miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Zhou, Jiamin; Sun, Xianglin; Zhang, Xinyu; Yang, Huan; Jiang, Zheng‐Lin; Luo, Qianqian; Liu, Yifei; Wang, Guohua

doi:10.1186/s12885-022-09603-3

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…Notably, the levels in EV Q and EV UC were significantly higher than in the EV M condition, with EV M exhibiting the lowest recovery rate. We next assessed a specific set of six miRNAs known to be present in saliva [44] and for which dysregulations are associated to diverse pathologies [53–58]. For all the targets, we clearly observed that miRNAs were enriched within EVs, confirming several previous observations [44, 45].…”

Section: Discussionsupporting

confidence: 77%

Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

Boulestreau,

Molina,

Ouedraogo

et al. 2024

Preprint

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Extracellular vesicles (EVs), crucial mediators in cell-to-cell communication, are implicated in both homeostatic and pathological processes. Their detectability in easily accessible peripheral fluids like saliva positions them as promising candidates for non-invasive biomarker discovery. However, the lack of standardized methods for salivary EVs isolation greatly limits our ability to study them. Therefore, we rigourously compared salivary EVs isolated using two scalable techniques - co-precipitation and immuno-affinity - against the long-established but labor-intensive ultracentrifugation method. Employing Cryo-Electron Microscopy, Nanoparticle Tracking Analysis, Western blots (WB), and proteomics, we identified significant method-dependent variances in the size, concentration, and protein content of EVs. Importantly, our study uniquely demonstrates the ability of EV isolation to detect specific biomarkers that remain undetected in whole saliva by WB. RT-qPCR analysis targeting six miRNAs confirmed a consistent enrichment of these miRNAs in EV-derived cargo across all three isolation methods. We also found that pre-filtering saliva samples with 0.22 or 0.45 um pores adversely affects subsequent analyses. Our findings highlight the untapped potential of salivary EVs in diagnostics and advocate for the co-precipitation method as an efficient, cost-effective, and clinically relevant approach for small-volume saliva samples. This work not only sheds light on a neglected source of EVs but also paves the way for their application in routine clinical diagnostics.

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Section: Discussionsupporting

confidence: 77%

Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

Boulestreau,

Molina,

Ouedraogo

et al. 2024

Preprint

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“…A total of 360 significantly different lncRNAs were identified in BC and adjacent normal tissues across the two databases (Figure 1E; Figure S1A; Table S5). The human BC cell lines MDA‐MB‐231 and MCF‐7 exhibited differences in aggressiveness and malignancy, with MDA‐MB‐231 being highly metastatic and malignant 29,32 . This finding was also corroborated by a wound healing assay (Figure 1F,G).…”

Section: Resultssupporting

confidence: 63%

A novel long non‐coding RNALINC00524 facilitates invasion and metastasis through interaction with TDP43 in breast cancer

Sun,

Li,

et al. 2024

J Cellular Molecular Medi

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Breast cancer (BC) remains a significant health concern worldwide, with metastasis being a primary contributor to patient mortality. While advances in understanding the disease's progression continue, the underlying mechanisms, particularly the roles of long non‐coding RNAs (lncRNAs), are not fully deciphered. In this study, we examined the influence of the lncRNA LINC00524 on BC invasion and metastasis. Through meticulous analyses of TCGA and GEO data sets, we observed a conspicuous elevation of LINC00524 expression in BC tissues. This increased expression correlated strongly with a poorer prognosis for BC patients. A detailed Gene Ontology analysis suggested that LINC00524 likely exerts its effects through RNA‐binding proteins (RBPs) mechanisms. Experimentally, LINC00524 was demonstrated to amplify BC cell migration, invasion and proliferation in vitro. Additionally, in vivo tests showed its potent role in promoting BC cell growth and metastasis. A pivotal discovery was LINC00524's interaction with TDP43, which leads to the stabilization of TDP43 protein expression, an element associated with unfavourable BC outcomes. In essence, our comprehensive study illuminates how LINC00524 accelerates BC invasion and metastasis by binding to TDP43, presenting potential avenues for therapeutic interventions.

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“…Both miR-107 and PTEN are important players in carcinogenesis. miR-107 has a potential role in regulating NEDD9 in the invasion, migration and proliferation of breast cancer [ 49 ]. Additionally, miR-107 is hypothesized to deactivate the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in a hypopharyngeal squamous cell carcinoma (HSCC) study [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

In Prostate Cancer Cells Cytokines Are Early Responders to Gravitational Changes Occurring in Parabolic Flights

Schulz

Dietrichs

Wehland

et al. 2022

IJMS

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The high mortality in men with metastatic prostate cancer (PC) establishes the need for diagnostic optimization by new biomarkers. Mindful of the effect of real microgravity on metabolic pathways of carcinogenesis, we attended a parabolic flight (PF) mission to perform an experiment with the PC cell line PC-3, and submitted the resulting RNA to next generation sequencing (NGS) and quantitative real-time PCR (qPCR). After the first parabola, alterations of the F-actin cytoskeleton-like stress fibers and pseudopodia are visible. Moreover, numerous significant transcriptional changes are evident. We were able to identify a network of relevant PC cytokines and chemokines showing differential expression due to gravitational changes, particularly during the early flight phases. Together with differentially expressed regulatory lncRNAs and micro RNAs, we present a portfolio of 298 potential biomarkers. Via qPCR we identified IL6 and PIK3CB to be sensitive to vibration effects and hypergravity, respectively. Per NGS we detected five upregulated cytokines (CCL2, CXCL1, IL6, CXCL2, CCL20), one zink finger protein (TNFAIP3) and one glycoprotein (ICAM1) related to c-REL signaling and thus relevant for carcinogenesis as well as inflammatory aspects. We found regulated miR-221 and the co-localized lncRNA MIR222HG induced by PF maneuvers. miR-221 is related to the PC-3 growth rate and MIR222HG is a known risk factor for glioma susceptibility. These findings in real microgravity may further improve our understanding of PC and contribute to the development of new diagnostic tools.

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miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancer

Cited by 10 publications

References 41 publications

Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

A novel long non‐coding RNALINC00524 facilitates invasion and metastasis through interaction with TDP43 in breast cancer

In Prostate Cancer Cells Cytokines Are Early Responders to Gravitational Changes Occurring in Parabolic Flights

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