Minoxidil Nanoparticles Targeting Hair Follicles Enhance Hair Growth in C57BL/6 Mice

Oaku, Yoshihiro; Abe, Akinari; Sasano, Yohei; Sasaki, Fuka; Kubota, Chika; Yamamoto, Naoki; Nagahama, Tohru; Nagai, Noriaki

doi:10.3390/pharmaceutics14050947

Cited by 12 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous previous studies show the possibility of penetration of spherical nanoparticles into hair follicles inducing an enhanced follicular transport of therapeutics [ 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ] and subsequent triggering of release by various external as well as internal trigger mechanisms. Several physical trigger mechanisms, based on diffusion [ 71 ] or infrared light [ 46 ] as well as chemical trigger mechanisms based on pH [ 72 , 73 ] or proteolysis [ 74 , 75 ] were demonstrated in previous studies.…”

Section: Resultsmentioning

confidence: 99%

Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms

et al. 2023

View full text Add to dashboard Cite

Hair follicles constitute important drug delivery targets for skin antisepsis since they contain ≈25% of the skin microbiome. Nanoparticles are known to penetrate deeply into hair follicles. By massaging the skin, the follicular penetration process is enhanced based on a ratchet effect. Subsequently, an intrafollicular drug release can be initiated by various trigger mechanisms. Here, we present novel ultraviolet A (UVA)-responsive nanocapsules (NCs) with a size between 400 and 600 nm containing hydroxyethyl starch (HES) functionalized by an o-nitrobenzyl linker. A phase transfer into phosphate-buffered saline (PBS) and ethanol was carried out, during which an aggregation of the particles was observed by means of dynamic light scattering (DLS). The highest stabilization for the target medium ethanol as well as UVA-dependent release of ethanol from the HES-NCs was achieved by adding 0.1% betaine monohydrate. Furthermore, sufficient cytocompatibility of the HES-NCs was demonstrated. On ex vivo porcine ear skin, a strong UVA-induced release of the model drug sulforhodamine 101 (SR101) could be demonstrated after application of the NCs in cyclohexane using laser scanning microscopy. In a final experiment, a microbial reduction comparable to that of an ethanol control was demonstrated on ex vivo porcine ear skin using a novel UVA-LED lamp for triggering the release of ethanol from HES-NCs. Our study provides first indications that an advanced skin antisepsis based on the eradication of intrafollicular microorganisms could be achieved by the topical application of UVA-responsive NCs.

show abstract

Section: Resultsmentioning

confidence: 99%

Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms

et al. 2023

View full text Add to dashboard Cite

show abstract

“…As target cells, dermal papilla cells have been identified in the in vitro cultural experiments, showing that minoxidil promoted cell proliferation while inhibiting their death by apoptosis [ 2 ]. Animal experiments confirmed also the activation of the hair follicle epithelial stem cells as the leading mechanism of the hair growth promotion by minoxidil [ 3 ]. The adenosine-3-phosphate (ATP)-dependent potassium channels seem to be the major molecular target for minoxidil [ 12 ].…”

Section: Introductionmentioning

confidence: 89%

“…Regarding androgenetic alopecia, which affects both men and women, the treatments are numerous and diverse, including hair transplantation, paramedical physical devices, cosmetics, and pharmacology. Current pharmacological therapies of androgenetic alopecia based on FDA-approved drugs are few, with topical minoxidil (2,4-di-amino-6-piperidinopyrimidine-3-oxide) [ 2 , 3 ] and oral finasteride/dutasteride as active substances [ 4 , 5 , 6 , 7 ]. Combinations of oral and topical therapies have been evaluated as well [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Biomolecules of Fermented Tropical Fruits and Fermenting Microbes as Regulators of Human Hair Loss, Hair Quality, and Scalp Microbiota

Mayer¹,

Weibel²,

Luca³

et al. 2023

Biomolecules

View full text Add to dashboard Cite

Plant-derived secondary metabolites (polyphenols/terpenes/alkaloids) and microbial exometabolites/membrane components of fermented tropical fruits are known as highly bioavailable biomolecules causing skin and hair improvement effects (wound healing, anti-inflammatory, antioxidant, antidiabetic, antiacne, skin/hair microbiota balancing, hair growth-promoting, and hair loss-inhibiting). Caffein is considered as a hair growth promoter. A randomized placebo- and caffein-controlled clinical trial on the efficacy of fermented papaya (FP) plus fermented mangosteen (FM) towards human hair quality and loss was conducted. Shampoo and lotion hair care products containing FP, FM, and caffein as active agents were developed and applied to 154 subjects of both sexes with clinically confirmed androgenic or diffuse alopecia for 3 months. Their clinical efficacy was assessed subjectively by questionnaires filled in by dermatologists/trichologists, and by the objective trichomicroscopical calculations. Hair and scalp skin quality was determined by microbiota pattern and ATP, SH-groups, protein, and malonyl dialdehyde quantification. Comparative clinical data showed that the experimental hair care cosmetics significantly inhibited hair loss, increased hair density/thickness, and improved hair follicle structure versus placebo and caffein controls. The cosmetics with FP and FM substantially normalized the microbiota pattern and increased ATP content in hair follicle, while inhibiting lipid peroxidation in the scalp skin, and SH-group formation in the hair shaft.

show abstract

“…The arrow shows the expression of CD200 that is stained brown. Reprinted or adapted with permission under a Creative Commons Attribution 4.0 International (CC BY 4.0) from ref . Copyright 2022 Pharmaceutics.…”

Section: Development Strategies For Drug Delivery Systems In Alopeciamentioning

confidence: 99%

“…Oaku et al . selected methyl cellulose to obtain nanoparticles containing minoxidil using a bead mill method.…”

Section: Development Strategies For Drug Delivery Systems In Alopeciamentioning

confidence: 99%

Nanodrug Delivery Strategies to Signaling Pathways in Alopecia

Wu,

2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

Over 50% of the global population suffers from hair loss. The mixed results in the treatment of hair loss reveal the limitations of conventional commercial topical drugs. One the one hand, the definite pathogenesis of hair loss is still an enigma. On the other hand, targeted drug carriers ensure the drug therapeutic effect and low side effects. This review highlights the organization and overview of nine crucial signaling pathways associated with hair loss, as well as the development of nanobased topical delivery systems loading the clinical drugs, which will fuel emerging hair loss treatment strategies.

show abstract

Minoxidil Nanoparticles Targeting Hair Follicles Enhance Hair Growth in C57BL/6 Mice

Cited by 12 publications

References 38 publications

Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms

Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms

Biomolecules of Fermented Tropical Fruits and Fermenting Microbes as Regulators of Human Hair Loss, Hair Quality, and Scalp Microbiota

Nanodrug Delivery Strategies to Signaling Pathways in Alopecia

Contact Info

Product

Resources

About