MinION sequencing to genotype US strains of infectious laryngotracheitis virus

Spatz, Stephen J.; Garcı́a, Maricarmen; Riblet, Sylva M.; Ross, Teresa A.; Volkening, Jeremy D.; Taylor, Tonya L.; Kim, Taejoong; Afonso, Claudio L.

doi:10.1080/03079457.2019.1579298

Cited by 19 publications

(23 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MinIon sequencing was also used as diagnostic tool in USA to genotype the different ILTV isolates. Full genome (n ¼ 27) of ILTV were analyzed and it was identified to have 9 genotypes which can be grouped into 5 genotypes based on single allele assay using MinIon (Spatz et al 2019).…”

Section: Diagnosismentioning

confidence: 99%

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Gowthaman

Kumar

Koul

et al. 2020

Veterinary Quarterly

View full text Add to dashboard Cite

Infectious laryngotracheitis (ILT) is a highly contagious upper respiratory tract disease of chicken caused by a Gallid herpesvirus 1 (GaHV-1) belonging to the genus Iltovirus, and subfamily Alphaherpesvirinae within Herpesviridae family. The disease is characterized by conjunctivitis, sinusitis, oculo-nasal discharge, respiratory distress, bloody mucus, swollen orbital sinuses, high morbidity, considerable mortality and decreased egg production. It is well established in highly dense poultry producing areas of the world due to characteristic latency and carrier status of the virus. Co-infections with other respiratory pathogens and environmental factors adversely affect the respiratory system and prolong the course of the disease. Latently infected chickens are the primary source of ILT virus (ILTV) outbreaks irrespective of vaccination. Apart from conventional diagnostic methods including isolation and identification of ILTV, serological detection, advanced biotechnological tools such as PCR, quantitative real-time PCR, next generation sequencing, and others are being used in accurate diagnosis and epidemiological studies of ILTV. Vaccination is followed with the use of conventional vaccines including modified live attenuated ILTV vaccines, and advanced recombinant vector vaccines expressing different ILTV glycoproteins, but still these candidates frequently fail to reduce challenge virus shedding. Some herbal components have proved to be beneficial in reducing the severity of the clinical disease. The present review discusses ILT with respect to its current status, virus characteristics, epidemiology, transmission, pathobiology, and advances in diagnosis, vaccination and control strategies to counter this important disease of poultry.

show abstract

Section: Diagnosismentioning

confidence: 99%

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Gowthaman

Kumar

Koul

et al. 2020

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…The genetic distance had been found between predominant wild ILTV strains isolated from the vaccinated commercial layer and the vaccine strain WG in China. According to Agnew-Crumpton's and Choi's classification method [10,26,27], the results showed that predominant wild ILTV strains were close to class 8 by phylogenetic analysis of TK, ICP4, ORFB-TK, and the gB gene, while WG strain belonged to class 4 (Table 1), which indicated that the update of the vaccine was necessary because the ILTV virus was constantly evolving.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens

Chen

et al. 2020

Vaccines

View full text Add to dashboard Cite

Background: Infectious laryngotracheitis (ILT) is a highly contagious acute respiratory disease of chickens. Antigenic mutation of infectious laryngotracheitis virus (ILTV) may result in a vaccination failure in the poultry industry and thus a protective vaccine against predominant ILTV strains is highly desirable. Methods: The full-length glycoprotein B (gB) gene of ILTV with the two mutated synonymous sites of fowlpox virus (FPV) transcription termination signal sequence was cloned into the insertion vector p12LS, which was co-transfected with wild-type (wt) FPV into chicken embryo fibroblast (CEF) to develop a recombinant fowlpox virus-gB (rFPV-gB) candidate vaccine strain. Furthermore, its biological and immunological characteristics were evaluated. Results: The results indicated that gB gene was expressed correctly in the rFPV by indirect immunofluorescent assay and Western blot, and the rFPV-gB provided a 100% protection in immunized chickens against the challenge of predominant ILTV strains that were screened by pathogenicity assay when compared with the commercialized rFPV vaccine, which only provided 83.3%. Conclusion: rFPV-gB can be used as a potential vaccine against predominant ILTV strains.

show abstract

“…Molecular characterization of ILTV is required to differentiate between field and vaccine strains [15,17,18]. ICP4…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

Yang

Shiro

Fujisawa

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Avian infectious laryngotracheitis (ILT) is a highly contagious viral disease that causes severe economic losses to the poultry industry worldwide. In Southeast Asian countries, including Myanmar, poultry farming is a major industry. Although it is known that infectious respiratory pathogens like infectious laryngotracheitis virus (ILTV) are the major threat to poultry farms, there are no data currently available on the epidemiology of ILTV in Myanmar. In this study, therefore, we conducted molecular detection of ILTV in 20 poultry farms in Myanmar. Results Of the 57 tested oropharyngeal swabs, 10 were positive for ITLV by PCR of a 647 bp region of the thymidine kinase (TK) gene, giving a prevalence of ITLV of 17.5% (10/57). Further sequencing analysis of infected cell protein 4 ( ICP4 ) gene and glycoprotein B, G, and J ( gB , gG , and gJ ) genes indicated that these isolates were field strains. Phylogenetic analysis revealed that the Myanmar strains clustered together in a single branch and were closely related to other reference strains isolated from Asian countries. Conclusions This study demonstrated the presence of ILTV in poultry farms in Myanmar. The genetic characterization analysis performed provides the fundamental data for epidemiological studies monitoring circulating strains of ILTV in Myanmar.

show abstract

MinION sequencing to genotype US strains of infectious laryngotracheitis virus

Cited by 19 publications

References 60 publications

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control – a comprehensive review

Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens

Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

Contact Info

Product

Resources

About