6 publications

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“…The majority of aptamer‐based biosensor comprises foodborne pathogens, especially Salmonella and E. coli , even though there are different kinds of pathogens rather than foodborne ones. Besides, there were aptamer‐based biosensor studies based on detection of viruses, such as norovirus, 58–62 hepatitis B virus, 63,64 influenza virus, 65–70 and of course SARS‐CoV‐2 71,72 …”

Section: Electrochemical Aptasensors For Pathogenic Point‐of‐care Dia...mentioning

confidence: 99%

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Kızılkurtlu

Demirbaş

Ağel

2023

Biotech and App Biochem

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A biosensor system refers to a biomedical device, which detects biological, chemical, or biochemical components by converting those signals to an electrical signal by utilizing and uniting physical or chemical transducer with biorecognition elements. An electrochemical biosensor is generally based on the reaction of either production or consumption of electrons under a three‐electrode system. Biosensor systems are exploited in a wide range of areas, such as medicine, agriculture, husbandry, food, industry, environment protection, quality control, waste disposal, and the military. Pathogenic infections are the third leading cause of death worldwide after cardiovascular diseases and cancer. Therefore, there is an urgent need for effective diagnostic tools to control food, water, and soil contamination result in protecting human life and health. Aptamers are peptide or oligonucleotide‐based molecules that show very high affinity to their targets that are produced from large pools of random amino acid or oligonucleotide sequences. Generally, aptamers have been utilized for fundamental sciences and clinical implementations for their target‐specific affinity and have been intensely exploited for different kinds of biosensor applications for approximately 30 years. The convergence of aptamers with biosensor systems enabled the construction of voltammetric, amperometric, and impedimetric biosensors for the detection of specific pathogens. In this review, electrochemical aptamer biosensors were evaluated by discussing the definition, types, and production techniques of aptamers, the advantages of aptamers as a biological recognition element against their alternatives, and a wide range of aptasensor examples from literature in the detection of specific pathogens.

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Section: Electrochemical Aptasensors For Pathogenic Point‐of‐care Dia...mentioning

confidence: 99%

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Kızılkurtlu

Demirbaş

Ağel

2023

Biotech and App Biochem

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“…This biosensor was used to detect HuNoVs in lettuce with a LOD of 60 genomic copies/mL. Wang et al developed a miniature electrochemical biosensor on the basis of aptamer modification to detect HuNoV [90]. Murine norovirus (MNV) was used as an alternative model of HuNoVs.…”

Section: Electrochemical Biosensormentioning

confidence: 99%

Advances and Future Perspective on Detection Technology of Human Norovirus

et al. 2021

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Human norovirus (HuNoV) is a food-borne pathogen that causes acute gastroenteritis in people of all ages worldwide. However, no approved vaccines and antiviral drugs are available at present. Therefore, the development of accurate and rapid detection technologies is important in controlling the outbreak of HuNoVs. This paper reviewed the research progress on HuNoV detection, including immunological methods, molecular detection and biosensor technology. Immunological methods and molecular detection technologies are still widely used for HuNoV detection. Furthermore, biosensors will become an emerging developmental direction for the rapid detection of HuNoVs because of their high sensitivity, low cost, easy operation and suitability for onsite detection.

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“…These aptamers could also be used in combination with multiple sensing platforms for the detection of murine norovirus (used as a norovirus surrogate) or norovirus. One such example is the work by Wang and colleagues, where they combined aptamers with Micro-Electro-Mechanical Systems (MEMS) to develop a biosensor for norovirus [113].…”

Section: Emerging Detection Technologiesmentioning

confidence: 99%

“…A miniaturized and portable MEMS-based electrochemical aptasensor was developed and evaluated for the detection of norovirus [113]. The electrode surface was functionalized with virus-specific fluorescent aptamers using drop-casting methods.…”

Section: Emerging Detection Technologiesmentioning

confidence: 99%

Current and Emerging Technologies for the Detection of Norovirus from Shellfish

Gyawali

Beale

et al. 2019

Foods

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Reports of norovirus infections associated with the consumption of contaminated bivalve molluscan shellfish negatively impact both consumers and commercial shellfish operators. Current virus recovery and PCR detection methods can be expensive and time consuming. Due to the lack of rapid, user-friendly and onsite/infield methods, it has been difficult to establish an effective virus monitoring regime that is able to identify contamination points across the production line (i.e., farm-to-plate) to ensure shellfish quality. The focus of this review is to evaluate current norovirus detection methods and discuss emerging approaches. Recent advances in omics-based detection approaches have the potential to identify novel biomarkers that can be incorporated into rapid detection kits for onsite use. Furthermore, some omics techniques have the potential to simultaneously detect multiple enteric viruses that cause human disease. Other emerging technologies discussed include microfluidic, aptamer and biosensor-based detection methods developed to detect norovirus with high sensitivity from a simple matrix. Many of these approaches have the potential to be developed as user-friendly onsite detection kits with minimal costs. However, more collaborative efforts on research and development will be required to commercialize such products. Once developed, these emerging technologies could provide a way forward that minimizes public health risks associated with shellfish consumption.

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Miniaturized electrochemical sensor modified with aptamers for rapid norovirus detection

Cited by 8 publications

References 6 publications

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Electrochemical aptasensors for pathogenic detection toward point‐of‐care diagnostics

Advances and Future Perspective on Detection Technology of Human Norovirus

Current and Emerging Technologies for the Detection of Norovirus from Shellfish

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