Minimizing inhibition of PCR-STR typing using digital agarose droplet microfluidics

Geng, Tao; Mathies, Richard A.

doi:10.1016/j.fsigen.2014.10.007

Cited by 20 publications

(10 citation statements)

References 36 publications

(43 reference statements)

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“…Due to mitigation of inhibitory effects achieved by efficient removal of inhibitors from agarose microgel droplets, this strategy enhanced the tolerance of tannic acid, humic acid, and urea, respectively (∼10-fold, ∼16-fold, and ∼20-fold), in STR typing of GM09948 human lymphoid cells. The portable device with superior performance suggests that this digital agarose microfluidic strategy is a promising approach for forensic analysis at the crime scene …”

Section: Other Applications Of Natsmentioning

confidence: 99%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are natural biopolymers composed of nucleotides that store, transmit, and express genetic information. Overexpressed or underexpressed as well as mutated nucleic acids have been implicated in many diseases. Therefore, nucleic acid tests (NATs) are extremely important. Inspired by intracellular DNA replication and RNA transcription, in vitro NATs have been extensively developed to improve the detection specificity, sensitivity, and simplicity. The principles of NATs can be in general classified into three categories: nucleic acid hybridization, thermal-cycle or isothermal amplification, and signal amplification. Driven by pressing needs in clinical diagnosis and prevention of infectious diseases, NATs have evolved to be a rapidly advancing field. During the past ten years, an explosive increase of research interest in both basic research and clinical translation has been witnessed. In this review, we aim to provide comprehensive coverage of the progress to analyze nucleic acids, use nucleic acids as recognition probes, construct detection devices based on nucleic acids, and utilize nucleic acids in clinical diagnosis and other important fields. We also discuss the new frontiers in the field and the challenges to be addressed.

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Section: Other Applications Of Natsmentioning

confidence: 99%

Nucleic Acid Tests for Clinical Translation

et al. 2021

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“…Another approach for recovering individual cells is the DEPArray system, which is an image-based, microfluidic digital sorter that can isolate pure cells (Fontana et al 2017, Williamson et al 2018. DNA profile recovery can also be improved through separating PCR inhibitors and DNA templates using a digital agarose ______________________________________________________________________________________________________ This publication is available free of charge from: https://doi.org/10.6028/NIST.IR.8351-draft droplet microfluidic approach (Geng et al 2015). Similarly, agarose reactors can also allow for single-cell PCR within an encapsulated droplet (Geng et al 2014).…”

Section: ______________________________________________________________________________________________________mentioning

confidence: 99%

(Draft) DNA Mixture Interpretation

Butler¹

2021

Preprint

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The initial release of this report is a draft document, and we welcome comments and feedback from readers. All relevant submitted comments will be made publicly available and will be considered when finalizing this report. Do not include personal information, such as account numbers or Social Security numbers, or names of other individuals. Do not submit confidential business information, or otherwise proprietary, sensitive, or protected information. We will not post or consider comments that contain profanity, vulgarity, threats, or other inappropriate language or like content. During the 60-day comment period, comments may be sent to scientificfoundationreviews@nist.gov. All comments, including commenter name and affiliation, will be published at https://www.nist.gov/dna-mixture-interpretation-nist-scientific-foundation-review. Certain commercial entities, equipment, or materials may be identified in this document in order to describe an experimental procedure or concept adequately. Such identification is not intended to imply recommendation or endorsement by the authors or the National Institute of Standards and Technology, nor is it intended to imply that the entities, materials, or equipment are necessarily the best available for the purpose.

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“…DMF technologies update this procedure like we have already discussed in this paper. Digital PCR can provide a robustness and convenience which can mitigate the effects of PCR inhibitors in diluted samples, regardless of the form of template (i.e., cells or genomic DNA) for forensic short tandem repeat (STR) typing at the single-cell/molecule level [141].…”

Section: Droplet-based Microfluidics For the Field Applicationsmentioning

confidence: 99%

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

2019

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Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.

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Minimizing inhibition of PCR-STR typing using digital agarose droplet microfluidics

Cited by 20 publications

References 36 publications

Nucleic Acid Tests for Clinical Translation

Nucleic Acid Tests for Clinical Translation

(Draft) DNA Mixture Interpretation

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

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