Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans

Vasconcelos, Laurylene César de Souza; Sampaio, Fábio Correia; Sampaio, Maria Carmélia Correia; Pereira, Maria do Socorro Vieira; Higino, Jane Sheila; Peixoto, Maria Helena Pereira

doi:10.1590/s0103-64402006000300009

Cited by 229 publications

(122 citation statements)

References 17 publications

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“…Tannins also carry out this activity by precipitating the cell wall and cell membrane. [14][15] Antimicrobial activity can also be due to the suppression of glycosyltransferase. 15,16 Previous researches have also demonstrated the antibacterial effect of gallic acid at low concentrations against tested sensitive strains.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 P. granatum extract was used to regulate the adherence of various microorganisms present in the oral cavity and it was effective against the various species like S. mutans, S. mitis and C. albicans. 14 It has also been demonstrated to reduce carcinogenic bacterial metabolites responsible for tooth decay by using the peel extract of P. granatum. 18 Our study also supports the earlier findings that P. granatum is very rich in antimicrobial constituents and reiterates further research on the purification and characterization of various compounds in addition to their mode of action.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Antimicrobial Property of Extract of Punica Granatum (L.) on Oral Pathogens

K¹,

2022

Int J Life Sci Pharm Res

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The aim of the present research was the examination of the potential activity of various organic solvent extracts of Punica granatum on the important microbiota harbouring in the oral cavity. The dried peels and seeds of P. granatum were extracted using chloroform, methanol and nbutanol and their potential activity were studied. The inhibitory effect of crude extracts were observed on various microbial plates which were incubated with different bacterial species in terms of zone of inhibition as mm in diameter. The data was analysed by One Way Analysis of Variance for significance. The peel extract was more potent in inhibiting the microbial population growth as compared to seed extract. While all the four solvents were effective in case of peel extracts, the bioactivity was much higher in species such as Streptococcus mutants, Streptococcus sanguis, Staphylococcus aureus, Lactobacillus acidophilus and Actinomyces viscosus, among 8 species evaluated in the present investigation. The peel extract of P. granatum contains active biomolecule(s) against important oral microbial pathogens and thus holds promise as a potential bioactive molecule against Dental Caries. Based on the bioactivity analysis, the peel extract of P. granatum may further be subjected to purification and characterization of the bioactive compound(s) for regulating oral microbial contamination.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Antimicrobial Property of Extract of Punica Granatum (L.) on Oral Pathogens

K¹,

2022

Int J Life Sci Pharm Res

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“…Antitumour (Afaq et al, 2005;Lansky and Newman, 2007), antibacterial (Prashanth et al, 2001;Braga et al, 2005;Menezes et al, 2006;Aqil, Ahmad, 2007), antidiarrhoeal (Das et al, 1999;Mathabe et al, 2006), antifungal (Dutta et al, 1998Vasconcelos et al, 2003;Vasconcelos et al, 2006), antiulcer (Gharzouli et al, 1999), and antioxidant pharmacological properties have been reported for various extracts/constituents of different parts of this plant species. Recently, the antioxidant activity of P. granatum associated with its phytochemicals, such as, polyphenols, flavonoids, and anthocyanidins has gained importance (Faria et al, 2007;Guo et al, 2007;Kulkarni et al, 2007;Mirdehghan et al, 2007;Reddy et al, 2007;Rout, Banerjee, 2007;Sestili et al, 2007;Zaid et al, 2007).…”

Section: Introductionmentioning

confidence: 97%

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Valadares

Pereira

Benfica

et al. 2010

Braz. J. Pharm. Sci.

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In the present study, the ability of Punica granatum ethanolic leaf extract (PGL) and Punica granatum ethanolic fruit extract (PGF) to induce mutagenicity or to modulate the genotoxic effects induced by the alkylating agent cyclophosphamide (CP) was evaluated. Swiss male mice were treated by gavage for 10 days with PGL or PGF (12.5, 25, 50, and 75 mg/kg/day) prior to exposure to CP (i.p. 200 mg/kg), 24 h after the end of the treatment. Initial observations revealed that normal mice treated with both extracts (12.5, 25, 50, and 75 mg/kg/day) showed a similar micronucleated polychromatic erythrocyte (MNPCE) frequency to that of the control group. Investigation of the protective effect of PGL and PGF based on data analysis revealed that, irrespective of dose or extract, oral administration of PGL or PGF for 10 days prior to exposure had reduced, in a dose-dependent manner, the frequency of MNPCE induced by CP in all groups studied. Higher reductions were observed at PGF doses of 50 and 75 mg/kg. Taken together, these results demonstrate that mice treated with P. granatum showed an absence of mutagenic effects and dose-dependent protective effects against CP-induced oxidative DNA damage. Uniterms:Punica granatum/mutagenic effects. Punica granatum/antimutagenic effects. Micronucleic. Genotoxicity. Cyclophosphamide.No presente estudo investigamos o potencial do extrato etanólico das folhas da Punica granatum (PGFO) e do extrato etanólico dos frutos da Punica granatum (PGFR) de induzir mutagenicidade ou de proteger contra efeitos genotóxicos induzidos pela ciclofosfamida (CF). Camundongos machos Swiss foram tratados por 10 dias, via oral, com PGFO ou PGFR (12,5, 25, 50 e 75 mg/kg/dia), previamente a exposição à CF (i.p. 200 mg/kg) 24 horas após término do tratamento. Observamos que os animais tratados por 10 dias com ambos os extratos (12,5, 25, 50 e 75 mg/kg/dia) demonstraram a frequência de micronúcleo policromático eritrocitário (MNPCE) similar ao grupo controle. Quando aos efeitos protetores dos extratos foram investigados, a análise dos dados revelou que, independentemente da dose ou do extrato usado, a administração oral por 10 dias, previamente à exposição, reduziu, de forma dose-dependente, a frequência de MNPCE induzidos pela CF, em todos os grupos estudados. As maiores reduções foram observadas com PGFR nas doses de 50 e 75 mg/kg. Em conjunto, sob as condições testadas, camundongos tratados com P. granatum demonstraram ausência de efeitos mutagênicos e, de forma dose-dependente, efeitos protetores contra os danos oxidativos do DNA induzidos pela CF. Unitermos:Punica granatum/efeitos mutagênicos. Punica granatum/efeitos antimutagênicos. Micronúcleo. Genotoxicidade. Ciclofosfamida. INTRODUCTIONPunica granatum Linn., a Punicaceae, commonly known as pomegranate, has a vast ethnomedical history and represents a phytochemical reservoir of heuristic medicinal value (Lansky and Newman, 2007). The plant as a whole and especially its flowers, have been extensively used in the ancient Ayurvedic health care system (Siv...

