miR-1207-5p and miR-1266 suppress gastric cancer growth and invasion by targeting telomerase reverse transcriptase

Chen, L; Mh, Lü; Zhang, D; Nb, Hao; Fan, Y-H.; Wu, Yan-Guang; Sm, Wang; Xie, Ruiqin; Dc, Fang; Zhang, H; Cj, Hu; Sm, Yang

doi:10.1038/cddis.2013.553

Cited by 120 publications

(78 citation statements)

References 41 publications

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“…Although altered expression of several miRNAs has been involved in the regulation of hTERT [12–15], the epigenetic mechanisms that caused elevated hTERT expression in OC remain poorly characterized. In this study, we have attempted to identify tumor suppressor miRNAs that modulate hTERT expression in OC cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

Bai

Wang

et al. 2017

PLoS ONE

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Human telomerase reverse transcriptase (hTERT) plays a crucial role in ovarian cancer (OC) progression. However, the mechanisms underlying hTERT upregulation in OC, and the specific microRNAs (miRNAs) involved in the regulation of hTERT in OC cells, remains unclear. We performed a bioinformatics search to identify potential miRNAs that bind to the 3'-untranslated region (3'-UTR) region of the hTERT mRNA. We examined the expression levels of miR-532/miR-3064 in OC tissues and normal ovarian tissues, and analyzed the correlation between miRNA expression and OC patient outcomes. The impacts of miR-532/miR-3064 on hTERT expression were evaluated by western blot analysis and hTERT 3'-UTR reporter assays. We investigated the effects of miR-532/miR-3064 on proliferation and invasion in OC cells. We found that miR-532 and miR-3064 are down-regulated in OC specimens. We observed a significant association between reduced miR-532/miR-3064 expression and poorer survival of patients with OC. We confirmed that in OC cells, these two miRNAs downregulate hTERT levels by directly targeting its 3'-UTR region, and inhibited proliferation, EMT and invasion of OC cells. In addition, the overexpression of the hTERT cDNA lacking the 3'-UTR partially restored miR-532/miR-3064-inhibited OC cell proliferation and invasion. The silencing of hTERT by siRNA oligonucleotides abolished these malignant features, and phenocopied the effects of miR-532/miR-3064 overexpression. Furthermore, overexpression of miR-532/miR-3064 inhibits the growth of OC cells in vivo. Our findings demonstrate a miR-532/miR-3064-mediated mechanism responsible for hTERT upregulation in OC cells, and reveal a possibility of targeting miR-532/miR-3064 for future treatment of OC.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

Bai

Wang

et al. 2017

PLoS ONE

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“…The overexpression of hTERT was induced with a HIV-based lentiviral vector system, as previously described 11. The packaged lentiviruses were referred as lenti-hTERT and lenti-Control.…”

Section: Methodsmentioning

confidence: 99%

hTERT promotes the invasion of gastric cancer cells by enhancing FOXO3a ubiquitination and subsequent ITGB1 upregulation

et al. 2015

Gut

116

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“…Chen L et al showed that miR- 1207 suppresses gastric cancer growth and invasion by targeting telomerase reverse transcriptase (hTRET) [2]. MiR-1207-5p was significantly upregulated in gastric cancer patients without lymph node metastasis (LNM) compared with those with LNM [3].…”

Section: Introductionmentioning

confidence: 99%

miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1

et al. 2016

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We previously reported that miR-1207-5p can inhibit epithelial-mesenchymal transition (EMT) induced by growth factors such as EGF and TGF-β, but the exact mechanism is unclear. Here we identified that Colony stimulating factor 1 (CSF1) is a target gene of miR-1207-5p. CSF1 controls the production, differentiation and function of macrophage and promotes the release of proinflammatory chemokines. We showed that miR-1207-5p inhibited lung cancer cell A549 proliferation, migration and invasion in vitro, and suppressed the STAT3 and AKT signalings. miR-1207-5p overexpression can increase HUVEC angiogenesis, and can modulate the M2 phenotype of macrophage. miR-1207-5p also significantly inhibited A549 cells metastasis in a nude mouse xenograft model. miR-1207-5p and CSF1 expression levels and their relationship with lung cancer survival and metastasis status were assayed by means of a lung cancer tissue microarray. Macrophage is an essential part of the tumor microenvironment, thus the miR-1207-5p-CSF1 axis maybe a new regulator of lung cancer development through modulating the tumor microenvironment.

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miR-1207-5p and miR-1266 suppress gastric cancer growth and invasion by targeting telomerase reverse transcriptase

Cited by 120 publications

References 41 publications

MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

hTERT promotes the invasion of gastric cancer cells by enhancing FOXO3a ubiquitination and subsequent ITGB1 upregulation

miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1

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