miR-133b inhibits glioma cell proliferation and invasion by targeting Sirt1

Li, Chuntao; Zhi-xiong, Liu; Yang, Kui; Chen, Xin; Zeng, Yu; Liu, Jinfang; Li, Zhenyan; Liu, Yunsheng

doi:10.18632/oncotarget.9198

Cited by 51 publications

(45 citation statements)

References 35 publications

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“…In recent years, miRNAs have been reported to be aberrantly expressed in various types of human cancers, such as glioma (32)(33)(34). Dysregulated expression of miRNAs is often correlated with malignant biological behaviours of glioma, such as rapid growth, metastasis, apoptosis inhibition, radiation and chemotherapy resistance (10,35).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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Glioma is the most common and aggressive type of primary malignant brain tumour. Increasing evidence has revealed that microRNAs play important roles in multiple biological processes related to glioma occurrence, development, diagnosis, treatment and prognosis. MicroRNA-202 (miR-202) has been studied in several types of human cancer, whereas the biological roles of miR-202 in glioma remain unknown. The present study, aimed to investigate the expression, clinical significance and biological roles of miR-202 in glioma, as well as its underlying molecular mechanism. We found that miR-202 was significantly downregulated in glioma tissues and cell lines. Low miR-202 expression was associated with Karnofsky performance status (KPS) score and World Health Organization (WHO) grade of glioma patients. Functional assays revealed that ectopic expression of miR-202 inhibited cell proliferation, migration and invasion of glioma. In addition, metadherin (MTDH) was identified as a direct target gene of miR-202 in glioma through bioinformatic analysis, luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. Furthermore, MTDH expression was upregulated and negatively correlated with miR-202 expression in clinical glioma tissues. MTDH knockdown had similar roles to miR-202 overexpression in glioma cells. Rescue experiments revealed that upregulation of MTDH reversed the suppression of glioma cell growth and metastasis by miR-202. Moreover, miR-202 impaired the PI3K/Akt and Wnt/β-catenin pathways. These results highlight the tumour-suppressive effect of miR-202 in glioma, thereby suggesting that miR-202 may be a potential therapeutic target for the treatment of patients with this malignancy.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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“…Since we also demonstrate that CXCL10/IP-10 is a validated miR-503 target, down-regulation of this miRNA may explain, at least in part, the observed CXCL10/IP-10 up-regulation. In the recent few years miR-503 has been found to exert opposite effects in different cancer settings; namely it shows a protective role by inhibiting proliferation and inducing apoptosis in glioma [62] and in prostate cancer [63], while on the contrary its down-regulation has shown a protective role in esophagus carcinoma [64] and osteosarcoma [65]. Additional data indicate contrasting effect of miR-503 in colon carcinoma [66, 67].…”

Section: Discussionmentioning

confidence: 99%

PDGFR-alpha inhibits melanoma growth via CXCL10/IP-10: a multi-omicsapproach

et al. 2016

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Melanoma is the most aggressive skin-cancer, showing high mortality at advanced stages. Platelet Derived Growth Factor Receptor-alpha (PDGFR-alpha) potently inhibits melanoma- and endothelium-proliferation and its expression is significantly reduced in melanoma-biopsies, suggesting that melanoma progression eliminates cells expressing PDGFR-alpha. In the present study transient overexpression of PDGFR-alpha in endothelial (HUVEC) and melanoma (SKMel-28, A375, Preyer) human-cells shows strong anti-proliferative effects, with profound transcriptome and miRNome deregulation. PDGFR-alpha overexpression strongly affects expression of 82 genes in HUVEC (41 up-, 41 down-regulated), and 52 genes in SKMel-28 (43 up-, 9 down-regulated). CXCL10/IP-10 transcript showed up to 20 fold-increase, with similar changes detectable at the protein level. miRNA expression profiling in cells overexpressing PDGFR-alpha identified 14 miRNAs up- and 40 down-regulated, with miR-503 being the most down-regulated (6.4 fold-reduction). miR-503, miR-630 and miR-424 deregulation was confirmed by qRT-PCR. Interestingly, the most upregulated transcript (i.e., CXCL10/IP-10) was a validated miR-503 target and CXCL10/IP-10 neutralization significantly reverted the anti-proliferative action of PDGFR-alpha, and PDGFR-alpha inhibition by Dasatinb totally reverted the CXCL10/IP10 induction, further supporting a functional interplay of these factors. Finally, integration of transcriptomics and miRNomics data highlighted several pathways affected by PDGFR-alpha.This study demonstrates for the first time that PDGFR-alpha strongly inhibits endothelial and melanoma cells proliferation in a CXCL10/IP-10 dependent way, via miR-503 down-regulation.

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“…Through recent years, various genes and miRNAs are introduced to be participated in glioma tumorigenesis. From decades up to know, miRNAs are considered as an important biomarker, which are dysregulated in many types of disorders including glioma [24][25][26]. Change in expression of miRNAs is associated with various malignant biological behaviors in glioma such as cell survival, metastasis, proliferation, resistance to chemotherapy and radiation [11,27].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miRNA-202 promotes apoptosis and inhibits migration of glioma cell line via downregulation of ROCK1 gene

Behzadi

Hatami

Alian

et al. 2020

Preprint

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Background: We evaluated role(s) of miR-202 in glioma cell lines, its effect on ROCK1 expression, and also evaluation of apoptosis and migration of human glioma cell line after transfection with miR-202 mimics and inhibitors. Material and methods: The cell lines were transfected with mimic, inhibitor and NC of miR-202. Reverse transcription polymerase chain reaction (RT-PCR) was conducted to evaluate the expression of miR‐202 and ROCK1 . Western blot was performed to detect the protein level of ROCK1. Furthermore, MTT and wound healing assay were performed to evaluate the effects of miR-202 on apoptosis and migration of human glioma cell line, respectively. Results: miR-202 showed a significantly decrease in human glioma cell lines, compared with the NHA cell line (P<0.05). The ROCK1 expression was significantly upregulated in glioma cell lines, compared with the NHA cell line ( P <0.05). Furthermore, a negative correlation was observed between expression of ROCK1 and miR-202 ( P =0.01, r=-0.426). The mRNA and protein levels of ROCK1 were decreased in U87 cell line in miR-202 mimics group, compared with mimic NC group (P<0.05). In addition, apoptosis was significantly increased in miR-202 mimics, compared with the NC group in U87 cell line at 72 and 96 h (P<0.05). Furthermore, invasion showed a significant decrease in miR-202 mimic group, compared with U87 cell line at 24 and 48 h (P<0.05). Conclusions: The miR-202 could serve as a tumour-suppressor miRNA in glioma. Therefore, targeting ROCK1 by miR-202 may increase improve disease outcome and could be considered as a potential therapeutic target for glioma patients.

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miR-133b inhibits glioma cell proliferation and invasion by targeting Sirt1

Cited by 51 publications

References 35 publications

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

PDGFR-alpha inhibits melanoma growth via CXCL10/IP-10: a multi-omicsapproach

Upregulation of miRNA-202 promotes apoptosis and inhibits migration of glioma cell line via downregulation of ROCK1 gene

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