miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer

Ge, Jiyue; Mao, Likai; Xu, Weiqiang; Fang, Wenge; Wang, Ningning; Ye, Dawen; Dong, Zhuang; Guan, Han; Chen, Guan

doi:10.1080/08941939.2020.1738602

Cited by 22 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, certain studies have observed that the function of miR-103a-3p 7 could be characterized as a tumor suppressor gene in several human cancers. In a study investigating the impact of miR-103a-3p on prostate cancer (PCa) cells, enhancement of miR-103a-3p inhibited migration and invasion of PCa cells by regulating the expression of the oncogenic tumor protein D52 (TPD52) [ 41 ]. Moreover, miR-103a-3p inhibited malignant progression of glioma by binding to the FEZF1 3’-UTR [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function

et al. 2022

View full text Add to dashboard Cite

MicroRNAs (miRNAs) have been reported to be involved in the initiation and progression of human tumors including cervical cancer (CC). However, the mechanisms underlying of their actions in CC remain to be fully elucidated. Herein, the differentially expressed miRNAs that were screened based on GSE55940 microarray data retrieved from Gene Expression Omnibus (GEO), and miR-103a-3p was significantly upregulated in CC tissues which was selected as the target miRNA for further research. We also found that high expression of miR-103a-3p was closely associated with histological grades, FIGO stage and distant metastasis as well as reflected poor overall survival. Moreover, miR-103a-3p inhibition decreased the growth capacity of SiHa and HeLa cells by inducing cell apoptosis. And F-box and WD repeat-domain containing protein 7 (FBXW7), a well-known tumor suppressor in many cancer types, was identified as a direct target of miR-103a-3p. It was further found that FBXW7 was significantly downregulated in CC tissues, and it was inversely correlated with miR-103a-3p expression levels. Further investigation demonstrated that FBXW7 upregulation could simulate the roles of miR-103a-3p knockdown in cell viability and apoptosis. Moreover, FBXW7 knockdown efficiently abrogated the influences of CC cells proliferation caused by miR-103a-3p inhibition. Notably, miR-103a-3p could block FBXW7 mediated the downstream transcription factor pathways. Taken together, these findings suggest that miR-103a-3p functions as an oncogene in CC by targeting FBXW7.

show abstract

Section: Discussionmentioning

confidence: 99%

The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function

et al. 2022

View full text Add to dashboard Cite

show abstract

“…SIAH2 mediates the ubiquitination and degradation of NRF1, which affects the stability of mitochondrial-related gene expression in tumor cells, and thus promotes the heterogeneity of tumor metabolism ( 29 ). TPD52 is expressed in a variety of tumor cells, which makes it a target for the treatment of various types of cancer, such as pancreatic cancer, prostate cancer and cervical cancer ( 30 – 32 ). TPD52 is overexpressed in breast cancer and is a significant target gene for regulating breast cancer proliferation and invasion ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a hypoxia‑related prognostic signature for breast cancer

Wang

Xing

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

Hypoxia, an important component of the tumor microenvironment, plays a crucial role in the occurrence and progression of cancer. However, to the best of our knowledge, a systematic analysis of a hypoxia-related prognostic signature for breast cancer is lacking and is urgently required. Therefore, in the present study, RNA-seq data and clinical information were downloaded from The Cancer Genome Atlas (TCGA) and served as a discovery cohort. Cox proportional hazards regression analysis was performed to construct a 14-gene prognostic signature (PFKL, P4HA2, GRHPR, SDC3, PPP1R15A, SIAH2, NDRG1, BTG1, TPD52, MAFF, ISG20, LALBA, ERRFI1 and VHL). The hypoxia-related signature successfully predicted survival outcomes of the discovery cohort (P<0.001 for the TCGA dataset). Three independent Gene Expression Omnibus databases (GSE10886, GSE20685 and GSE96058) were used as validation cohorts to verify the value of the predictive signature (P=0.007 for GSE10886, P=0.021 for GSE20685, P<0.001 for GSE96058). In the present study, a robust predictive signature was developed for patients with breast cancer, and the findings revealed that the 14-gene hypoxia-related signature could serve as a potential prognostic biomarker for breast cancer.

show abstract

“…Moreover, miR-103 is upregulated in colorectal cancer, where it promotes the progression of colorectal cancer by targeting DICER and PTEN [ 18 ]. In recent studies, miR-103a-3p has been reported to promote migration, invasion, and apoptosis of thyroid cancer cells by downregulating LATS1 via Hippo signaling [ 19 ], while in prostate cancer, miR-103a-3p has been shown to suppress the proliferation and invasion by targeting D52 [ 20 ]. However, the biological function of miR-103a-3p in NSCLC has not been fully studied.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-103a-3p Promotes Cell Proliferation and Invasion in Non-Small-Cell Lung Cancer Cells through Akt Pathway by Targeting PTEN

Huhe²,

Lou

2021

BioMed Research International

View full text Add to dashboard Cite

Background. Increasing evidence has suggested that microRNA- (miR-) 103a-3p is crucial for cancer progression. However, the specific mechanism of miR-103a-3p in non-small-cell lung cancer (NSCLC) remains unclear until now. So, it is particularly urgent to clarify the mechanism between them. Methods. qRT-PCR and western blot were used to measure the expression of miR-103a-3p, PTEN, Akt, and p-Akt. Cell biology experiment was applied to detect the biological function of miR-103a-3p in NSCLC cell lines. Moreover, bioinformatics analysis, luciferase reporter assay, and functional complementation analysis were carried out to investigate the target gene. Results. miR-103a-3p was highly expressed in primary NSCLC samples and cell lines. miR-103a-3p mimics promoted the proliferation and invasion of NSCLC cells; miR-103a-3p inhibitor had the opposite effect. A double luciferase reporter gene experiment revealed that miR-103a-3p directly targets the PTEN mRNA 3 ′ UTR region. siPTEN inhibited the proliferation and invasion of NSCLC cells. Further mechanistic studies showed that both overexpression of miR-103a-3p and PTEN knockdown reduced the expression of the p-Akt protein. Overexpression of PTEN partially reversed the cancer-promoting effect of miR-103a-3p. Conclusion. miR-103a-3p promotes the progression of NSCLC via Akt signaling by targeting PTEN, highlighting the role of miR-103a-3p/PTEN/Akt signaling and suggesting miR-103a-3p as a novel therapeutic target for NSCLC.

show abstract

miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer

Cited by 22 publications

References 32 publications

The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function

The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function

Development and validation of a hypoxia‑related prognostic signature for breast cancer

MicroRNA-103a-3p Promotes Cell Proliferation and Invasion in Non-Small-Cell Lung Cancer Cells through Akt Pathway by Targeting PTEN

Contact Info

Product

Resources

About