MiR‐130b increases fibrosis of HMC cells by regulating the TGF‐β1 pathway in diabetic nephropathy

Ma, Yufei; Shi, Jingxia; Wang, Feifei; Li, Shipeng; Wang, Jie; Zhu, Chengfeng; Li, Liping; Lu, Haibo; Li, Chun; Yan, Jize; Zhang, Xin; Jiang, Hong

doi:10.1002/jcb.27688

Cited by 19 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hsa-miR-130b-3p is a well-known marker, studied in cellular and animal models, involved at different level in the progression and maintenance of different kinds of cancer, 75 , 76 but it has also been shown to be involved in chondrogenesis and osteogenesis, 77 associated with obesity, insulin resistance, and other pathophysiological processes. 45 , 78 A previous study has shown how inflammatory signals, such as TNFα and IL-1β, induce its action in adipose inflamed tissue. 44 So far, the presented results show a higher expression of hsa-miR-130b-3p in patients with low chronic postoperative pain relief.…”

Section: Discussionmentioning

confidence: 99%

Preoperative serum circulating microRNAs as potential biomarkers for chronic postoperative pain after total knee replacement

et al. 2020

View full text Add to dashboard Cite

Background Chronic postoperative pain affects approximately 20% of patients with knee osteoarthritis after total knee replacement. Circulating microRNAs can be found in serum and might act as biomarkers in a variety of diseases. The current study aimed to investigate the preoperative expression of circulating microRNAs as potential predictive biomarkers for the development of chronic postoperative pain in the year following total knee replacement. Methods Serum samples, collected preoperatively from 136 knee osteoarthritis patients, were analyzed for 21 circulatory microRNAs. Pain intensity was assessed using a visual analog scale before and one year after total knee replacement. Patients were divided into a low-pain relief group (pain relief percentage <30%) and a high-pain relief group (pain relief percentage >30%) based on their pain relief one year after total knee replacement, and differences in microRNAs expression were analyzed between the two groups. Results We found that three microRNAs were preoperatively dysregulated in serum in the low-pain relief group compared with the high-pain relief group. MicroRNAs hsa-miR-146a-5p, -145-5p, and -130 b-3p exhibited fold changes of 1.50, 1.55, and 1.61, respectively, between the groups (all P values < 0.05). Hsa-miR-146a-5p and preoperative pain intensity correlated positively with postoperative pain relief (respectively, R = 0.300, P = 0.006; R = 0.500, P < 0.001). Discussion This study showed that patients with a low postoperative pain relief present a dysregulation of circulating microRNAs. Altered circulatory microRNAs expression correlated with postoperative pain relief, indicating that microRNAs can serve as predictive biomarkers of pain outcome after surgery and hence may foster new strategies for preventing chronic postoperative pain after total knee replacement (TKR).

show abstract

Section: Discussionmentioning

confidence: 99%

Preoperative serum circulating microRNAs as potential biomarkers for chronic postoperative pain after total knee replacement

et al. 2020

View full text Add to dashboard Cite

show abstract

“…After protein quantification via the BCA method, protein samples were mixed with loading buffer and then denatured at 100°C for 5 min. The protein samples were separated by SDS-PAGE as previously described ( 15 ). In brief, equal amount of protein (30–50 μg) were loaded into 8%, 10% or 12% gel for electrophoresis and then transferred onto PVDF membrane (Millipore).…”

