Minimum Inhibitory Concentration of Probiotic Soy-Milk Yoghurt (SOYGHURT) Towards Porphyromonas gingivalis [IN VITRO]

Abstract

Abstract-The main cause of chronic periodontitis isPorphyromonas gingivalis that can be found in subgingival plaque. Porphyromonas gingivalis can be inhibited by probiotic product such as yoghurt. Yoghurt is a beverage from milk that proceed by fermentation with adding lactic acid bacteria. Yoghurt is usually made of from cow's milk, but in this study are been made by soymilk. The aim of this study is to know the inhibition effect of yoghurt probiotic to Porphyromonas gingivalis growth. Soyghurt is made with adding lactic acid bacteria with 2%, 3%, 4%, and 5% concentration. The inhibition test of this probiotic is done with an agar-well diffusion assay. The result was analyzed with one way ANOVA and LSD test. The result of this study shows that there's difference of inhibition zone of Porphyromonas gingivalis among 2%, 3%, 4%, and 5% (p<0,05). The result of this study shows that the widest inhibition zone are in 4% concentrate (14,63 mm) and 5% concentrate one (17,53 mm) which are in weak group based on Ahn classification. The conclusion of this study is the is soyghurt has an inhibition effect of the growth of Porphyromonas gingivalis in 4% and 5% concentrate.

Nanoparticles of Liquid Smoke Rice Husk Inhibit Porphyromonas gingivalis

“…17 The polar form of protons interacts with phenol to form a weak hydrogen bond that can cause changes in protein structure that have a role in the process of cell metabolism with enzymes in the catalytic process in bacteria and cause an imbalance in the formation of proteins in cells so that bacteria will die slowly. 2 18 19 20…”

“…1 Periodontitis that often occurs is chronic periodontitis, often due to colonization by Porphyromonas gingivalis with a prevalence rate of ∼80.5% cases. 2 Periodontitis treatment performed includes controlling the accumulation of plaque in the oral cavity and also root canal treatment to eliminate bacteria that cause infection 3 Administration of antibiotics is used as a support in reducing the number of colonies of Porphyromonas gingivalis, but it should be noted that antibiotics can cause side effects that can reduce drug concentrations in target cells and a resistance to bacteria due to improper use and not as recommended, it will cause bacteria to grow, easily adaptable and immune to antibiotics. 4…”