The definition of experimental plot size is an essential tool to ensure precision in statistical analysis in experiments. The objective of this study was to estimate the plot size for the cactus pear cv. Gigante using the Modified Maximum Curvature Method, under the semi-arid conditions of Northeastern Brazil. The uniformity test was conducted at the Federal Institute of Bahia, Guanambi Campus, Bahia state, Brazil, during the agricultural period from 2009 to 2011. The spatial arrangement was composed of ten rows with 50 plants each, whose evaluated area was formed by the eight central rows with 48 plants per row, making 384 plants and area of 153.60 m2. The following variables were evaluated: plant height; length, width and thickness of cladode; number of cladodes; total area of cladodes; cladode area and green mass yield in the third production cycle. In the evaluations, each plant was considered as a basic experimental unit (BEU), with an area of 0.4 m2, comprising 384 basic units (BU), whose adjacent ones were combined to form 15 pre-established plot sizes with rectangular shapes and in rows. The characteristics total area of cladodes and green mass yield require larger plot sizes to be evaluated with greater experimental accuracy. For experimental evaluation of cactus pear cv. Gigante, plot size should be eight plants in the direction of the crop row.