Minocycline hydrochloride nanoliposomes inhibit the production of TNF-&amp;alpha; in LPS-stimulated macrophages

Liu, D; Yang, Peixuan

doi:10.2147/ijn.s34036

Cited by 37 publications

(15 citation statements)

References 32 publications

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“…It is important that the nature of immune suppression in gangliosides is determined because of their potential for reducing xenotransplantation immune rejection. Moreover, the mechanism of cell-mediated xenotransplantation rejection is only partially understood (Mejia-Laguna et al 1971;Ozawa et al 1992;Lin et al 1997;Magnusson et al 2005;Jung et al 2009;Song et al 2010;Liu & Yang 2012). In particular, it is likely that the innate immunity effects that are mediated by gangliosides will influence cell functions and contribute to the strength and complexity of xenotransplantation rejection (Platt et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Effect of gangliosides on LPS stimulation and nitric oxide release in porcine kidney cell line PK15

Lee¹,

Ko²,

Lee³

et al. 2013

Animal Cells and Systems

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(2013) Effect of gangliosides on LPS stimulation and nitric oxide release in porcine kidney cell line PK15, Animal Cells and Systems, 17:5, 341-347, DOI: 10.1080/19768354.2013.839957 To link to this article: https://doi.org/10. 1080/19768354.2013 Gangliosides, which are glycosphingolipids containing sialic acid, play important regulatory roles in cell proliferation and adhesion, survival and immunosuppressive activity. In this study, we investigated whether gangliosides can affect cell viability in the porcine kidney (PK) cell line, PK15, when stimulated with lipopolysaccharide (LPS). As the amount of LPS that PK15 cells were treated with was increased, the cell proliferation decreased, whereas nitric oxide (NO) production increased. High-performance thin-layer chromatography (HPTLC) and immunofluorescence analyses showed that GM3 and GM2 ganglioside expression significantly decreased in LPS-stimulated PK15 compared to unstimulated PK15. UDP-glucose ceramide glucosyltransferase (Ugcg), which catalyzes the initial step in the glycosphingolipid biosynthesis pathway, was knocked-down in PK15 by using short hairpin RNA (shRNA). Western blot and HPTLC analyses showed that the Ugcg protein expression decreased and the ganglioside expression decreased in the Ugcg-knockdown (UKD) PK15. There was a greater decrease in cell proliferation in LPS-stimulated UKD PK15 cells than in LPS-stimulated PK15 cells without the UKD. However, the increase in NO release was greater in LPS-stimulated UKD PK15 cells than in LPS-stimulated PK15 cells without the UKD. These findings suggest that gangliosides may interact with components of the inflammatory response pathway and, thus, are relevant for the design of future therapeutic strategies intended to prolong xenotransplantation.

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Section: Discussionmentioning

confidence: 99%

Effect of gangliosides on LPS stimulation and nitric oxide release in porcine kidney cell line PK15

Lee¹,

Ko²,

Lee³

et al. 2013

Animal Cells and Systems

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“…In another paper, Liu et al developed nanoliposomes as carriers of an anti-inflammatory action drug—minocycline hydrochloride [ 108 ]. The nanoliposomes consist of hydrogenated soy phosphatidylcholine and cholesterol (in a 2:1 molar ratio) and have been prepared via extrusion.…”

Section: Liposomesmentioning

confidence: 99%

Polymeric Carriers for Delivery Systems in the Treatment of Chronic Periodontal Disease

et al. 2020

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Periodontitis (PD) is a chronic inflammatory disease of periodontal tissues caused by pathogenic microorganisms and characterized by disruption of the tooth-supporting structures. Conventional drug administration pathways in periodontal disease treatment have many drawbacks such as poor biodistribution, low selectivity of the therapeutic effect, burst release of the drug, and damage to healthy cells. To overcome this limitation, controlled drug delivery systems have been developed as a potential method to address oral infectious disease ailments. The use of drug delivery devices proves to be an excellent auxiliary method in improving the quality and effectiveness in periodontitis treatment, which includes inaccessible periodontal pockets. This review explores the current state of knowledge regarding the applications of various polymer-based delivery systems such as hydrogels, liposomes, micro-, and nanoparticles in the treatment of chronic periodontal disease. Furthermore, to present a more comprehensive understanding of the difficulties concerning the treatment of PD, a brief description of the mechanism and development of the disease is outlined.

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“…21 "Minocycline" belongs to the tetracycline family, but it has a broader antibacterial spectrum than other tetracyclines and can be applied in the treatment of periodontitis. 21,22 It has been demonstrated that minocycline can affect the immune response caused by cell factors and have a beneficial effect on periodontal health. 23 In this study, minocycline was encapsulated in poly(ethylene glycol)-poly(lactic acid) (PEG-PLA) NPs, which are metabolized to nontoxic CO 2 and H 2 O in the body, 24 and the pharmacokinetics and pharmacodynamics of this formulation were investigated through a periodontitis model in beagle dogs.…”

Section: Introductionmentioning

confidence: 99%

Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs

Yao¹,

Xu²,

Pang

et al. 2014

IJN

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Background Rapid local drug clearance of antimicrobials is a major drawback for the treatment of chronic periodontitis. In the study reported here, minocycline-loaded poly(ethylene glycol)-poly(lactic acid) nanoparticles were prepared and administered locally for long drug retention and enhanced treatment of periodontitis in dogs. Methods Biodegradable poly(ethylene glycol)-poly(lactic acid) was synthesized to prepare nanoparticles using an emulsion/solvent evaporation technique. The particle size and zeta potential of the minocycline-loaded nanoparticles (MIN-NPs) were determined by dynamic light scattering and the morphology of the nanoparticles was observed by transmission electron microscopy. The in vitro release of minocycline from MIN-NPs and in vivo pharmacokinetics of minocycline in gingival crevice fluid, after local administration of MIN-NPs in the periodontal pockets of beagle dogs with periodontitis, were investigated. The anti-periodontitis effects of MIN-NPs on periodontitis-bearing dogs were finally evaluated. Results Transmission electron microscopy examination and dynamic light scattering results revealed that the MIN-NPs had a round shape, with a mean diameter around 100 nm. The in vitro release of minocycline from MIN-NPs showed a remarkably sustained releasing characteristic. After local administration of the MIN-NPs, minocycline concentration in gingival crevice fluid decreased slowly and retained an effective drug concentration for a longer time (12 days) than Periocline ® . Anti-periodontitis effects demonstrated that MIN-NPs could significantly decrease symptoms of periodontitis compared with Periocline and minocycline solution. These findings suggest that MIN-NPs might have great potential in the treatment of periodontitis.

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Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages

Cited by 37 publications

References 32 publications

Effect of gangliosides on LPS stimulation and nitric oxide release in porcine kidney cell line PK15

Effect of gangliosides on LPS stimulation and nitric oxide release in porcine kidney cell line PK15

Polymeric Carriers for Delivery Systems in the Treatment of Chronic Periodontal Disease

Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs

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