Mining, Analyzing, and Evolving Data-Intensive Software Ecosystems

Nagy, Csaba; Lanza, Michele; Cleve, Anthony

doi:10.1007/978-3-031-36060-2_11

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2024

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“…FxM images were analyzed using a custom pipeline ( Figure 1—figure supplement 1A ) implemented in the Julia language ( Bezanson et al, 2017 ) available on GitHub (copy archived at Nagy, 2024b ). Briefly, the raw images were denoised with a patch-based algorithm ( Boulanger et al, 2010 ) and then the edges were enhanced using a Scharr kernel.…”

Section: Methodsmentioning

confidence: 99%

Neutrophils actively swell to potentiate rapid migration

Nagy,

Strickland,

Weiner

2024

eLife

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While the involvement of actin polymerization in membrane protrusion is well-established, we have a more limited understanding of the role of transmembrane water flow in cell motility. Here we investigate the role of water influx in neutrophil migration. These cells undergo directed movement to sites of injury and infection. Chemoattractant exposure increases cell volume and potentiates neutrophil migration, but the causal link between these processes is not known. Using a genome-wide CRISPR screen, we identify the regulators of the chemoattractant-induced neutrophil swelling, including NHE1, AE2, PI3K-gamma, and CA2. Through NHE1 inhibition in primary human neutrophils, we show that cell swelling is both necessary and sufficient for rapid migration following chemoattractant stimulation. Our data demonstrate that cell swelling complements cytoskeletal inputs for chemoattractant-induced potentiation of migration.

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Section: Methodsmentioning

confidence: 99%