miR‐125a‐3p aggravates ox‐LDL‐induced HUVEC injury through BAMBI

Abstract

Atherosclerosis (AS) is a chronic inflammatory disease characterized by the formation of atherosclerotic plaque in the intima of arteries. Among the known regulators of atherosclerosis, microRNAs (miRNAs) have been reported to play critical roles in lipoprotein homeostasis and plaque formation. But the roles of microRNA‐125a‐3p (miR‐125a‐3p) in the pathogenesis of AS remain unknown. Human umbilical vein endothelial cells (HUVECs) were treated with oxidized low‐density lipoprotein (ox‐LDL) to construct the vascular injury model of AS pathogenesis in vitro. miR‐125a‐3p and BMP and activin membrane‐bound inhibitor (BAMBI) expression levels in HUVECs were then measured by quantitative real‐time polymerase chain reaction and western blot. The viability and apoptosis of HUVECs were analyzed by Cell Counting Kit‐8 assay, TUNEL assay, and flow cytometry, respectively. The relationship between BAMBI 3′‐untranslated region and miR‐125a‐3p was validated by dual luciferase reporter gene assay. miR‐125a‐3p expression was raised in HUVECs induced with ox‐LDL. In HUVECs, miR‐125a‐3p enhanced the effects of ox‐LDL treatment on repressing the viability and promoting the apoptosis of cells. Additionally, BAMBI was confirmed as a direct target of miR‐125a‐3p and BAMBI overexpression reversed the effects of miR‐125a‐3p on HUVECs. miR‐125a‐3p aggravates the dysfunction of HUVECs induced by ox‐LDL via BAMBI, which implies that miR‐125a‐3p is involved in the pathogenesis of AS.

Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

“…This network was established to investigate the mechanism of YTHDC1. Results showed that miR-125a-3p involved in regulating cell proliferation and apoptosis [ [51] , [52] , [53] ]. Our Result showed that miR-125-3p may contribute to PAECs dysfunction and pulmonary remodeling.…”

Inhibition of lncRNA NFIA-AS1 Alleviates Abnormal Proliferation and Inflammation of Vascular Smooth Muscle Cells in Atherosclerosis by Regulating miR-125a-3p/AKT1 Axis

“…MiR-125a-3p may be closely related to AS pathogenesis. Xia and Zeng found that miR-125a-3p aggravates Ox-LDL-induced HUVEC injury through BAMBI [ 31 ]. Hu et al confirmed that miR-125a-3p can effectively inhibit the function of VSMCs and the occurrence of vascular stenosis by targeting MAPK1 [ 32 ].…”

lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis

“…Through LncRNA2Target prediction and dual-luciferase reporter assay, we identified lncRNA-Gm5532 as a ceRNA by targeting miR-125a-3p. miR-125a-3p was reported to be involved in the progression of multiple diseases, including atherosclerosis [22] , cataracts [23] , rheumatoid arthritis [24] , papillary thyroid carcinoma [25] , breast cancer [26] , and colorectal cancer [27] . In addition, miR-125a-3p is a potential regulator of bone homeostasis.…”