Mining of Minor Disease Resistance Genes in V. vinifera Grapes Based on Transcriptome

Abstract

Intraspecific recurrent selection in V. vinifera is an effective method for grape breeding with high quality and disease resistance. The core theory of this method is the substitution accumulation of multi-genes with low disease resistance. The discovery of multi-genes for disease resistance in V. vinifera may provide a molecular basis for breeding for disease resistance in V. vinifera. In this study, resistance to downy mildew was identified, and genetic analysis was carried out in the interspecific crossing population of V. vinifera (Ecolly × Dunkelfelder) to screen immune, highly resistant and disease-resistant plant samples; transcriptome sequencing and differential expression analysis were performed using high-throughput sequencing. The results showed that there were 547 differential genes (194 up-regulated and 352 down-regulated) between the immune group and the highly resistant group, and 103 differential genes (54 up-regulated and 49 down-regulated) between the highly resistant group and the highly resistant group, there were 199 differential genes (50 up-regulated and 149 down-regulated) between the two groups. KEGG analysis of differentially expressed genes in the immune versus high-resistance group. The pathway is mainly concentrated in phenylpropanoid biosynthesis, starch and sucrose metabolism, MAPK signaling pathway–plant, carotenoid biosynthesis and isoquinoline alkaloid biosynthesis. The differential gene functions of immune and middle-resistant, high-and middle-resistant combinations were mainly enriched in plant–pathogen interaction pathway. Through the analysis of disease resistance-related genes in each pathway, the potential minor resistance genes in V. vinifera were mined, and the accumulation of minor resistance genes was analyzed from the molecular level.