MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer

Li, Nana; Miao, Yuan; Shan, Yujia; Liu, Bing; Yang, Li; Zhao, Lifen; Li, Jia

doi:10.1038/cddis.2017.119

Cited by 153 publications

(118 citation statements)

References 38 publications

Supporting

Mentioning

113

Contrasting

Order By: Relevance

“…We only identified miR‐145‐5p as being associated with BRCA1, IGF1, PDGFRA, THBS1, CHRM2 , and TNXB . Other miRNAs associated previously with genes in PI3K/Akt‐signaling pathway include miR‐590‐5p, 106b, and miR‐93 with PTEN , miR‐497 with IGF1R, miR‐451 with LKB1, AKT, PI3K , and BCL2 , and miR‐126 with AKT . We observed associations between miR‐497 and miR‐106b‐5p with various genes in the pathway.…”

Section: Discussionsupporting

confidence: 62%

The PI3K/AKT signaling pathway: Associations of miRNAs with dysregulated gene expression in colorectal cancer

Slattery

Mullany

Sakoda

et al. 2017

Molecular Carcinogenesis

View full text Add to dashboard Cite

The PI3K/AKT-signaling pathway is one of the most frequently activated signal-transduction pathways in cancer. We examined how dysregulated gene expression is associated with miRNA expression in this pathway in colorectal cancer (CRC). We used data from 217 CRC cases to evaluate differential pathway gene expression between paired carcinoma and normal mucosa and identify miRNAs that are associated with these genes. Gene expression data from RNA-Seq and miRNA expression data from Agilent Human miRNA Microarray V19.0 were analyzed. We focused on genes most associated with CRC (fold change (FC) of >1.5 or <0.67) that were statistically significant after adjustment for multiple comparisons. Of the 304 genes evaluated, 76 had a FC of <0.67 and 57 had a FC of >1.50; 47 of these genes were associated with miRNA differential expression. There were 145 mRNA:miRNA seed-region matches of which 26 were inversely associated suggesting a greater likelihood of a direct association. Most miRNA:mRNA associations were with factors that stimulated the pathway. For instance, both IL6R and PDGFRA had inverse seed-region matches with seven miRNAs, suggesting that these miRNAs have a direct effect on these genes and may be key elements in activation of the pathway. Other miRNA:mRNA associations with similar impact on the pathway were miR-203a with ITGA4, miR-6071 with ITGAV, and miR-375 with THBS2, all genes involved in extracellular matrix function that activate PI3Ks. Gene expression in the PI3K/Akt-signaling pathway is dysregulated in CRC. MiRNAs were associated with many of these dysregulated genes either directly or in an indirect manner.

show abstract

Section: Discussionsupporting

confidence: 62%

The PI3K/AKT signaling pathway: Associations of miRNAs with dysregulated gene expression in colorectal cancer

Slattery

Mullany

Sakoda

et al. 2017

Molecular Carcinogenesis

View full text Add to dashboard Cite

show abstract

“…Specifically, of the 13 miRNAs included in our signature, there is limited evidence regarding the role of hsa-miR-125-5p in breast cancer progression, while there is some evidence to suggest that miR-301-5p stimulates proliferation and invasion (26) and that hsa-miR-196-5p and hsa-miR-340-5p inhibit progression (27,28). For hsa-miR-30-5p, hsa-miR-130-5p, hsa-miR-93-5p, hsa-miR-503-5p, and hsa-miR-205-5p, there is conflicting evidence regarding their roles in breast cancer progression, with some reports suggesting functions that operate to increase the risk of progression and others suggesting the reverse (29)(30)(31)(32)(33)(34)(35). The apparently conflicting evidence regarding the roles of specific miRNAs in breast cancer prognosis is consistent with the observation that some miRNAs exert both oncogenic and tumor suppressive effects (36,37).…”

