MiR-125b promotes migration and invasion by targeting vitamin D receptor in renal cell carcinoma

Abstract

Background To investigate the expression of miR-125b and vitamin D receptor (VDR) in renal cell carcinoma (RCC) and assess the possible association between them. Then, to elucidate whether miR-125b can regulate the expression of VDR and affect proliferation and metastasis in RCC.Methods The expression of miR-125b was detected by quantitative real-time polymerase chain reaction (RT-PCR) in RCC cell lines. MiR-125b mimic and inhibitor were employed to measure the function and behavior of miR-125b in RCC cell lines. The relationship between miR-125 and VDR was verified using luciferase assays, and their expression was also examined in primary tumor and normal peritumoral kidney tissues in 20 clear cell RCC (ccRCC) samples.

miR‑5590‑3p inhibits the proliferation and metastasis of renal cancer cells by targeting ROCK2 to inhibit proliferation, migration and invasion

“…Çaykara et al (27) indicated that abnormally downregulated miR-124 was associated with the tumor stage, tumor size and neutrophil count in patients with RCC, and restoration of miR-124 expression may be effective in the treatment of RCC. He et al (28) reported that miR-125b was significantly upregulated in RCC tissues, which was negatively correlated with vitamin D receptor (VDR) levels. Through mediating VDR, miR-125 is capable of enhancing proliferative and migratory rates of RCC cells, suggesting miR-125 may be a potential therapeutic target of RCC.…”