miR-130a-3p regulates steroid hormone synthesis in goat ovarian granulosa cells by targeting the PMEPA1 gene

Zhu, Lu; Jing, Jing; Qin, Shuaiqi; Zheng, Qi; Lu, Jiani; Zhu, Cuiyun; Liu, Ya; Fang, Fugui; Li, Yunsheng; Ling, Yinghui

doi:10.1016/j.theriogenology.2021.02.012

Cited by 17 publications

(9 citation statements)

References 27 publications

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“…29 miR-130a-3p targets the prostate transmembrane protein androgen induced 1 (PMEPA1) gene to inhibit estradiol and progesterone synthesis in goat granulosa cells. 30 However, there are relatively few studies on miRNA modulation of steroidogenesis in chicken granulosa cells, and our results will further enrich the understanding of miRNA regulation. miR-181b-5p is a potential diagnostic biomarker for several types of cancers.…”

Section: Discussionmentioning

confidence: 68%

“…miR-99a-5p suppressed the expression of the target gene frizzled class receptor 5 ( FZD5 ) and thus blocked steroid hormone secretion in goat granulosa cells . miR-130a-3p targets the prostate transmembrane protein androgen induced 1 ( PMEPA1 ) gene to inhibit estradiol and progesterone synthesis in goat granulosa cells . However, there are relatively few studies on miRNA modulation of steroidogenesis in chicken granulosa cells, and our results will further enrich the understanding of miRNA regulation.…”

Section: Discussionmentioning

confidence: 99%

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Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lin,

Gong,

Sun

et al. 2023

J. Agric. Food Chem.

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Steroid hormones secreted by granulosa cells are essential for maintaining normal development of chicken follicles. Our previous sequencing data indicated that miR-181b-5p and RAS-related protein 1B (RAP1B) appeared to function in chicken granulosa cells, which was further explored in this study. The results suggested that miR-181b-5p facilitated the aggregation of lipid droplets and the synthesis of progesterone. In contrast, RAP1B astricted lipid deposition and progesterone secretion. Cotransfection of the RAP1B overexpression vector with miR-181b-5p mimic eliminated the promoting effect of miR-181b-5p. Dual-luciferase reporter assay confirmed that miR-181b-5p bound directly to the 3′ untranslated region (3′ UTR) of RAP1B. We also found that miR-181b-5p and RAP1B reduced and enhanced the phosphorylation levels of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), respectively. The application of ERK1/2 activators and inhibitors demonstrated that ERK1/2 is a negative regulator of lipid deposition and progesterone synthesis. In conclusion, we revealed that miR-181b-5p accelerated lipid deposition and progesterone synthesis through the RAP1B/ERK1/2 pathway in chicken granulosa cells. miR-181b-5p and RAP1B may serve as new biomarkers in breeding to improve chicken reproductive performance and prevent ovary-related diseases.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lin,

Gong,

Sun

et al. 2023

J. Agric. Food Chem.

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“…MiRNAs are now known to be small noncoding RNAs that affect the expression of mRNA at the posttranscriptional level. Studies have shown that miRNAs play an essential role in the regulation and development of reproduction [47][48][49]. For example, the upregulation of miR-181a-5p promotes cell proliferation and cell cycle progression and inhibits apoptosis to resist MG-HS infection [50], and miR-145 protects GCs against oxidative stress-induced apoptosis by targeting KLF4 [51].…”

Section: Discussionmentioning

confidence: 99%

SEMA4G targeted by miR‐363‐5p regulates the proliferation of granulosa cells in Yunshang black goats

