Mining the landfill soil metagenome for denitrifying methanotrophic taxa and validation of methane oxidation in microcosm

Singh, Ashish Kumar; Nakhate, Suraj Prabhakarrao; Gupta, Rakesh Kumar; Chavan, Atul Rajkumar; Poddar, Bhagyashri Jagdishprasad; Prakash, Om; Shouche, Yogesh S.; Purohit, Hemant J.; Khardenavis, Anshuman A.

doi:10.1016/j.envres.2022.114199

Cited by 5 publications

(3 citation statements)

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“…Abbreviated references have been provided and the raw input data used to generate this figure can be found in Supplementary Data 4 on the Open Science Framework (OSF) at https://doi.org/10.17605/OSF.IO/ 6X5ZC 37 . Data obtained from (in chronological order): 8,15,16,[18][19][20]22,23,35,36,68,[117][118][119][120][121][122] . Source Data are provided as a Source Data file.…”

Section: Methanogen Families Occurring In Landfillsmentioning

confidence: 99%

“…The detection of two Methylacidiphilaceae populations in cells B and C is of note because, outside of two sequencing studies that detected this family in a corroding sewer pipe 74 and landfill soils 36 , the Methylacidiphilaceae have been largely characterized in acidic geothermal habitats (compiled in 75 ). Phylogenomic analyses show that MAGs STB_62 and STC_89 are distinct from each other and clustered within the Methylacidimicrobium genus, which is considered to be a "mesophilic" clade within the Methylacidiphilaceae [75][76][77][78].…”

Section: Complex IV High Affinitymentioning

confidence: 99%

“…Genome-resolved metagenomics 34 can be used to characterize microbial diversity across physicochemical gradients, including identifying factors constraining the distribution of methanogens in MSW 35 and the range of methanotrophic lifestyles that can be supported (e.g., facultative vs. obligate, aerobic vs. microaerophilic vs. anaerobic). Genome-resolved metagenomics also provides the opportunity to consider the contributions of methane-cycling organisms in the broader context of nutrient-cycling pathways and central redox metabolisms that control landfill biogeochemistry 36 . Although amplicon sequencing surveys have shed light on the changes in community structure that occur across landfill habitats, metagenomic surveys that examine the major guilds and physiological pathways controlling biogeochemical cycles over the spatial and temporal scales relevant to landfill lifecycles are lacking.…”

mentioning

confidence: 99%

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Microbial methane cycling in a landfill on a decadal time scale

Grégoire,

George,

Hug

2023

Nat Commun

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Landfills generate outsized environmental footprints due to microbial degradation of organic matter in municipal solid waste, which produces the potent greenhouse gas methane. With global solid waste production predicted to increase substantially in the next few decades, there is a pressing need to better understand the temporal dynamics of biogeochemical processes that control methane cycling in landfills. Here, we use metagenomic approaches to characterize microbial methane cycling in waste that was landfilled over 39 years. Our analyses indicate that newer waste supports more diverse communities with similar composition compared to older waste, which contains lower diversity and more varied communities. Older waste contains primarily autotrophic organisms with versatile redox metabolisms, whereas newer waste is dominated by anaerobic fermenters. Methane-producing microbes are more abundant, diverse, and metabolically versatile in new waste compared to old waste. Our findings indicate that predictive models for methane emission in landfills overlook methane oxidation in the absence of oxygen, as well as certain microbial lineages that can potentially contribute to methane sinks in diverse habitats.

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