miR-1 association with cell proliferation inhibition and apoptosis in vestibular schwannoma by targeting VEGFA

Abstract

A growing body of research has demonstrated the tumor suppressive function of microRNA (miR)-1 in many cancers. Our study aimed to investigate its role in vestibular schwannoma (VS). We examined miR-1 expression in 95 VS specimens and 79 normal vestibular nerves using quantitative real-time polymerase chain reaction. Moreover, miR-1 mimics, miR-1 inhibitors, and negative control oligonucleotides were transfected into HEI-193 human VS cells to investigate the functional significance of miR-1 expression in this condition at a cellular level. Finally, the role of vascular endothelial growth factor A (VEGFA) in miR-1-mediated HEI-193 cell growth was confirmed. miR-1 levels were significantly reduced in VS specimens compared with normal vestibular nerve tissues (P < 0.001). In addition, low levels of miR-1 were associated with larger tumor volumes. In functional assays, miR-1 suppressed HEI-193 cell proliferation and colony formation, and enhanced apoptosis. VEGFA was verified as a target gene of miR-1, and VEGFA overexpression partially negated the effects of miR-1 on HEI-193 cells. These findings suggest that miR-1 suppresses VS growth by targeting VEGFA, and should be considered as a potential therapeutic target for treatment of this condition.

Lung Endothelial MicroRNA-1 Regulates Tumor Growth and Angiogenesis

“…A previous study on zebrafish reported an antiangiogenic role for miR-1 and miR-206 (62). In line with this report, a few studies indicated that miR-1 targets VEGF in malignant cells (43,44). Our findings in VEGF transgenic mice and in the in vitro VEGF supplementation experiments strongly suggest that the antiangiogenic effects of miR-1 can occur independent of its anti-VEGF properties.…”

“…Previous studies have shown that miR-1 regulates multiple aspects of the tumor cell behavior, such as proliferation (38,39), motility (27,40), apoptosis (41,42), angiogenesis (43,44), and glucose metabolism (45). Two recent studies suggested that miR-1 modulates the paracrine function of cancer-associated fibroblasts (46,47), and one recent report showed that ectopic miR-1 modulates the extracellular vesicles released from glioblastoma tumor cells (48).…”

MicroRNA‐132 suppresses migration and invasion of renal carcinoma cells

“…miRNA is a class of non‐coding single‐stranded RNA molecules . miRNA binds to untranslated region (UTR) of target gene and regulates the expression of target gene at the posttranscriptional levels . It is now found that most protein‐coding genes in mammals are regulated by the expression of miRNAs.…”

Role of microRNAs as novel diagnostic biomarkers and potential therapeutic targets for hearing disorders (Review)

“…The hypothesis that miR-1 may play a suppressive role in this tumor was further confirmed by the transfection of the HEI-193 cell line. Specifically, the overexpression of miR-1 led to a significant reduction in cell proliferation, enhancing the apoptotic process, while the knockout of miR-1 exerted the opposite effect (120). At the same time, in vitro experiments revealed that transfection with miR-1 mimic was negatively related to VEgFA levels.…”

“…These findings suggest that miR-7 could potentially act as a tumor suppressor in vestibular schwannoma formation and growth by inhibiting the EgFR, pak1 and Ack1 signaling pathways (118). Of note, Li et al (120) focused on miR-1, a small non-coding RNA downregulated in various types of cancer (oral cancer, colorectal cancer, lung cancer, etc.) (121)(122)(123).…”