MiR-130a regulates neurite outgrowth and dendritic spine density by targeting MeCP2

Zhang, Yunjia; Chen, Mengmeng; Qiu, Zilong; Hu, Keping; McGee, W Ambrose; Chen, Xiaoping; Liu, Jianghong; Zhu, Li; Wu, Jane Y.

doi:10.1007/s13238-016-0272-7

Cited by 32 publications

(21 citation statements)

References 73 publications

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“…In this study, we present evidence that cytoskeletal disruption also contributes to neurodegeneration in the ENS during early stages of PD; miR-130a-3p expression was upregulated in psA30P mice. This miRNA inhibits neurite outgrowth and reduces dendritic spine density 74 . It also targets MECP2, which induces symptoms in patients with Rett syndrome that are similar to those seen in PD and dopamine metabolism disorders 75 .…”

Section: Discussionmentioning

confidence: 99%

Functional and molecular early enteric biomarkers for Parkinson’s disease in mice and men

Gries

Christmann

Schulte

et al. 2020

Preprint

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Parkinson's disease (PD) usually has a late clinical onset. The lack of early biomarkers for the disease represents a major challenge for developing timely treatment interventions. Here, we use an -synuclein-overexpressing transgenic (Th-1-SNCA-A30P) mouse model of PD to identify appropriate candidate markers in the gut for early stages of PD before hallmark symptoms begin to manifest. A30P mice did not show alterations in gait parameters at 2 months of age, and these mice were therefore defined as pre-symptomatic A30P mice (psA30P). We discovered early functional motility changes in the gut and early molecular dysregulations in the myenteric plexus of psA30P mice by comparative protein and miRNA profiling and cell culture experiments. We found that the proteins neurofilament light chain, vesicle-associated membrane protein 2 and calbindin 2, together with the miRNAs that regulate them, are potential biomarkers of early PD that may facilitate timely treatment and/or prevention of PD in men.

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Section: Discussionmentioning

confidence: 99%

Functional and molecular early enteric biomarkers for Parkinson’s disease in mice and men

Gries

Christmann

Schulte

et al. 2020

Preprint

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“…miR-130a is an anti-angiogenic transcription factor. The study of Jiang et al (15) showed that the expression of miR-130a is positively proportional to the cerebral water content in rats with cerebral edema, suggesting that miR-130a may be associated with the formation of cerebral edema (16). Furthermore, Altintas et al (17) found that miR-130a also plays an important regulatory role in diabetic rats with transient CI.…”

Section: Discussionmentioning

confidence: 99%

miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway

Wang

et al. 2020

Exp Ther Med

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Effect of micro ribonucleic acid (miR)-130a on neuronal apoptosis in rats with cerebral infarction (CI) was studied to explore whether phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (Akt) is involved in the regulation of neuronal apoptosis. Thirty-six Sprague-Dawley (SD) rats were randomly divided into blank control group, model group and miR-130a low-expression group. miR-130a was determined by quantitative polymerase chain reaction (qPCR), the content of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10 was detected using the enzyme-linked immunosorbent assay (ELISA) kits, and the neuronal apoptosis level in each group was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. The neurobehavioral score was significantly lower in model group than that in blank control group (P<0.01), while it was significantly higher in miR-130a low-expression group than that in model group (P<0.01). Compared with blank control group, the model group had obviously increased content of TNF-α and IL-6 (P<0.01), decreased content of IL-10 (P<0.01), more apoptotic neurons (P<0.01), higher expression of caspase-3 (P<0.01), and obviously lower Bcl-2/Bax (P<0.01). Moreover, expression of phosphorylated (p)-PTEN, PI3K and p-Akt in brain tissues was remarkably lower in the model group than those in the blank control group (P<0.01). The expression level of miR-130a in brain tissues of CI rats is significantly increased. miR-130a promotes the release of inflammatory factors and facilitates neuronal apoptosis through suppressing the PTEN/PI3K/Akt signaling pathway.

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“…These GO categories and KEGG pathways are closely related to the proliferation and differentiation of NSCs. Studies have shown that miR-106b could promote the renewal of NSCs and inhibit their differentiation 29 ; miR-130a and miR-138 could inhibit axon growth and regeneration 30 , 31 ; miR-20b overexpression could downregulate the expressions of Map2 and Tubb3 (well-known neuronal markers) 32 . In this study, we observed that these miRNAs were upregulated in the early stage of NSCs after miR-31 overexpression.…”

Section: Discussionmentioning

confidence: 99%

mRNA and miRNA expression profile reveals the role of miR-31 overexpression in neural stem cell

Gao

Xiao

et al. 2020

Sci Rep

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A detailed understanding of the character and differentiation mechanism of neural stem cells (NSCs) will help us to effectively utilize their transplantation to treat spinal cord injury. In previous studies, we found that compared with motor neurons (MNs), miR-31 was significantly high-expressed in NSCs and might play an important role in the proliferation of NSCs and the differentiation into MNs. To better understand the role of miR-31, we characterized the mRNA and miRNAs expression profiles in the early stage of spinal cord-derived NSCs after miR-31 overexpression. There were 35 mRNAs and 190 miRNAs differentially expressed between the miR-31 overexpression group and the control group. Compared with the control group, both the up-regulated mRNAs and miRNAs were associated with the stemness maintenance of NSCs and inhibited their differentiation, especially to MNs, whereas the down-regulated had the opposite effect. Further analysis of the inhibition of miR-31 in NSCs showed that interfering with miR-31 could increase the expression of MNs-related genes and produce MNs-like cells. All these indicated that miR-31 is a stemness maintenance gene of NSCs and has a negative regulatory role in the differentiation of NSCs into MNs. This study deepens our understanding of the role of miR-31 in NSCs, provides an effective candidate target for effectively inducing the differentiation of NSCs into MNs, and lays a foundation for the effective application of NSCs in clinic.

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MiR-130a regulates neurite outgrowth and dendritic spine density by targeting MeCP2

Cited by 32 publications

References 73 publications

Functional and molecular early enteric biomarkers for Parkinson’s disease in mice and men

Functional and molecular early enteric biomarkers for Parkinson’s disease in mice and men

miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway

mRNA and miRNA expression profile reveals the role of miR-31 overexpression in neural stem cell

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