miR-129-3p, as a diagnostic and prognostic biomarker for renal cell carcinoma, attenuates cell migration and invasion via downregulating multiple metastasis-related genes

Chen, Xuanyu; Ruan, Anming; Wang, Xuegang; Wang, Han; Wang, Rong; Lou, Ning; Ruan, Hailong; Qiu, Bin; Yang, Hongmei; Zhang, Xiaoping

doi:10.1007/s00432-014-1690-7

Cited by 63 publications

(57 citation statements)

References 41 publications

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“…MiR-129-2 had a higher methylation ratio, indicating its potential as a methylation biomarker in early diagnosis of ESCC [7]. Considering that miR-129-3p was markedly decreased in ccRCC, it may act as a promising diagnostic biomarker for distinguishing ccRCC from benign tumors and normal tissues and an independent prognostic biomarker in ccRCC [29]. As shown in a recent study, the detection of gastric juice miR-129-1-3p and miR-129-2-3p may be an innovative choice for the diagnosis of gastric cancer [28].…”

Section: Mir-129 As a Biomarker For Cancer Diagnosis And Prognosismentioning

confidence: 99%

“…Ectopic expression of miR-129-3p robustly impaired RCC cell invasive and migratory properties. MiR-129-3p exerted its anti-metastatic function through modulating multiple metastasis-related genes, including SOX4, phosphorylation of focal adhesion kinase and MMP-2/9 expression [29]. MMP9 was also proved a target gene of miR-129 to govern metastasis in NSCLC cells [79].…”

Section: Mir-129 and Metastasismentioning

confidence: 99%

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MicroRNA-129 in Human Cancers: from Tumorigenesis to Clinical Treatment

Gao¹,

Feng²,

Han³

et al. 2016

Cell Physiol Biochem

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Emerging evidence has shown that microRNAs (miRNAs) play essential roles in regulating human cancers development and progression. However, the underlying mechanisms remain to be further explored. MiRNAs are a class of endogenous, non-coding, 18-24 nucleotide length single-strand RNAs that moderate gene expression primarily at post-transcriptional level. There is a growing body of literature that recognizes the importance of microRNA (miR)-129 during the development of cancers. Aberrant expression of miR-129 has been detected in various types of human cancers and the validated target genes are involved in cancer-related biological processes such as DNA methylation, cell proliferation, apoptosis, cell cycle, and metastasis. In this review, we summarized the roles of miR-129 family members and their target genes in tumorigenesis and clinical treatment of human cancers, highlighting the potential roles of miR-129 as biomarkers for cancer diagnosis and prognosis, and promising tools for cancer treatment.

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Section: Mir-129 As a Biomarker For Cancer Diagnosis And Prognosismentioning

confidence: 99%

Section: Mir-129 and Metastasismentioning

confidence: 99%

MicroRNA-129 in Human Cancers: from Tumorigenesis to Clinical Treatment

Gao¹,

Feng²,

Han³

et al. 2016

Cell Physiol Biochem

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“…Growing evidence showed that miRNAs could act as novel biomarkers or therapy agent for various cancers, inculding RCC (17). For instance, the expression of was miR-129-3p significantly decreased in RCC, as a molecular diagnostic marker to distinguish RCC from benign tumors and normal tissues and a potential prognostic marker for RCC (18). Chen et al showed that miR-141 serves as a potential biomarker for discriminating RCC from normal tissues and a crucial suppressor of RCC cell proliferation and metastasis by modulating the EphA2/p-FAK/p-AKT/MMPs signaling cascade (19).…”

