Minimizing UV noise in supercritical fluid chromatography. I. Improving back pressure regulator pressure noise

Instrumentation for analytical scale supercritical fluid chromatography

“…As with post column temperature optimization, any pressure noise induced by the BPR will result in RI variations in any UV-Vis detector flow cell, and detector noise [14]. Some modern BPR's can control back pressure to <±0.05 bar (0.75 psi) at 200 bar, resulting in remarkably low pressure induced noise.…”

Maximizing kinetic performance in supercritical fluid chromatography using state-of-the-art instruments

“…To overcome this weakness, two of the most important providers of chromatographic instrumentation (Agilent and Waters) developed a new generation of analytical SFC instruments, allowing comparable sensitivity and robustness to that of HPLC [10][11][12]. In parallel, polar stationary phases packed with core-shell sub-3 m particles [13] or fully porous sub-2 m particles [14][15][16][17] compatible with supercritical conditions have been commercialized, enabling a user to achieve kinetic performance comparable and sometimes even better than in LC.…”

Supercritical fluid chromatography for GMP analysis in support of pharmaceutical development and manufacturing activities

“…Customized instrumentation allowed many of these shortcomings to be overcome [18,19] but required expert solutions. Consequently, many laboratories carrying out GMP testing of stereoisomeric mixtures currently use traditional normal phase or reversed phase chiral chromatographic methods.…”