Minimum sizes for remnant and transplanted livers in rats

Abstract

Abstract:Our new technique of hepatectomy in rats, avoiding inferior vena cava constriction, revealed that 5%-10% of the total liver is the minimum required volume for remnant liver, a smaller amount than previously reported. The characteristic histological findings of remnant liver in perioperative death are marked hepatocytosis and hepatocyte apoptosis, and extensive hepatocyte necrosis with sinusoid congestion, all of which are thought to be caused by hyperendotoxemia and mitochondrial energy crisis. Partial liver transplantation in rats with our new technique of hepatectomy in the donor operation showed that a graft of 20% liver was the minimum required volume for successful transplantation, also a smaller amount than previously reported. There was no difference in the rate of increase in wet weight after surgery between 20% remnant liver and 20% transplanted liver. Differences between 10% minimum volume remnant liver and 20% minimum transplanted liver appeared to be caused by ischemia-reperfusion and cold preservation injury to the transplanted liver.

Non-toxicity of IV Injected Perfluorocarbon Oxygen Carrier in an Animal Model of Liver Regeneration Following Surgical Injury

“…to a liver/BW ratio of 3.5, is 7-8 days. This duration is in agreement with prior experience with this surgical model [18][19][20][21][22][23].…”

“…As per the protocol first described by Higgins and Andersen in 1931 [18][19][20][21][22][23], 3/4 liver resections were carried out (on day 0) on one half (N = 24) of the animals. Briefly, while the animals were under isoflurane anesthesia, a midline incision was made, followed by complete liberation of all liver ligaments to allow the ligation of the pedicles of the median and left lateral lobes i.e.…”

“…This model emulates a seriously compromised liver, with failure in the beginning followed by progressive regeneration. Several previous studies have established that 100 % of such animals will recover [18][19][20][21][22][23]. Thus, the purpose of these experiments was, by using an animal model, to investigate the effects of IV administered PFOB on the recovery of a liver failure patient.…”

Gene expression of ATP-binding cassette transporters during liver regeneration after 90% hepatectomy in rats

“…The hepatic arteries and the portal veins were dissected and the abdomen was then closed. ii) the 90% partial hepatectomy group (n=24); a 90% partial hepatectomy was performed according to a new technique described by Kubota et al (24), which was modified from the original procedure established by Higgins and Anderson (25). It was reported that all of the 90% hepatectomy rats survived using this technique (17,26).…”

Rodent Models and Imaging Techniques to Study Liver Regeneration

“…Using this technique for 90% PHx carries a high risk of causing outflow obstruction or massive bleeding. (2) The vessel-oriented technique, which Kubota et al [47] introduced in 1997. In their study, ligation of the portal vein and hepatic artery was performed prior to ligation of the respective hepatic vein with a piercing suture, and then, the individual liver lobe was resected without the risk of bleeding and compressing the inferior vena cava.…”