Section: Discussionmentioning

confidence: 99%

miR-137 inhibits renal cell carcinoma growth in vitro and in vivo

Zhang

2016

Oncology Letters

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Abstract. MicroRNA (miR)-137 has been reported to be underexpressed and involved in various cell processes and to have antitumor effects in a range of tumors, but so far not in renal cell carcinoma (RCC). The aim of the present study was to investigate the clinical significance and role of miR-137 in RCC. The expression levels of miR-137 were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in 50 cases of paired RCC tissues and adjacent normal tissues, and in RCC cell lines. The role of miR-137 in the growth and survival of RCC cells was assessed with several in vitro approaches and in nude mice models. The results of the RT-qPCR showed that the miR-137 expression was downregulated in the RCC tissues and cell lines. The in vitro assay showed that ectopic expression of miR-137 robustly impaired RCC cell proliferation, migratory and invasive properties, and increased the induction of cell apoptosis properties. The in vivo assay demonstrated that enforced miR-137 suppressed tumor growth in xenograft nude mice models. In addition, miR-137 was indicated to inhibit the activation of phosphoinositide 3 kinase/protein kinase B signal pathway, which contributes to the inhibition of RCC growth. These findings indicate that miR-137 functions as tumor suppressor in RCC, suggesting that miR-137 may be a potential therapeutic target for RCC. IntroductionRenal cell carcinoma (RCC) accounts for 3% of all adult malignancies and is the third most common urological cancer worldwide, with the highest mortality rate of RCC at >40% (1). Approximately 65,150 novel cases and 13,680 mortalities were estimated for 2013 in the USA (1). Clear cell RCC (ccRCC) is the largest subtype of RCC and accounts for 70% of RCCs (2). Despite the increasingly early detection of RCC and more frequent use of surgery, the mortality rate has not changed significantly since 1990 and ~30% patients with localized RCC develop post-operative metastatic recurrence (3). The current adjuvant therapy for metastatic RCC remains extremely limited, as RCC is relatively unaffected by chemotherapy or radiotherapy (4). Therefore, a better understanding of the mechanisms involved in the pathogenesis of RCC and more effective therapeutic approaches are urgently required.MicroRNAs (miRNAs) are a small class of non-coding RNAs of 19-25 nucleotides in length. miRNAs are known to be important in the pathogenesis of cancer, from initiation to metastasis, primarily through interaction with the 3' untranslated region (3'UTR) of various target genes, which results in target gene translational silence or cleavage (5). miRNAs are known to be involved in multiple tumorigenic steps in human cancers, including in cell growth, apoptosis, migration and invasion (6-9). Of these miRNAs, miR-137 is an notable member that is located on chromosome 1p22 and has been indicated to be underexpressed in non-small lung (10), gastric (11), colorectal (12) and breast cancer (13). The restoration of miR-137 expression has been shown to inhibit cancer cell growth, mi...

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“…Su et al revealed that let-7d may suppress RCC growth, metastasis and tumor macrophage infiltration by targeting COL3A1 and chemokine ligand-7 (22). A study by Chen et al demonstrated that miRNA-129-3p attenuates cell migration and invasion of RCC by downregulating multiple metastasis-associated genes, and may also act as a diagnostic and prognostic biomarker for RCC (23). Wu et al revealed that miRNA-133b was downregulated in RCC cell lines and inhibited cell proliferation, migration and invasion of RCC cells (24).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-27a functions as a tumor suppressor in renal cell carcinoma by targeting epidermal growth factor receptor

Sun

et al. 2016

Oncology Letters

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Abstract. Numerous studies have suggested that microRNAs (miRNAs) are vital in the development of various types of human cancers, including renal cell carcinoma (RCC), and the regulation of tumor progression and invasion. However, the effect of miRNA-27a (miR-27a) on the tumorigenesis of RCC is unclear. The aim of the present study was to investigate the function of miR-27a and identify its possible target genes in RCC cells. In the present study, cell proliferation, migration and invasion and the percentage of apoptotic cells were detected by methylthiazol tetrazolium assays, Annexin V analysis, wound-healing assays and Transwell invasion assays. Western blot analysis was performed to validate the protein expression level and assess whether the epidermal growth factor receptor (EGFR) was a target gene of miR-27a. A tumor xenograft animal model was used to detect the role of miR-27a on RCC cell growth in vivo. The present study demonstrated that miR-27a significantly suppressed human RCC 786-O cell proliferation and induced cell apoptosis. Restoration of miR-27 also resulted in 786-O cell migration and invasion inhibition. Furthermore, upregulated miR-27a attenuated RCC tumor growth in the tumor xenograft animal model. The present results suggested that miR-27a functions as a tumor suppressor in RCC. The western blot analysis assay revealed that EGFR was a novel target of miR-27a. The growth suppression of RCC cells was attributed partly to the downregulation of the cell cycle by ERFR inhibition. The present findings may aid in the understanding of the molecular mechanism of miR-27a in the tumorigenesis of RCC, and may provide novel diagnostic and therapeutic options for RCC.

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miR-129-3p, as a diagnostic and prognostic biomarker for renal cell carcinoma, attenuates cell migration and invasion via downregulating multiple metastasis-related genes

Abstract: miR-129-3p may act as a promising diagnostic biomarker for discriminating ccRCC from benign tumors and normal tissues and an independent prognostic biomarker in ccRCC. miR-129-3p may exert its anti-metastatic function through modulating multiple targets.

Cited by 63 publications

References 41 publications

MicroRNA-129 in Human Cancers: from Tumorigenesis to Clinical Treatment

MicroRNA-129 in Human Cancers: from Tumorigenesis to Clinical Treatment

miR-137 inhibits renal cell carcinoma growth in vitro and in vivo

MicroRNA-27a functions as a tumor suppressor in renal cell carcinoma by targeting epidermal growth factor receptor

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