Section: Discussionmentioning

confidence: 99%

A miRNA Expression Signature in Breast Tumor Tissue Is Associated with Risk of Distant Metastasis

et al. 2019

View full text Add to dashboard Cite

Dysregulation of miRNA expression may influence breast cancer progression, and experimental evidence suggests that miRNA silencing might suppress breast cancer metastasis. However, the relationship between miRNA and metastasis must be confirmed before this approach can be applied in the clinic. To this end, we conducted a two-stage study in a cohort of 3,760 patients with breast cancer to first identify and then validate the association between miRNA expression and risk of distant metastasis. The first stage (discovery) entailed miRNA sequencing of 126 casecontrol pairs; qPCR was used to validate the findings in a separate set of 80 case-control pairs. The 13 miRNAs most differentially expressed between cases and controls were combined into an miRNA score that was significantly associated with risk of distant metastasis in a logistic regression model that also included clinical variables (tumor size and number of positive lymph nodes) (OR per unit increase in score ¼ 1.30; 95% confidence interval, 1.03-1.66). The results of this study suggest that in women with invasive breast cancer, a miRNA score that incorporates both clinical variables and miRNA expression levels in breast tumor tissue is moderately predictive of risk of subsequent distant metastasis.Significance: A novel predictive scoring system for patients with breast cancer includes clinical variables and the expression levels of 13 miRNAs and may help to identify those at increased risk of distant metastasis. Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): T.E. Rohan, S. Weinmann, O. Loudig Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis):

show abstract

“…Previous studies have demonstrated that KLF4 was regulated by miR-103 in breast cancer 19 ; however, the function of miR-103 and KLF4 in lung cancer still F I G U R E 3 miR-103 is a target of TRHDE-AS1. A, miR-103-binding sequences in the 3′-UTR of TRHDE-AS1 and mutant sites in 3′-UTR of TRHDE-AS1.…”

Section: Klf4 Is a Target Of Mir-103mentioning

confidence: 99%

Overexpression of the long noncoding RNA TRHDE‐AS1 inhibits the progression of lung cancer via the miRNA‐103/KLF4 axis

Zhuan

Chen

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Aim The aim of this study was to explore the role and molecular basis of the long noncoding RNA (lncRNA) TRHDE‐AS1 in lung cancer. Methods We used real‐time polymerase chain reaction to analyze the messenger RNA expression levels of TRHDE‐AS1, miR‐103, and KLF4. The cell viability, proliferation, and invasion rates were assessed via 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, Cell Counting Kit‐8, and Transwell assays to elucidate the role of TRHDE‐AS1. Results Our results demonstrated that the lncRNA TRHDE‐AS1 is mainly located in the cytoplasm and that the cell proliferation and invasion were suppressed in the group of overexpressed TRHDE‐AS1. We also showed that miR‐103 could directly bind to TRHDE‐AS1 and provided evidence of the oncogenic function of miR‐103. Besides, we proved that miR‐103 exerted its function by adjusting the expression level of the tumor‐suppressor gene KLF4, and the expression level was negatively associated with miR‐103. Conclusion In summary, we determined that the effects of TRHDE‐AS1 on proliferation, invasion, and cell death could be rescued by the overexpression of miR‐103. Our experiments demonstrate that the TRHDE‐AS1/miR‐103/KLF4 axis may provide new evidence for understanding the molecular basis of lung cancer.

show abstract

MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer

Cited by 153 publications

References 38 publications

The PI3K/AKT signaling pathway: Associations of miRNAs with dysregulated gene expression in colorectal cancer

The PI3K/AKT signaling pathway: Associations of miRNAs with dysregulated gene expression in colorectal cancer

A miRNA Expression Signature in Breast Tumor Tissue Is Associated with Risk of Distant Metastasis

Overexpression of the long noncoding RNA TRHDE‐AS1 inhibits the progression of lung cancer via the miRNA‐103/KLF4 axis

Contact Info

Product

Resources

About