Minor genetic changes among porcine parvovirus groups are responsible for major distinguishing biological properties

Tijssen, Peter; Bergeron, Jean‐Marie; Dubuc, Roger; Hébert, Benoı̂t

doi:10.1006/smvy.1995.0038

Cited by 14 publications

(6 citation statements)

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“…The PPV1 strains have varying levels of pathogenicity as depicted by the two variants namely porcine parvovirus Kresse (PPV-Kresse) and NADL-2 (PPV-NADL2) (Tijssen et al, 1995). The disparity in their pathogenic potential is believed to be as a result of some amino acid residues substitution in their VP2 structural protein.…”

Section: Viral Pathogenesis and Clinical Manifestationsmentioning

confidence: 99%

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Afolabi

Iweriebor

Okoh

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TDetection of infectious viral agents has been on the increase globally with the advent and usage of more sensitive and selective novel molecular techniques in the epidemiological study of viral diseases of economic importance to the swine industry. The observation is not different for the pig-infecting member of the subfamily Parvovirinae in the family Parvoviridae as the application of novel molecular methods like metagenomics has brought about the detection of many other novel members of the group. Surprisingly, the list keeps increasing day by day with some of them possessing zoonotic potentials. In the last one decade, not less than ten novel swine-infecting viruses have been added to the subfamily, and ceaseless efforts have been in top gear to determine the occurrence and prevalence of the old and new swine parvoviruses in herds of pig-producing countries worldwide. The story, however, is on the contrary on the African continent as there is presently a dearth of information on surveillance initiatives of the viruses among swine herds of pig-producing countries in the region. Timely detection and characterization of the viral pathogens is highly imperative for the implementation of effective control and prevention of its spread. This review therefore presents a concise overview on the epidemiology of novel porcine parvoviruses globally and also provides up-to-date highlights on the reported cases of the viral agents in the African sub-region.

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Section: Viral Pathogenesis and Clinical Manifestationsmentioning

confidence: 99%

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Afolabi

Iweriebor

Okoh

et al. 2019

Infection, Genetics and Evolution

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“…Fetuses infected before day 70 of gestation usually die, whereas fetuses infected at a later time point develop antibodies against PPV, eliminate the virus and survive the infection (Johnson & Collings, 1971). PPV strains can be distinguished by their different pathogenicity (Tijssen et al,1995). Substitution of only a few residues in the VP2 capsid protein is thought to be responsible for distinct biological properties between PPV-NADL-2 and PPV-Kresse in vitro (Bergeron et al,1996;Simpson et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts

Zeeuw

Leinecker

Herwig³

et al. 2007

Journal of General Virology

103

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The pathogenicity of two recent German field isolates of Porcine parvovirus (PPV-27a and PPV-143a) and two vaccine viruses [PPV-NADL-2 and PPV-IDT (MSV)], which are used for the production of inactivated vaccines, was investigated by inoculation of pregnant sows at day 40 of gestation. Post-infection sera of these sows as well as antisera prepared in rabbits by immunization with the four above-mentioned PPV isolates and with the virulent strain PPV-Challenge (Engl.) were tested for their homologous and heterologous neutralization activities. All antisera had high neutralization activity against the vaccine viruses, the PPV-Challenge (Engl.) virus and PPV-143a, but much lower activity against PPV-27a. These results suggest that PPV-27a represents a new antigenic variant or type of PPV and vaccines based on the established vaccine viruses may not be fully protective against this field isolate. PPV-27a has been characterized based on the amino acid sequences of the capsid protein as a member of a new and distinct PPV cluster (Zimmermann et al., 2006). Interestingly, the homologous neutralizing antibody titres of the sera of all three pigs and both rabbits inoculated or immunized with PPV-27a were 100-to 1000-fold lower than the heterologous titres against any of the other viruses. The low homologous neutralizing antibody titres suggest a possible, yet undefined, immune escape mechanism of this PPV isolate.

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“…The genomes of two PPV strains, NADL-2 and Kresse, differ by only 13 nucleotides (nt) and by a 127-nt repeated sequence near the right-end hairpin in the NADL-2 genome, yet they replicate with different efficiencies in both porcine and bovine cells (Fig. 1A) (2,24). Some strains of the parvoviruses minute virus of mice (MVM), canine parvovirus (CPV), and H1 also have variable tandem repeats (65, 60, and 55 nt, respectively) (8).…”

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Genetic Elements in the VP Region of Porcine Parvovirus Are Critical to Replication Efficiency in Cell Culture

Fernandes

Boisvert

Tijssen

2011

J Virol

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Factors controlling porcine parvovirus (PPV) replication efficiency are poorly characterized. Two prototype strains of PPV, NADL-2 and Kresse, differ greatly in pathogenic capacity both in vivo and in vitro, yet their genomic sequence is nearly identical (13 single-nucleotide substitutions and a 127-nucleotide noncoding repeated sequence). We have created a series of chimeras of these strains to identify the genetic elements involved in replication efficiency in the host porcine cell line. While the capsid proteins ultimately determine viral replication fitness, interaction between the NS1 protein and the VP gene occurs and involves interaction with the noncoding repeated sequence.

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Minor genetic changes among porcine parvovirus groups are responsible for major distinguishing biological properties

Cited by 14 publications

References 0 publications

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Increasing diversity of swine parvoviruses and their epidemiology in African pigs

Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts

Genetic Elements in the VP Region of Porcine Parvovirus Are Critical to Replication Efficiency in Cell Culture

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