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“…These include anti-inflammatory, antitumoral, diabetes prevention/ treatment, antiulcer, antimicrobial, antiangiogenic, immunomodulating and antioxidant activities. The medicinal properties of this fruit have been widely investigated and numerous scientific papers have corroborated with its popular use (Prashanth et al, 2001;Braga et al, 2005;Menezes et al, 2006;Vasconcelos et al, 2006;Aquil, Ahmad, 2007;Oliveira et al, 2010;Johanningsmeier, Harris, 2011).Analyses of the antioxidant property of pomegranate have shown that this fruit is superior to other foods in terms of antioxidant effects, such as red wine, cranberries and green tea (Seeram et al, 2008). Moreover certain reports have shown considerable antioxidant activities of Punica granatum in several in vitro and in vivo experimental models (Chidambara et al, 2002;Cerda et al, 2003;Ricci et al, 2006;Bishayee et al, 2011).…”

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confidence: 95%

Punica granatum L. protects mice against hexavalent chromium-induced genotoxicity

Ávila

Guerra

Borges

et al. 2013

Braz. J. Pharm. Sci.

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This study investigated the chemoprotective effects of Punica granatum L. (Punicaceae) fruits alcoholic extract (PGE) on mice exposed to hexavalent chromium [Cr(VI)]. Animals were pretreated with PGE (25, 50 or 75 mg/kg/day) for 10 days and subsequently exposed to a sub-lethal dose of Cr(VI) (30 mg/kg). The frequency of micronucleated polychromatic erythrocytes in the bone marrow was investigated and the Cr(VI) levels were measured in the kidneys, liver and plasm. For the survival analysis, mice were previously treated with PGE for 10 days and exposed to a single lethal dose of Cr(VI) (50 mg/kg). Exposure to a sub-lethal dose of Cr(VI) induced a significant increase in the frequency of micronucleated cells. However, the prophylactic treatment with PGE led to a reduction of 44.5% (25 mg/kg), 86.3% (50 mg/kg) and 64.2% (75 mg/kg) in the incidence of micronuclei. In addition, the 50 mg/kg dose of PGE produced a higher chemoprotective effect, since the survival rate was 90%, when compared to that of the non-treated group. In these animals, reduced amounts of chromium were detected in the biological materials, in comparison with the other groups. Taken together, the results demonstrated that PGE exerts a protective effect against Cr(VI)-induced genotoxicity. Uniterms INTRODUCTIONChromium occurs naturally in the environment in different oxidation states, where Cr(III) and Cr(VI) are the most prevalent. The hexavalent form is usually linked with oxygen and is a strong oxidizing agent. Studies have shown that Cr(VI) is a mutagenic, carcinogenic and teratogenic agent, which easily penetrates the cell membrane in the form of chromate anion (Barceloux, 1999;Acharya et al., 2006;Nickens et al., 2010;Chang et al., 2011;Okello et al., 2012). Its molecular mechanisms of toxicity are not yet fully known, but it is believed that the intracellular increase in the production of reactive oxygen species (ROS) is probably a key factor in inducing cell damage (Ye et al., 1999;Wu et al., 2012). These reactive species can cause damage to macromolecules such as DNA, proteins and lipids, thereby inhibiting their functions (Valko et al., 2007;Yan et al., 2012) and may even trigger apoptotic cell death (Liu, Chen, 2006;Liu et al., 2008).Cr(VI) has been widely used in metallurgical, refractory and chemical industries. It has been considered a potent occupational carcinogen among workers involved in electrodeposition, metal finishes/welding, wood preservatives, organic synthesis procedures and in leather tanning (Barceloux, 1999;Shrivastava et al., 2002;Acharya et al., 2006;Nickens et al., 2010). In such activities occupational exposure to Cr(VI)-contaminated dust and aerosols occurs predominantly through inhalation or skin contact (Zagrodzki et al., 2007). Non-occupational exposure to Cr(VI) can also occur through cigarette smoke, automobile emissions and the inappropriate disposal of chromium-containing waste in the environment (O'Brien et al., 2003;Russo et al., 2005).The literature has shown that the administration of antioxidant agents ...

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Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans

Cited by 229 publications

References 17 publications

Evaluation of Antimicrobial Property of Extract of Punica Granatum (L.) on Oral Pathogens

Evaluation of Antimicrobial Property of Extract of Punica Granatum (L.) on Oral Pathogens

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Punica granatum L. protects mice against hexavalent chromium-induced genotoxicity

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