Minisequencing on Functionalised Self-Assembled Monolayer as a Simple Approach for Single Nucleotide Polymorphism Analysis of Cattle

Abstract

We have developed a genetic barcode module, based on a parallel sorting facility of single nucleotide polymorphism for secure individual identification of cattle. Biotinylated allelespecific oligonucleotides were immobilized onto the predefined spots of streptavidin tethered self-assembled monolayers with long chain alkanethiols on biochips. The target DNAs for hybridization and subsequent on-chip minisequencing were produced by multiplex PCR method. After enzymatic extension, only the moiety-modified dideoxynucleotide triphosphate, when coupled to its complementary target sequence, could be detected by the corresponding antibody to the moiety in a specific and sensitive manner. The database SNPZoo was developed for storage of the sequence information consisting of cytosine/thymidine patterns. This SNP chip system can further be used in the detection of any replaceable point mutations occurring in the human and animal genes.

Evolutionary conservation of metabolism explains howDrosophila nutrigenomics can help us understand human nutrigenomics

“…However, at least in Drosophila, the current advantages of using RI lines overcome this potential future inconvenience. Advances in global mapping of single nucleotide polymorphisms (SNPs), such as with "SNP Chips" (Drazinic et al, 2005;Du et al, 2003;Tebbutt et al, 2004;Tonisson et al, 2000), might obviate the need to use RI lines and encourage the further utilization of directly analyzing F 2 lines. A "SNP Chip" can be a microarray-type platform that contains oligos specific for many or all of the SNP differences between two parental strains.…”

“…A "SNP Chip" can be a microarray-type platform that contains oligos specific for many or all of the SNP differences between two parental strains. Non-microarray platforms have also been developed for global-SNP mapping studies (Drazinic et al, 2005;Du et al, 2003;Tebbutt et al, 2004;Tonisson et al, 2000). Rapid and inexpensive DNA genome-size sequencing technologies will also likely increase the practicality of directly genotyping and phenotyping F 2 individuals for QTL mapping studies (Church, 2006).…”