miR-1246-overexpressing exosomes suppress UVB-induced photoaging via regulation of TGF-β/Smad and attenuation of MAPK/AP-1 pathway

Yuan, Limin; Zhang, Yue; Huang, Fang-Zhou; Gao, Fei; Li, Jian; Xing, Feng; Wang, Hui; Wang, Yu-Shuai

doi:10.1007/s43630-022-00304-1

Cited by 19 publications

(19 citation statements)

References 41 publications

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“…Five Sankyo Denki sunlamps (G15T8E lamps, 312 nm) were used to conduct UVB irradiation on Kunming mice. The procedure was carried out according to our previous report (27). The intensity of UVB was progressively increased during the study: 100 mJ/cm 2 daily in the first week, and increased to 200 mJ/cm 2 every other day from 2nd to 8th week.…”

Section: Methodsmentioning

confidence: 99%

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Zhang

Yuan

Huang

et al. 2023

Photochem & Photobiology

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UVB‐induced photoaging is characterized by wrinkle formation, slackness and senile plaques, affecting the health and beauty of human being. Our previous study revealed that exosomes derived from adipose‐derived stem cells (ADSCs) could efficiently alleviate UVB‐induced photodamage. However, the functional ingredients in exosomes were undefined. LncRNA H19, one of the well‐researched lncRNAs in exosomes, exhibits multiple physiological effects. This study aims to demonstrate the photo‐protective role of lncRNA H19 on skin photoaging in UVB‐irradiated human skin fibroblasts cells (HSFs) and Kunming mice. LncRNA H19‐overexpressing exosomes (H19‐Exo) were isolated from the supernatant of ADSCs infected with lncRNA H19‐loaded lentivirus. The results showed that H19‐Exo significantly inhibited MMPs production, DNA damage and ROS generation while enhancing procollagen type I synthesis in UVB‐irradiated HSFs. Meanwhile, H19‐Exo markedly reversed epidermal thickening and collagen degradation in UVB‐irradiated mice. Furthermore, luciferase reporter assays indicated that lncRNA H19 acted as a sponge for miR‐138 expression, and SIRT1 was targeted by miR‐138. Evidence from both in vitro and in vivo studies also revealed that H19‐Exo could enhance SIRT1 expression by knocking down miR‐138. In conclusion, lncRNA H19 served as a therapeutic candidate in treating UVB‐induced skin photoaging by upregulation of SIRT1 via miR‐138.

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Section: Methodsmentioning

confidence: 99%

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Zhang

Yuan

Huang

et al. 2023

Photochem & Photobiology

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“…In the beginning for 4 weeks, mice were exposed to UVB radiation without samples treatment. From the 5th week, the mice were daubed with SP, HP, and Vc three times a week following UVB irradiation according to previous method 31 . The normal group was not exposed to UVB radiation.…”

Section: Methodsmentioning

confidence: 99%

“…From the 5th week, the mice were daubed with SP, HP, and Vc three times a week following UVB irradiation according to previous method. 31 The normal group was not exposed to UVB radiation.…”

Section: All Experimental Protocols Were Agreed Upon With the Animal ...mentioning

confidence: 99%

Protective effect of Salvia plebeia R. Br ethanol extract on UVB‐induced skin photoaging in vitro and in vivo

