miR‑126/VCAM‑1 regulation by naringin suppresses cell growth of human non‑small cell lung cancer

Abstract

Abstract. Certain studies have indicated that naringin possesses various pharmacological activities including anti-aging, anti-oxidation, anticancer, cardiovascular and cerebrovascular disease prevention, in addition to anti-hepatic effects. The present study explores the anticancer effect of naringin on human small cell lung cancer H69AR cells. Cell growth and apoptosis rates of H69AR cells were measured by MTT or flow cytometry, which demonstrated naringin suppressed cell growth and induced apoptosis of H69AR cells. MicroRNA (miR)-126 expression and levels of phosphorylated protein kinase B (AKT), mechanistic target of rapamycin (mTOR), nuclear factor (NF)-κB and vascular cell adhesion molecule 1 (VCAM-1) proteins were detected by quantitative polymerase chain reaction and western blotting. It was identified that naringin increased miR-126 expression and suppressed the phosphorylation of AKT, mTOR, NF-κB and VCAM-1 proteins in H69AR cells. Suppression of miR-126 expression reduced the anticancer effects of naringin on H69AR cells, reversed the naringin-induced reduction of phosphoinositide 3-kinase/AKT/mTOR, and suppressed VCAM-1 protein levels. However, close of miR-126 expression did not affect the levels of NF-κB protein in H69AR cells. In summary, naringin exhibits its anti-cancer effect by suppressing cell growth of small cell lung cancer cells through miR-126/VCAM-1 signaling pathway.

Naringin Protects Against Interleukin 1β (IL-1β)-Induced Human Nucleus Pulposus Cells Degeneration via Downregulation Nuclear Factor kappa B (NF-κB) Pathway and p53 Expression

“…Naringin is a flavonoid extracted from natural plant and has special pharmacological properties such as antiarthritic, anticancer, anti-oxidant and anti-inflammatory activities [24][25][26][27]. Moreover, previous studies have reported that naringin could exert anti-inflammatory and anti-oxidative effects through downregulating NF-kB signaling pathway [21].…”

Mapping Pharmacological Network of Multi-Targeting Litchi Ingredients in Cancer Therapeutics

“…lung, cervical, gastric, and breast cancer Raha et al, 2015;Yoshinaga et al, 2016;Chen et al, 2018a) promoting apoptosis BAX, CASP1/3/9, FADD, FAS, MTCO2, NFKB, TP53, CDKL2…”

Naringin suppressing the PI3K/Akt signaling pathway as a drug target in BIU87 cells

“…Recently, flavonoids isolated from herbs have attracted many researchers interest because of their anticancer effects on various cancer cells. Naringin, which is mainly recognized as blocking PI3K/Akt pathway, appears through cell cycle arrest and apoptosis and finally interrupts cells growth [22][23][24] . It was reported that naringin could inhibit breast cancer, lung cancer and cervical cancer, and so on 8 [25][26][27] .…”

“…Naringin, which is mainly recognized as blocking PI3K/Akt pathway, appears through cell cycle arrest and apoptosis and finally interrupts cells growth [22][23][24] . It was reported that naringin could inhibit breast cancer, lung cancer and cervical cancer, and so on 8 [25][26][27] . Consistently, we confirmed the antitumor potential of naringin on bladder cancer and focused on how naringin induced PI3K/Akt pathway in bladder cancer cells.…”