Minor dysplastic changes are frequently observed in the bone marrow aspirate in elderly patients without haematological disease

Girodon, François; Favre, Bernardine; Carli, Paule‐Marie; Nash, Nardina; Desbiolles, Norbert; Tatou, Etienne; Maynadié, Marc

doi:10.1046/j.1365-2257.2001.00404.x

Cited by 15 publications

(9 citation statements)

References 5 publications

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“…The identification of lymphoid aggregates in the bone marrow is relatively uncommon (1%-2%), although it has been reported by some to be detected in up to 13% in older individuals [9,14]. Distinguishing between benign and malignant lymphoid aggregates in the bone marrow can be problematic in many cases, and a variety of criteria have been reported to aid in that distinction [2,9,11].…”

Section: Discussionmentioning

confidence: 99%

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

et al. 2013

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Summary Benign lymphoid aggregates are seen in only a minority of bone marrow specimens, but their distinction from non-Hodgkin lymphoma, particularly B-cell lymphomas, can represent a diagnostic challenge. Although criteria have been proposed to help distinguish between benign and malignant aggregates, a detailed description of the distribution patterns of B and T lymphocytes within benign lymphoid aggregates has not been investigated. One hundred thirty-seven cases of bone marrow specimens containing benign aggregates were studied with a panel of immunostains. A subset of these cases was also examined for immunoglobulin gene rearrangements by polymerase chain reaction. The aggregates were categorized based on size, location (paratrabecular or random), presence of infiltrating edges, and distribution of lymphoid cell populations. In addition, we examined 40 cases of bone marrow biopsies with documented malignant lymphoid aggregates for comparison purposes. We report that the distribution of B and T lymphocytes within lymphoid aggregates may serve as a useful criterion to aid in the separation between benign and malignant aggregates. When aggregates exhibit a predominance of T cells, consist of a central core of T cells surrounded by a rim of B cells, or have a mixed distribution of B and T cells, they are more likely to be benign. On the other hand, an increased likelihood of malignancy occurs when aggregates exhibit a predominance of B cells or consist of a central core of B cells surrounded by a rim of T cells (excluding germinal center formation), and assessing other features worrisome of malignancy (large aggregate size, presence of infiltrative edges, cellular atypia, and paratrabecular location, among others) is warranted.

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Section: Discussionmentioning

confidence: 99%

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

et al. 2013

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“…Lymphoid aggregates are common findings in elderly patients. 26,27 In young individuals, they are less common and are often associated with immune or inflammatory disorders, drug therapy or infections. 28,29 In such cases the lymphoid aggregates are usually few in number and small.…”

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confidence: 99%

Bone marrow findings in autoimmune lymphoproliferative syndrome with germline FAS mutation

et al. 2016

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“…Such aggregates can differ in size, are well delimited from surrounding haematopoietic cells, and mostly consist of numerous mature lymphocytes, with a few lymphoid cells, histiocytes, plasma cells and macrophages located among the lymphocytes. The frequency of lymphoid aggregates present in bone marrow aspirates does not usually exceed 20%; however, in autopsy examinations they were seen in 62% of biopsies [6, 7]. The cell composition of such lymphoid cell aggregate can often suggest their character.…”

Section: Discussionmentioning

confidence: 99%

“…A high percentage of more or less polymorphic lymphoid forms, instead of mature lymphocytes, can indicate malignant infiltration. Flow cytometry is often cited as a method that allows verification of the clonal origin of such aggregates [6]. However, a negative result from flow cytometry examination does not exclude the fact that neoplastic lymphoid aggregates might still be present in bone marrow.…”

Section: Discussionmentioning

confidence: 99%

Microscopic examination of bone marrow aspirates in malignant disorders of haematopoiesis—a comparison of two slide preparation techniques

2011

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It is mandatory to perform microscopic examinations of bone marrow aspirates during the diagnosis of many neoplastic haematopoiesis disorders. In 2008, The International Committee for Standardization in Hematology recommended the use of two types of slides for the microscopic evaluation of bone marrow: wedge-spread film and crush film slides. Because these techniques have not yet been compared, we performed such a comparison. Routine bone marrow samples from 250 patients diagnosed due to different neoplastic haematological disorders were evaluated. The major differences between the two compared techniques were identified in 13 patients with non-Hodgkin’s lymphoma, seven patients with systemic mastocytosis and 11 patients with acute leukaemias or myelodysplastic syndromes or chronic myelomonocytic leukaemia. Differences were noted also in many patients with multiple myeloma, but the clinical significance of these discrepancies was rather modest. The major causes of the differences observed seemed to be the dilution of marrow with blood cells and the focal growth of many neoplastic cells. We believe that the crush technique is more advantageous compared to the wedge-spread films. Therefore, we recommend the use of crush films as the primary method for establishing a diagnosis or for making therapeutic decisions based on the microscopic examination of bone marrow.

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Minor dysplastic changes are frequently observed in the bone marrow aspirate in elderly patients without haematological disease

Cited by 15 publications

References 5 publications

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

Benign lymphoid aggregates in the bone marrow: distribution patterns of B and T lymphocytes

Bone marrow findings in autoimmune lymphoproliferative syndrome with germline FAS mutation

Microscopic examination of bone marrow aspirates in malignant disorders of haematopoiesis—a comparison of two slide preparation techniques

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