Minimal tumour burden in haematological diseases: a step forward with quantitative assessment of Bence‐Jones in nephelometry?

Pieri, Massimo; Stefano, Alessandro De; Franceschini, Luca; Rizzo, Manuela; Duranti, Fabio; Bernardini, Sergio; Zenobi, Rossella; Dessı̀, Mariarita

doi:10.1111/bjh.13878

Cited by 4 publications

(1 citation statement)

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“…5 It is also important during follow-up to evaluate a patient’s response to therapy and as supportive evidence for minimal tumor burden. 6 According to guidelines published in 2003, 5 the gold standard method for the identification and characterization of BJP is immunofixation electrophoresis of urinary proteins, since it both detects the presence of FLCs and determines if they are monoclonal or polyclonal. After electrophoresis, the BJP peak can be quantified by densitometry.…”

Section: Introductionmentioning

confidence: 99%

κ and λ urine free light chains: a new method for quantification

Giussani

Ciniselli

Macciotta

et al. 2020

Tumori

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Background: Immunofixation electrophoresis of urinary proteins, coupled with densitometric analysis, is the gold standard method for determining urinary monoclonal free light chains (FLCs), i.e. Bence Jones protein. Recently, immunochemical methods have been developed for Bence Jones protein quantification, but no such method has been widely adopted. This study evaluated a new antibody-based immunoturbidimetry method for urinary FLC quantification, using immunofixation electrophoresis as reference. Methods: κ and λ FLCs were measured in urine specimens from 95 (training cohort) and 103 (testing cohort) patients by both immunofixation electrophoresis and immunoturbidimetry. Results: There was almost perfect concordance in the training cohort between the new immunoturbidimetry assay and immunofixation electrophoresis and substantial agreement, with Cohen kappa of 0.85 and 0.75, for κ and λ FLC determination, respectively. Results were confirmed in the testing cohort, where Cohen kappa was 0.86 for κ and 0.94 for λ FLCs. The κ FLC assay had 88% sensitivity and 98%–100% specificity; the λ FLC assay had 94% and 96% sensitivity and 91% and 99% specificity in the training and testing cohorts, respectively. Conclusions: The new immunochemical method has a satisfactory performance and almost perfect agreement with immunofixation electrophoresis and gives the advantage of FLC quantification.

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Section: Introductionmentioning

confidence: 99%