Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

Abstract

An experiment for strawberry Fragaria ananassa cv. Festival in vitro conservation of cultures for nine months was studied. Three MS medium strength (full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9 months) in slowing down or reducing growth. Results showed a significant effect of the medium salt strength, as the full strength treatment was higher by achieved the highest percentage of viability (survival %) and storage characteristics of cultures (N%, P%, carbohydrates%, protein %) and regeneration ability characteristics (shoots and roots number and length). Three-month storage period achieved the highest rate compared to the nine-month storage period, which recorded the lowest average for the studied characteristics. The full strength treatment for three months storage period realized the highest rate compared to treatment ¼ strength for the storage period of nine months, which recorded the lowest rate of the studied traits. The application of minimal strength for in vitro conservation allows strawberry cultures to remain viable with a slow growth rate and a significant increase in the storage life of the tissues to nine months.

Effect of Explants Type and Plant Growth Regulators on the Stimulation and Multiplication of Vegetative Branches of Trigonella foenum-graecum L. in Vitro

“…The rest of the plant parts were immersed in a 2% sodium hypochlorite solution for 15 minutes to disinfect them superficially in sterile conditions, and finally they were washed three times in distilled water for an average of 5 minutes each time. 1 time to remove the traces of the sterile material [14]. the seeds were sown in sterile laboratory culture bottles equipped with MS liquid media, free of growth regulators.…”

Propagation of Date Palm (Phoenix dactylifera L.) Cultivar Jawzi Using Shoot Tip

“…The shoots were then allowed to grow and develop roots for a period of three months. Following the rooting stage, the shoots that had been rooted were subsequently transferred to a halfstrength liquid MS medium for an additional duration of one month, in order to undergo a pre-acclimatization stage (Kadhim & Abdulhussein, 2021). During the entire process, the plantlets on solid medium and those in the culture media were incubated at a temperature of 25 ± 2 °C, with a light intensity of 2000 lux in a 16/8 light/dark cycle.…”