“…Chronic infection presents with progressive pain, skin fistula formation, and/or drainage of purulent secretions, and occurs >3 months after the intervention of arthroplasty (dissemination of the infection can be hematogenous, with a primary starting point, or by direct inoculation in the case of infectious agents with low virulence, especially in the case of coagulase-negative staphylococci, Cutibacterium acnes , and Corynebacterium). In order to diagnose PJI, the histology of the synovial tissue, the number of leukocytes in the synovial fluid [ 4 ], determination of the levels of systemic inflammatory markers (such as ESR, CRP, and IL-6 [ 9 ]), imaging investigations (such as standard radiographs), technetium-labeled bone scans, MRI, CT, and PET/CT have been used [ 10 ]. Recently, new techniques have been developed to identify the pathogen, the conventional methods used in the past were accompanied by a high failure rate (due to the lack of isolation of a pathogenic microorganism through the premature administration of antibiotics and seeding techniques on culture media) [ 11 ].…”