Chen

Wang

et al. 2023

Animal Research and One Health

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Proliferation of granular cells (GCs) plays an important role in ovary development, providing energy and a microenvironment for oocyte ovulation. In this study, we explored the spatiotemporal expression of SEMA4G and its effects on the growth and development of goat GCs using primary GCs cultured in vitro as a model. The results showed that the expression level of SEMA4G was significantly higher in the ovaries of high‐fertility goats than in those of low‐fertility goats (p < 0.05). The mRNA and protein expression levels of the cell proliferation markers of GCs were significantly increased after the overexpression of SEMA4G in goat primary GCs. The EdU and CCK8 results showed that cell viability was elevated in goat GCs and that proliferation was promoted by an increase in the number of proliferating cells. The proliferation of goat GCs was significantly inhibited by SEMA4G inhibition (p < 0.05). The results of online miRNA and target gene prediction software and dual luciferase activity analysis confirmed that SEMA4G could bind to mi‐363‐5p and was one of its target genes. The RT‒qPCR results showed that the expression level of miR‐363‐5p was significantly lower in the ovaries of high‐fertility goats than in those of low‐fertility goats in contrast to the expression level of SEMA4G (p < 0.05). After the overexpression of miR‐363‐5p in goat GCs, the expression of SEMA4G was significantly suppressed (p < 0.05). Collectively, the results of this study could lay the foundation for exploring the molecular mechanisms by which SEMA4G and miR‐363‐5p regulate the growth and development of goat GCs and provide targets for breeding high‐fertility goats.

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“…MicroRNAs (miRNAs) play an important role in the regulation of E 2 and Pg synthesis in ovarian GCs. MiR-132 has been found to target and suppress the mRNA expression of Star which in turn suppresses steroid hormone production [ 14 ]; miR-31, which targets HSD17B14 and FSHR, affects apoptosis and steroid hormone metabolism in porcine ovarian GCs [ 15 ]; and miR-130a-3p regulates steroid hormone synthesis in goat ovarian GCs by targeting the PMEPA1 gene [ 16 ]. In addition, DNA methylation modifications are also important for the expression of genes involved in E 2 and Pg synthesis.…”

Section: Introductionmentioning

confidence: 99%

Paternal genetic effects of cadmium exposure during pregnancy on hormone synthesis disorders in ovarian granulosa cells of offspring

et al. 2023

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The aim of this study was to investigate the paternal genetic intergenerational and transgenerational genetic effects of cadmium (Cd) exposure during pregnancy on estradiol (E2) and progesterone (Pg) synthesis in the ovarian granulosa cells (GCs) of offspring. Pregnant SD rats were intragastrically exposed to CdCl2 (0, 0.5, 2.0, 8.0 mg/kg) from days 1 to 20 to produce the F1 generation, F1 males were mated with newly purchased females to produce the F2 generation, and the F3 generation was obtained in the same way. Using this model, Cd-induced hormone synthesis disorders in GCs of F1 have been observed [8]. In this study, altered serum E2 and Pg levels in both F2 and F3 generations showed a nonmonotonic dose‒response relationship. In addition, hormone synthesis-related genes (Star, Cyp11a1, Cyp17a1, Cyp19a1, Sf-1) and miRNAs were observed to be altered in both F2 and F3. No differential changes in DNA methylation modifications of hormone synthesis-related genes were observed, and only the Adcy7 was hypomethylated. In summary, paternal genetic intergenerational and transgenerational effects exist in ovarian GCs E2 and Pg synthesis disorders induced by Cd during pregnancy. In F2, the upregulation of StAR and CYP11A1, and changes in the miR-27a-3p, miR-27b-3p, and miR-146 families may be important, while changes in the miR-10b-5p and miR-146 families in F3 may be important.

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miR-130a-3p regulates steroid hormone synthesis in goat ovarian granulosa cells by targeting the PMEPA1 gene

Cited by 17 publications

References 27 publications

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

Lipid Deposition and Progesterone Synthesis Are Increased by miR-181b-5p through RAP1B/ERK1/2 Pathway in Chicken Granulosa Cells

SEMA4G targeted by miR‐363‐5p regulates the proliferation of granulosa cells in Yunshang black goats

Paternal genetic effects of cadmium exposure during pregnancy on hormone synthesis disorders in ovarian granulosa cells of offspring

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