Section: Methodsmentioning

confidence: 99%

Antidiabetic Effect of Artemether in Db/Db Mice Involves Regulation of AMPK and PI3K/Akt Pathways

et al. 2020

View full text Add to dashboard Cite

The traditional Chinese medicine has long been used in the treatment of diabetes, one major disease threatening the public health. It has been reported that artemether exerts antidiabetic effects on type 2 diabetes in db/db mice, however the underlying mechanisms remain unknown. In the present study, we show that artemether regulates expression of related enzymes participating in the glucose and lipid metabolism in the liver of db/db mice, which could at least partly explain the improved glucose and lipid metabolism in artemether-treated mice. Additionally, artemether also regulates expression of glycogen synthesis related enzymes in the skeletal muscle of db/db mice, supporting its promotive role in glycogen synthesis. Mechanistically, artemether activates AMPK pathway as well as PI3K/Akt pathway in the liver and skeletal muscle of db/db mice, suggesting that these two signaling pathways are both involved in the antidiabetic effects of artemether on type 2 diabetes in db/db mice. In conclusion, our study connects the antidiabetic effects of artemether to the regulation of metabolic enzymes and signaling pathways, and also provides molecular basis for the potential application of artemether in treating type 2 diabetes.

show abstract

“…Treatment of a currently clinical-trialed DN drug, Atrasentan (an endothelin-1 receptor antagonist) reduced miR-21 expression, restored the Foxo1 expression, and thus promoted renal autophagy (Wang et al, 2019a). Therefore, following TGFB response, majority of these downstream network of miRNA activates the PI3K/AKT/mTOR pathway, by targeting the PTEN (miR-216a, miR-217, miR-21, miR-22, miR-25, and miR-214) (Kato et al, 2009;Denby et al, 2011;Li et al, 2017a;, collagen type I alpha1 (COLIA1), Collagen IV (COL4), and Fibronectin (FN) (miR-130b) (Ma et al, 2019), PI3K inhibitor (Fog2) (miR-200 family) (Park et al, 2013), Tyrosine 3monooxygenase (Ywhab) (miR-451) (Zhang et al, 2012), DEP domain-containing mTOR-interacting protein (Deptor) (miR-181a) (Maity et al, 2018), Protein canopy homolog-1 (Cnpy1) (miR-370) (Yu et al, 2019), high−mobility group AT−hook-2, Hmga2 (let-7a-5p) (Wang et al, 2019c), Insulin receptor substrate-2, IRS2 (miR-141) (Li et al, 2018b), forkhead box proteins, and Foxo1 (miR-382) (Wang et al, 2018c) genes. Some of these miRNAs are found in circulating bio-fluids and therefore, could potentially be as biomarkers.…”

Section: Micrornas In Renal Hypertrophy and Ecm Accumulationmentioning

confidence: 99%

Interactions Among Non-Coding RNAs in Diabetic Nephropathy

et al. 2020

View full text Add to dashboard Cite

Diabetic Nephropathy (DN) is the most common cause of End-stage renal disease (ESRD). Although various treatments and diagnosis applications are available, DN remains a clinical and economic burden. Recent findings showed that noncoding RNAs (ncRNAs) play an important role in DN progression, potentially can be used as biomarkers and therapeutic targets. NcRNAs refers to the RNA species that do not encode for any protein, and the most known ncRNAs are the microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). Dysregulation of these ncRNAs was reported before in DN patients and animal models of DN. Importantly, there are some interactions between these ncRNAs to regulate the crucial steps in DN progression. Here, we aimed to discuss the reported ncRNAs in DN and their interactions with critical genes in DN progression. Elucidating these ncRNAs regulatory network will allow for a better understanding of the molecular mechanisms in DN and how they can act as new biomarkers for DN and also as the potential targets for treatment.

show abstract

MiR‐130b increases fibrosis of HMC cells by regulating the TGF‐β1 pathway in diabetic nephropathy

Abstract: Basement membrane thickening, glomerular hypertrophy, and deposition of

Cited by 19 publications

References 28 publications

Preoperative serum circulating microRNAs as potential biomarkers for chronic postoperative pain after total knee replacement

Preoperative serum circulating microRNAs as potential biomarkers for chronic postoperative pain after total knee replacement

Antidiabetic Effect of Artemether in Db/Db Mice Involves Regulation of AMPK and PI3K/Akt Pathways

Interactions Among Non-Coding RNAs in Diabetic Nephropathy

Contact Info

Product

Resources

About