MiR-125b-5p and miR-181b-5p inhibit keratinocyte proliferation in skin by targeting Akt3

Zheng, Yunpeng; Cai, Bingjie; Li, Xuyang; Li, Dongqin; Yin, Guangwen

doi:10.1016/j.ejphar.2019.172659

Cited by 27 publications

(25 citation statements)

References 30 publications

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“…1B). The level of Ki-67 protein in local keratinocytes from patients with psoriasis can be reduced by the overexpression of miR-125b-5p or miR-181b-5p [12]. We found that the expression of Ki-67 increased as the expression of miR-30a-5p and miR-514a-3p decreased in the local tissue lesions of CA patients (Fig.…”

Section: Discussionmentioning

confidence: 59%

Dysregulation of autophagy-associated microRNAs in condyloma acuminatum

Shi¹,

Lu²,

Zheng³

et al. 2020

Preprint

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Objectives This study was designed to investigate the miRNAs that regulate the cell proliferation of condyloma acuminatum (CA) lesions and their targets.Methods The expression of Ki-67 in 26 CA patients compared with 10 healthy controls was assessed by immunohistochemistry. And the different miRNAs in 4 CA patients and 4 control cases were analyzed by bioinformatics. PCR was used to validate the expression of screened miRNA and its corresponding target genes.Results The expression of Ki-67 was abnormally increased in CA compared with healthy controls ( P <0.05). The comparison of the control group with the CA group revealed 81 differentially expressed miRNAs, of which 56 were downregulated and 25 were upregulated. Two of the differentially expressed miRNAs, miR-30a-5p and miR-514a-3p, are associated with cell proliferation and their target genes are autophagy-related protein (Atg) 5 and Atg12, and Atg3 and Atg12, respectively. PCR results showed that the expression levels of miR-30a-5p and miR-514a-3p were decreased in CA patients compared with healthy controls ( P <0.05), whereas the expression of Atg5, Atg12 and Atg3 was increased ( P <0.05). The expression of the autophagy proteins microtubule-associated protein 1 light chain 3 (LC3) and P62/SQSTM1 (P62) was abnormally increased in the local lesion tissue of the 26 patients with CA compared with the 10 healthy controls, as assessed by immunohistochemistry ( P <0.05).Conclusions Our results suggest that autophagy levels may be modulated by has-miRNA30a-5p and has-miRNA514a-3p in CA patients, leading to dysregulated cell proliferation.

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Section: Discussionmentioning

confidence: 59%

Dysregulation of autophagy-associated microRNAs in condyloma acuminatum

Shi¹,

Lu²,

Zheng³

et al. 2020

Preprint

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“… Human epidermal keratinocytes (HEKs) Akt3 Akt/mTOR Signaling Upregulation of miR-181b-5p inhibited HEKs proliferation at least partly by targeting Akt3. [ 33 ] miR‐181b 28 patients diagnosed with psoriasis and 20 healthy controls who underwent plastic surgery were recruited. Human epidermal keratinocytes (HEKs) TLR4 TLR4 pathway miR‐181b negatively regulates the proliferation of HEKs in psoriasis and might provide new insights for seeking novel targets of treatment and prognosis of psoriasis.…”

Section: Down-regulated Mirnas In Psoriasismentioning

confidence: 99%

The eminent roles of ncRNAs in the pathogenesis of psoriasis

Ghafouri‐Fard

Eghtedarian

Taheri

et al. 2020

Non-coding RNA Research

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Psoriasis is a chronic immune-related disorder in which both genetic and environmental parameters are involved. Recent studies have demonstrated dysregulation of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in the peripheral blood or skin lesions of patients with psoriasis. While a number of lncRNAs such as MEG3, AL162231.4 and NONHSAT044111 have been down-regulated in the course of psoriasis, others including PRINS, MIR31HG, RP6‐65G23.1, MSX2P1, SLC6A14-1:1, NR_003062 have been up-regulated. Moreover, expressions of several miRNAs have been dysregulated in this disorder. Among dysregulated miRNAs are miR-126, miR-143, miR-19a and miR-155 whose diagnostic roles in the psoriasis have also been assessed. Dysregulated non-coding RNAs in this disorder participate in the regulation of chemokine signaling pathway and immune response, control of epidermal development and skin barrier as well as modulation of function of certain subsets of T cells. Besides, these transcripts possibly regulate activity of NF-κΒ, mTOR, MAPK and JAK-STAT signaling pathways. Besides, expression levels of circRNAs have been decreased in the psoriasis lesions. Massive alterations in the levels of lncRNAs and miRNAs in the psoriasis lesions or peripheral blood of affected individuals show participation of these transcripts in the pathogenesis of this disorder.

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“…The DE-miRNAs are involved in a third mechanism represented by the regulation of repair pathways after inflammation. For example, miR-320a is involved in intestinal mucosal reconstitution and repair after inflammation (60), and miR-125b inhibits proliferation and promotes differentiation of keratinocytes in the skin (61). Therefore, the capability of cerumen to down-regulate miR-320a and miR-125b may result in keratinocyte proliferation, which in turn may accelerate wound healing and homeostasis restitution.…”

Section: Discussionmentioning

confidence: 99%

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa

et al. 2020

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Otitis externa is one of the most common diseases in dogs. It is associated with bacteria and yeast, which are regarded as secondary causes. Cerumen is a biological substance playing an important role in the protection of ear skin. The involvement of cerumen in immune defense is poorly understood. MicroRNAs can modulate the host immune response and can provide promising biomarkers for several inflammatory and infectious disorder diagnosis. The aims of this study were to profile the cerumen miRNA signature associated with otitis externa in dogs, integrate miRNAs to their target genes related to immune functions, and investigate their potential use as biomarkers. Cerumen was collected from healthy and otitis affected dogs and the expression of miRNAs was profiled by Next Generation Sequencing; the validation of the altered miRNAs was performed using RT-qPCR. The potential ability of miRNAs to modulate immune-related genes was investigated using bioinformatics tools. The results pointed out that 32 miRNAs, of which 14 were up-and 18 down-regulated, were differentially expressed in healthy vs. otitis-affected dogs. These results were verified by RT-qPCR. To assess the diagnostic value of miRNAs, ROC analysis was carried out, highlighting that 4 miRNAs are potential biomarkers to discriminate otitis-affected dogs. Bioinformatics showed that cerumen miRNAs may be involved in the modulation of host immune response. In conclusion, we have demonstrated for the first time that miRNAs can be efficiently extracted and quantified from cerumen, that their profile changes between healthy and otitis affected dogs, and that they may serve as potential biomarkers. Further studies are necessary to confirm their diagnostic value and to investigate their interaction with immune-related genes.

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MiR-125b-5p and miR-181b-5p inhibit keratinocyte proliferation in skin by targeting Akt3

Cited by 27 publications

References 30 publications

Dysregulation of autophagy-associated microRNAs in condyloma acuminatum

Dysregulation of autophagy-associated microRNAs in condyloma acuminatum

The eminent roles of ncRNAs in the pathogenesis of psoriasis

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa

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