MiR‐122 inhibits epithelial mesenchymal transition by regulating P4HA1 in ovarian cancer cells

Duan, Yanan; Dong, Yinghui; Dang, Rongguang; Zhi-Hui, HU; Yang, Ye; Hu, Yueyun; Cheng, Jianxin

doi:10.1002/cbin.11052

Cited by 47 publications

(32 citation statements)

References 30 publications

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“…miR-122 inhibited epithelial mesenchymal transition by regulating P4HA1 expression in OC cells [43]. In addition, miR-122 regulated LHCGR expression, which was crucial for mediating LH action in growing follicles via modulating LRBP levels during FSH-induced follicle growth [96]. miR-122 has been shown to increase LHR mRNA levels by modulating the expression of LRBP through the regulation of SREBP activation, which was crucial for regulating key reproductive processes, such as ovulation and CL function [97].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Zou

Zhao

et al. 2020

BMC Genomics

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Background: Fertility is an important economic trait in the production of meat goat, and follicular development plays an important role in fertility. Although many mRNAs and microRNAs (miRNAs) have been found to play critical roles in ovarian biological processes, the interaction between mRNAs and miRNAs in follicular development is not yet completely understood. In addition, less attention has been given to the study of single follicle (dominant or atretic follicle) in goats. This study aimed to identify mRNAs, miRNAs, and signaling pathways as well as their interaction networks in the ovarian follicles (large follicles and small follicles) of uniparous and multiple Chuanzhong black goats at estrus phase using RNAsequencing (RNA-seq) technique. Results: The results showed that there was a significant difference in the number of large follicles between uniparous and multiple goats (P < 0.05), but no difference in the number of small follicles was observed (P > 0.05). For the small follicles of uniparous and multiple goats at estrus phase, 289 differentially expressed mRNAs (DEmRNAs) and 16 DEmiRNAs were identified; and for the large follicles, 195 DEmRNAs and 7 DEmiRNAs were identified. The functional enrichment analysis showed that DE genes in small follicles were significantly enriched in ovarian steroidogenesis and steroid hormone biosynthesis, while in large follicles were significantly enriched in ABC transporters and steroid hormone biosynthesis. The results of quantitative real-time polymerase chain reaction were consistent with those of RNA-seq. Analysis of the mRNA-miRNA interaction network suggested that CD36 (miR-122, miR-200a, miR-141), TNFAIP6 (miR-141, miR-200a, miR-182), CYP11A1 (miR-122), SERPINA5 (miR-1, miR-206, miR-133a-3p, miR-133b), and PTGFR (miR-182, miR-122) might be related to fertility, but requires further research on follicular somatic cells. Conclusions: This study was used for the first time to reveal the DEmRNAs and DEmiRNAs as well as their interaction in the follicles of uniparous and multiple goats at estrus phase using RNA-seq technology. Our findings provide new clues to uncover the molecular mechanisms and signaling networks of goat reproduction that could be potentially used to increase ovulation rate and kidding rate in goat.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Zou

Zhao

et al. 2020

BMC Genomics

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“…Additionally, high P4HA1 expression is a poor prognostic factor for head and neck squamous cell carcinoma (51), oral squamous cell carcinoma (52), and prostate cancer (53). In ovarian cancer P4HA1 promotes migration, invasion, EMT and metastasis formation (54). In glioma, P4HA1 promotes the transdifferentiation of glioma stem cells into endothelial cells leading to the formation of vascular basement membranes (55) and has been considered as a prognostic marker for high-grade glioma (56).…”

Section: Collagen-prolyl Hydroxylation In Cancer Progressionmentioning

confidence: 99%

Proline Metabolism in Tumor Growth and Metastatic Progression

et al. 2020

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Cancer cells show a formidable capacity to survive under stringent conditions, to elude mechanisms of control, such as apoptosis, and to resist therapy. Cancer cells reprogram their metabolism to support uncontrolled proliferation and metastatic progression. Phenotypic and functional heterogeneity are hallmarks of cancer cells, which endow them with aggressiveness, metastatic capacity, and resistance to therapy. This heterogeneity is regulated by a variety of intrinsic and extrinsic stimuli including those from the tumor microenvironment. Increasing evidence points to a key role for the metabolism of non-essential amino acids in this complex scenario. Here we discuss the impact of proline metabolism in cancer development and progression, with particular emphasis on the enzymes involved in proline synthesis and catabolism, which are linked to pathways of energy, redox, and anaplerosis. In particular, we emphasize how proline availability influences collagen synthesis and maturation and the acquisition of cancer cell plasticity and heterogeneity. Specifically, we propose a model whereby proline availability generates a cycle based on collagen synthesis and degradation, which, in turn, influences the epigenetic landscape and tumor heterogeneity. Therapeutic strategies targeting this metabolic-epigenetic axis hold great promise for the treatment of metastatic cancers.

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“…miR-122 has been reported as a tumor-inhibiting factor in variety of human cancers ( 29 ). An increasing amount of evidence suggests that miR-122-5p is significantly downregulated, and inhibits cell proliferation and metastasis in numerous types of cancer, including nasopharyngeal carcinoma ( 30 ), hepatocellular carcinoma ( 31 ) and bile duct carcinoma ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA ZEB2‑AS1 affects cell proliferation and apoptosis via the�miR‑122‑5p/PLK1�axis in acute myeloid leukemia

Guan

Liu

Wang³

et al. 2020

Int J Mol Med

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Acute myeloid leukemia (AML) is a highly heterogeneous disease featured by the clonal accumulation of immature myeloid cells. Zinc finger E-box binding homeobox 2 (ZEB2)-antisense RNA 1 (AS1) has been verified to participate in the progression of several types of cancer, including AML. However, the potential mechanisms of ZEB2-AS1 in AML have not yet been fully elucidated. The present study aimed to elucidate the role and regulatory mechanisms of ZEB2-AS1 in AML. The expression of ZEB2-AS1, microRNA-122-5p (miRNA/miR-122-5p) and polo-like kinase 1 (PLK1) was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in AML tissues or cells. Cell proliferation and apoptosis were examined by methyl thiazolyl tetrazolium (MTT) assay and apoptosis assay, respectively. The protein levels were examined by western blot analysis. The targeted sequence between miR-122-5p and ZEB2-AS1 or PLK1 was predicted using an online database and verified by dual-luciferase reporter assay. A mouse tumor xenograft model was established to confirm the effects of ZEB2-AS1 on tumor growth in vivo. The results revealed that the expression levels of ZEB2-AS1 and PLK1 were upregulated, while those of miR-122-5p were downregulated in AML tissues and cells. The knockdown of ZEB2-AS1 inhibited proliferation and induced apoptosis in vitro , and inhibited tumor growth in vivo . By experimental verification, ZEB2-AS1 was found to negatively regulate miR-122-5p expression and PLK1 was found to be a target gene of miR-122-5p. Furthermore, ZEB2-AS1 was verified to regulate the expression of PLK1 by sponging miR-122-5p in AML cells. On the whole, the findings of the present study demonstrate that ZEB2-AS1 promotes cell proliferation and inhibits apoptosis, at least partly by targeting PLK1 mediated by miR-122-5p in AML cells.

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MiR‐122 inhibits epithelial mesenchymal transition by regulating P4HA1 in ovarian cancer cells

Cited by 47 publications

References 30 publications

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Proline Metabolism in Tumor Growth and Metastatic Progression

Long non‑coding RNA ZEB2‑AS1 affects cell proliferation and apoptosis via the�miR‑122‑5p/PLK1�axis in acute myeloid leukemia

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