Guo

Zhang

Wang

et al. 2023

Photoderm Photoimm Photomed

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BackgroundUV exposure is one of the primary factors responsible for photoaging, causing the increase in matrix metalloproteinases (MMPs) and the reduction in collagen. Salvia plebeia R. Br (SP), as an herbaceous plant, contains abundant flavonoids and possesses excellent anti‐inflammatory and antioxidant activities. This study aimed to investigate the photoprotective effects of SP on UVB‐induced photodamage in immortalized human keratinocytes (HaCaTs) and Kunming mice, as well as its main active components such as homoplantaginin (HP).MethodsCCK‐8 was applied to detect the cell viability in UVB‐irradiated or non‐irradiated HaCaTs. Commercial kits were used to evaluate the levels of ROS, MDA, SA‐β‐Gal, MMP‐1, and IL‐6. The expression of MAPK and TGF‐β/Smad pathways was detected by western blot. HE and Masson's trichrome staining were performed to examine the epidermis thickness and collagen degradation of Kunming mice.ResultsOur results found that SP and HP notably decreased UVB‐induced ROS, MDA, and SA‐β‐Gal production, and inhibited MMP‐1 and IL‐6 secretion by inhibiting the MAPK signaling pathway. In addition, SP and HP significantly promoted type I procollagen synthesis by activation of TGF‐β/Smad pathway. Consistently, the in vivo experiments also indicated that SP and HP had a photoprotective effect, which significantly reversed UVB‐induced epidermis thickness and collagen degradation.ConclusionThis study demonstrated that SP effectively could protect skin from UVB‐induced photoaging, while HP acted as the active substance in SP. All these findings provided a new strategy for skin photoaging treatment.

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“…Adipose‐derived stem cell exosomes were found to effectively inhibit excessive ROS and SA‐β‐Gal generation induced by senescent HDFs during in vitro expansion, suggesting their potential for rejuvenating these cells 89 . ADSC MiR‐1246‐overexpressing exosomes markedly increased procollagen type 1 secretion by activating the TGF‐β/Smad pathway 90 . Bovine milk exosomes induce type 1 collagen synthesis through the STAT6 pathway in human fibroblast 91 and donkey and human milk exosomes activate the p‐ERK pathway, promoting cell cycle and proliferation of skin fibroblasts in vitro 92 .…”

Section: Mechanism Of Stem Cell Exosome Activity Against Skin Photoagingmentioning

confidence: 99%

“…Based on this, MMP activities may represent the primary mechanism that regulates the photoaging of the skin 102,103 . Research found that the reduction of ADSC miR‐1246‐overexpressing exosomes on MMP‐1 secretion appears to rely on the inhibition of the MAPK/AP‐1 signaling pathway 90 . Human bone marrow MSC (BM‐MSC)‐ExomiR‐29b3p inhibited the degradation of collagen type 1 and the production of MMPs in photoaging, and they were also eliminated by the reduced miR29b3p (MMP‐2 was the target gene of miR29b3p) 104 …”

Section: Mechanism Of Stem Cell Exosome Activity Against Skin Photoagingmentioning

confidence: 99%

Mesenchymal stem cell‐derived exosomes and skin photoaging: From basic research to practical application

Wang,

Shen,

Song

et al. 2023

Photoderm Photoimm Photomed

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BackgroundSkin photoaging is a condition caused by long‐term exposure to ultraviolet irradiation, resulting in a variety of changes in the skin, such as capillary dilation, increased or absent pigmentation, dryness, sagging, and wrinkles. Stem cells possess a remarkable antioxidant capacity and the ability to proliferate, differentiate, and migrate, and their main mode of action is through paracrine secretion, with exosomes being the primary form of secretion. Stem cell‐derived exosomes contain a variety of growth factors and cytokines and may have great potential to promote skin repair and delay skin ageing.MethodsThis review focuses on the mechanisms of UV‐induced skin photoaging, the research progress of stem cell exosomes against skin photoaging, emerging application approaches and limitations in the application of exosome therapy.ResultExosomes derived from various stem cells have the potential to prevent skin photoaging.ConclusionThe combination with novel materials may be a key step for their practical application, which could be an important direction for future basic research and practical applications.

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miR-1246-overexpressing exosomes suppress UVB-induced photoaging via regulation of TGF-β/Smad and attenuation of MAPK/AP-1 pathway

Cited by 19 publications

References 41 publications

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Protective effect of Salvia plebeia R. Br ethanol extract on UVB‐induced skin photoaging in vitro and in vivo

Mesenchymal stem cell‐derived exosomes and skin photoaging: From basic research to practical application

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